Biología (trabajo de grado)
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Ítem Herramienta de evaluación del bienestar animal en Cebus albifrons bajo el cuidado humano(Universidad EAFIT, 2024) Saldarriaga Claros, Paula Alexandra; Madrigal Rodríguez, ElisaÍtem Aproximación electroquímica y espectrofotométrica de la capacidad antioxidante de Synsepalum dulcificum : un análisis comparativo(Universidad EAFIT, 2024) Patiño Gallego, José Alejandro; Blandón Naranjo, Lucas Hernán; Quintero Quiroz, JuliánÍtem Evaluación del medio de cultivo sobre la producción de macromoléculas funcionales en la cepa Chlorella sorokiniana UTEX 1230 como posible suplemento alimenticio para gallinas ponedoras(Universidad EAFIT, 2024) Múnera Betancourt, Amalia; Miranda Parra, Alejandra MaríaAlgae and microalgae are considered viable sources of protein for the poultry industry, used in animal nutrition to replace soybean meal (SBM) due to their high content of protein, fatty acids, vitamins, minerals and pigments. This study evaluates the production of functional macromolecules in the microalgae strain Chlorella sorokiniana UTEX 1230 as a supplement for laying hens. Cell growth was examined in different culture media with stimulant clinker powder, evaluating the bromatological composition, exopolysaccharides, fatty acid profile and in vitro digestibility, as well as the cost of production. These preliminary analyses are key to the implementation of microalgae as a protein source in poultry feed and warrant further in vivo testing.Ítem Evaluación del medio de cultivo sobre la producción de macromoléculas funcionales en la cepa Chlorella sorokiniana UTEX 1230 como posible suplemento alimenticio para gallinas ponedoras(Universidad EAFIT, 2024-05-23) Múnera Betancourt, Amalia; Miranda Parra, Alejandra MaríaAlternative protein sources, specifically algae and microalgae, are considered as viable protein sources for the poultry industry. Currently, different species of algae are used in animal nutrition to replace soybean meal (SBM) due to their high content of protein, fatty acids, and the availability of vitamins, minerals and pigments. Determining the chemical and nutritional composition of a biological formulation, in addition to its digestibility, is the first step in evaluating its suitability for use in poultry feeds. The aim of this work is to evaluate different culture media in the production of functional macromolecules in the microalgae strain Chlorella sorokiniana UTEX 1230 as a possible feed supplement for laying hens. The cell growth of the microalgae was evaluated over time under different culture media and different levels of addition of the stimulant clinker powder, a product of the cement industry. The biomass obtained from this process was evaluated for its bromatological composition, exopolysaccharide content, fatty acid profile, and in vitro digestion using digestive enzymes from monogastric animals, in addition to an evaluation of its production cost. This evaluation represents a preliminary analysis to initially select the mode of production to implement these products as a protein source in poultry feed, and further in vivo testing is warranted.Ítem Formulación de una estrategia de conservación de orquídeas en una reserva natural de bosque alto andino(Universidad EAFIT, 2024) Patiño Baena, Sebastián; Martínez Aguillón, CamilaÍtem Evaluación del potencial del magnetismo ambiental como herramienta para el biomonitoreo de la calidad del aire en Medellín, usando las dos especies de briófitos más abundantes en la ciudad(Universidad EAFIT, 2024) García Arias, Mariana; Benavides Duque, Ana María; Duque Trujillo, José FernandoÍtem Cranial Morphology of the Andean Lizard genus Pholidobolus (Gymnophthalmidae : Cercosaurinae) in Northern Colombia(Universidad EAFIT, 2024) Lozano Arias, Juan Manuel; Garzón Franco, Esteban; Arredondo, Juan Camilo; Arredondo, Juan Camilo; Garzón Franco, EstebanÍtem Caracterización de criterios de muestreo de Megaptera novaeangliae en el hemisferio sur americano para la elección del modelado poblacional(Universidad EAFIT, 2024) Londoño Gallego, Juan Andrés; Catatño López, AlexandraThis paper focuses on characterizing the sampling criteria in population studies of humpback whales (Megaptera novaeangliae) in the American Southern Hemisphere and Southern Ocean, examining key aspects such as sampling periods, type of modeling, sampling employed, geographic region, and potential impacts on population study areas such as migration, abundance, and population estimation; with the objective of understanding the current status of the study of the species and identifying factors that influence population modeling and the establishment of appropriate management and conservation measures. The methodology included a systematic literature review following the PRISMA 2020 protocol, searching Scopus and PubMed and applying inclusion and exclusion criteria to select 67 articles published between 2010 and 2023. A matrix was constructed with bibliographic information characterizing each study according to type of sampling, temporal factor, modeling, geography and impact, identifying the most frequent data in each section. Finally, a decision tree was constructed based on the information in the matrix, suggesting the most appropriate type of model according to the data and methodology used in the population study, acting as a practical guide to facilitate the choice of the appropriate population modeling, considering sampling criteria and other relevant factors identified in the systematic review.Ítem Efectos del déficit de fósforo en plantas de maíz(Universidad EAFIT, 2024) Riascos Rubiano, Catalina; Gilchrist Ramelli, ElizabethÍtem Genomic insights into Pannus brasiliensis CCIBt3594 a freshwater Cyanobacterium from Pantanal, Brazil(Universidad EAFIT, 2023) Betancurt Botero, Natalia; de Fátima Fiore, Marli; Junior Machado, MauricioÍtem Caracterización de la respuesta de Moniliophthora roreri a fungicidas químicos : eficacia y potencial de generar resistencia(Universidad EAFIT, 2023) Serna Muriel, Daniela; Mosquera López, Sandra; Correa Álvarez, JavierÍtem Type locality clarification and redescription of Andinobates opisthomelas (Boulenger, 1899)(Universidad EAFIT, 2023) Durango Cardona, Juan Pablo; Arredondo Salgar, Juan CamiloÍtem Diskriminierung von infektiösen und nicht-infektiösen SARS-CoV-2 Viren mit Hilfe einer zu optimierenden Extraktionsmethode und unter qRT-PCR Anwendung und Verwendung von EMA und PMAxx(Universidad EAFIT, 2023) Sierra Peláez, Susana; Harms, CarstenÍtem Mitigation of Bovine Oocyte Aging Through NMN Supplementation During in vitro Maturation(Universidad EAFIT, 2023) Carrillo Gómez, Andrea Carolina; Torres Osorio, Viviana; Duque Rodríguez, Matteo; Urrego Álvarez, Rodrigo Antonio; Urrego Álvarez, Rodrigo Antonio; Torres Osorio, VivianaÍtem Clonación del gen crtI, procedente de Rhodotorula mucilaginosa EA-BIO010 en cepas de Escherichia coli TOP10, mediante técnicas de ingeniería genética(Universidad EAFIT, 2023) Ospina Mejía, Manuel; Sierra Zapata, Laura; Villanueva Mejía, Diego FernandoReactive oxygen species are unstable molecules that contain oxygen and react easily with other molecules in the cell where they cause damage to lipid molecules, proteins and DNA. To combat them, antioxidants such as beta-carotene have been identified, produced by organisms such as Blakeslea trispora, Dunalliela salina, Rhodotorula glutinis, Rhodotorula mucilaginosa, among others. However, beta-carotene is produced in low amounts compared to other antioxidants by yeast strains such as R. mucilaginosa. It should be noted that many of the methodologies used today for the extraction of antioxidants from plants or microorganisms are inefficient, expensive and environmentally unsustainable. Yeasts of the genus Rhodotorula then become promising candidates for beta-carotene production, since it has been shown that their beta-carotene producing cluster can be expressed heterologously in other organisms to optimize its production. Therefore, this project aimed to establish the first stage of the methodology for the heterologous production of the biosynthetic cluster of Rhodotorula mucilaginosa EA-BIO010 in E. coli TOP10 strains, starting with the cloning of the crtl gene. This new recombinant micro-organism could be used in the future for the sustainable and efficient production of beta-carotenes and contribute to the creation of biological processes for the production of antioxidants for industrial use.Ítem Evaluación de la riqueza taxonómica, riqueza funcional y variabilidad de gremios alimenticios de la avifauna presente en el Refugio de Vida Silvestre Aguas Frías, Medellín(Universidad EAFIT, 2023) Ramírez Valencia, Camila; Pinel Peláez, Nicolás; Cataño López, AlexandraÍtem Desarrollo de ruta de producción de prototipo de probiótico de Bacillus amyloliquefaciens(Universidad EAFIT, 2023) Pino Herrera, María José; Bardales Mendieta, JorgeÍtem Rastreando la Evolución de los Factores de Transcripción TALE : Implicaciones para la diferenciación celular y desarrollo(Universidad EAFIT, 2023) Barbosa Pérez, Andrés; Sánchez Rodríguez, AminaelÍtem Ensayos de germinación y modelo de distribución para Godoya Antioquiensis en Antioquia y regiones aledañas(Universidad EAFIT, 2023) Botero López, Juan José; Martínez Aguillón, Camila; Restrepo Cossío, Leydi ValentinaGodoya antioquiensis is a tree species native to Colombia, distributed throughout the central and western mountain range, between 1600 m above sea level (masl) and 2600 masl. Currently, due to anthropogenic factors and overpopulation that have altered environmental conditions, its populations are under threat. The objective of the present study is to generate data and tools regarding its propagation and potential distribution to promote the conservation of the species. To determine seed viability, a tetrazolium staining test was conducted, revealing a 10% variation in viability between stored and fresh seeds, as well as greater imbibition for the latter. According to the germination results, the best substrate in terms of the quantity of seedlings obtained in the initial phase (30 days) is sand. However, despite Sphagnum presenting a lower result, its seedlings showed better development. Additionally, it is expected that the distribution model obtained will identify potential occurrence zones where the species has not been previously recorded. Moreover, based on the results of the imbibition test, Godoya antioquiensis seeds can withstand prolonged periods of desiccation. However, storing them is not advisable as they may lose germinative vigor. Due to the wide distribution of the species, in the Antioquia department, there is good availability of optimal Andean ecosystems for its reintroduction.Ítem Evaluación del proceso de reintroducción de tres manatíes antillanos (Trichechus manatus manatus) liberados en República Dominicana mediante la caracterización de su dieta(Universidad Eafit, 2023) Villegas Molina, Vanessa; Álvarez Alemán, Anmari; Martínez Aguillón, Camila