Biología (trabajo de grado)
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Publicación Proceso de validación de un método de cuantificación de células vegetativas y esporas de Bacillus spp. en productos probióticos(Universidad EAFIT, 2025) Franco Correa, Juliana; Bardales Mendieta, Jorge AdalbertoÍtem Optimización del cultivo in vitro de Glycine max L. y aislamiento enzimático de sus protoplastos con fines biotecnológicos(Universidad EAFIT, 2024) Pavón Restrepo, Jessica; Villanueva Mejía, Diego FernandoÍtem Producción de Factor de Crecimiento Epidermal Humano (hEGF) por métodos recombinantes(Universidad EAFIT, 2024) González Santos, Andrés Felipe; Corrales García, Ligia LuzHuman Epidermal Growth Factor (hEGF) is a protein naturally produced by skin cells and essential in wound healing and tissue regeneration. In this study, the production of recombinant hEGF was carried out using Escherichia coli as the expression model. The hEGF gene was cloned into the pET32a vector and initially transformed into E. coli XL1-Blue for its amplification. Subsequently, the purified plasmid was used to transform E. coli BL21, a strain optimized for recombinant protein expression. hEGF expression was induced with 1 mM IPTG (Isopropyl ß-D-1-thiogalactopyranoside) at 16 °C for 16 hours, conditions that favored obtaining the protein in soluble form. Purification was performed through a two-stage process: nickel a inity chromatography (Ni-NTA) followed by anion exchange chromatography. The identity and purity of the protein were confirmed by performing acrylamide gel electrophoresis (SDS-PAGE) and Western blot based on the use of anti-His antibodies. This protocol allowed obtaining recombinant hEGF in its soluble and active form, establishing an e icient methodology for its production at a laboratory scale.Ítem Inducción de callogénesis y establecimiento del cultivo de células en suspensión de Bixa orellana L. (achiote)(Universidad EAFIT, 2024) Gómez Sánchez, David Alejandro; Villanueva Mejía, Diego Fernando; Restrepo Osorio, Martha CatalinaÍtem Identification of efficient 1,4-beta-glucanases and development of an enzymatic screening assay(Hochschule Bremerhaven, 2024) Avendaño Moreno, David Alexander; Schmidt, MarioÍtem Dinámica de intoxicaciones por sustancias psicoactivas en Antioquia antes y después de la pandemia (2015-2023)(Universidad EAFIT, 2024) Flórez López, Leidy Juliana; Cataño López, AlexandraÍtem Evaluación del potencial bioestimulante del crecimiento de plantas de tres especies de microalgas(Universidad EAFIT, 2024) Zapata Giraldo, María Camila; Miranda Parra, Alejandra María; Sáez Vega, Alex ArmandoÍtem Identificación de mutaciones en regiones promotoras en un espécimen de la raza Gyr (Bos indicus) con desorden del desarrollo sexual(Universidad EAFIT, 2024) Cadavid Morales, Simón; Guzmán Zapata, Daniel JaimeÍtem Evolución y función putativa de la heterogeneidad fenotípica en Bacillus tequilensis EA-CB0015(Universidad EAFIT, 2024) Ramírez Molina, Federico Andrés; Villegas Escobar, Valeska; Aguilera Gálvez, CarolinaÍtem Description of five new species of the Bacillaceae and Paenibacillaceae family isolated from Musa sp. in Colombia(Universidad EAFIT, 2024) Vidal Cataño, Ana María; Martínez Cerón, Juan Manuel; Díaz Nieto, Juan Fernando; Villegas Escobar, Valeska; Díaz Nieto, Juan Fernando; Villegas Escobar, ValeskaÍtem Heterobranquios marinos (Gastropoda: Heterobranchia) de Colombia : lista actualizada basada en una revisión sistemática y de registros biológicos(Universidad EAFIT, 2024) Tamayo Gaviria, Evelyn; Londoño Mesa, Mario HernánMarine heterobranchs, commonly called sea slugs, are gastropod molluscs with a great diversity of morphologies, which include shelled and non-shelled organisms. They play an active role in the food webs of the ecosystems they inhabit, due to their varied diet and symbiotic relationship with other species, which allows them to modify their distribution. Colombia has coasts on two oceans (Atlantic and Pacific with different geological and biological characteristics, which allows it to be divided into 18 marine Ecoregions. Although research on heterobranchs in the country has increased recently, the 3 species list for both coasts has not been updated since 2007; therefore, the current diversity of marine slugs is unknown. This research aims to update the list of marine heterobranchs (Gastropoda: Heterobranchia) of Colombia. The species search was done systematically, following PRISMA recommendations, and with a search of biological records in SiB Colombia, which were compared to obtain the most complete possible list of marine heterobranch species of Colombia. A total of 250 species, 125 genus and 60 families were found; of these species, 147 have been recorded for the Colombian Caribbean and 110 for the Colombian Pacific. In addition, 6 species were found to be shared by both coasts, with an Amphi-American distribution. The Sanquianga, Naya and Tumaco ecoregions should be prioritized in future research because of the few records known.Ítem Evolución y función putativa de la heterogeneidad fenotípica en Bacillus tequilensis EA-CB0015(Universidad EAFIT, 2024) Ramírez Molina, Federico Andrés; Villegas Escobar, Valeska; Aguilera Galvez, CarolinaÍtem Aislamiento de bacterias formadoras de endosporas similares a Bacillus de diferentes suelos de Colombia : desarrollo de banco de cepas(Universidad EAFIT, 2024) Pérez Muñoz, Alejandro; Bardales Mendieta, Jorge AdalbertoÍtem Existen huellas microbianas características de la microbiota facial, en una comunidad de adultos con acné vulgar de Antioquia, Colombia(Universidad EAFIT, 2024) Pérez Arboleda, Verónica; Sierra Zapata, Laura; Gómez Mesa, LauraÍtem Identificación de patógenos asociados al síndrome febril agudo indiferenciado en pacientes de la ciudad de Cúcuta, Norte de Santander(Universidad EAFIT, 2024) González Ramírez, Manuel; Pérez Restrepo, Laura SilvanaÍtem Unveiling Genetic Diversity : Construction and Analysis of the Moniliophthora roreri Pangenome”(Universidad EAFIT, 2023) Gallego-Rendón, Isabella; Riaño-Pachón, Diego MauricioThe cacao pathogen Moniliophthora roreri poses a significant threat to global cacao production. This is due to its distinct dispersal ability, its propensity to infect a wide range of cacao cultivars, its adaptability to different ecological niches, and its high pathogenicity. To develop effective control strategies, a deeper understanding of its genetic diversity and functional capabilities is critical. The objective of this study was to perform a pangenomic analysis of 22 M. roreri genomes to gain insights into its genetic composition and potential functional properties.Ítem Identificación de los valores objeto de conservación de Reserva la Rivera, Filandia, Quindío(Universidad EAFIT, 2024) Gómez Tirado, Esteban; Giraldo Salazar , Santiago; Díaz Pulido, AngélicaConservation values (CVs) are a limited number of species, communities or ecosystems that are chosen to represent the biodiversity of a landscape or protected area. They are objectives because they can be pillars in the conservation of ecosystems and their biodiversity, can be monitored over time, and account for the fulfillment of the conservation objectives of a protected area. The new conservation figures based on areas and that are constituted from civil society also require the implementation of strategies and conservation plans focused on CV. Our attention is focused on Reserva La Rivera, located in the north of the department of Quindío and directly bordering the Otún Quimbaya Fauna and Flora Sanctuary, which is part of the buffer zone of Los Nevados National Natural Park. It is a private conservation project with 345 hectares distributed between forests in different stages of regeneration and grasslands, and is located in one of the most important biodiversity hotspots in the world, the tropical Andes. For this study, biotic inventories of birds, mammals, reptiles and amphibians were carried out, taking into account historical records, collected specimens and field sampling in Reserva La Rivera; 308 species of birds, 30 of mammals, 13 of reptiles and 9 of amphibians were recorded and the conservation values were prioritized according to five criteria: heterogeneity, area of distribution, vulnerability, function and socioeconomic value. Five species were selected as VOC, represented by three birds, one mammal and one reptile. This diversity reflects the richness of the study area, its importance in the objectives of other protected areas and the connectivity in its buffer zones.Ítem First Draft Assembly and Annotation of the Genome of the Cadmium-Resistant Fungus Talaromyces santanderensis HF05 using Oxford Nanopore Sequencing : First Steps Towards Understanding its Cadmium Resistance(Universidad EAFIT, 2024) Picon Cossio, Juan José; Correa Álvarez, JavierÍtem Detección molecular de endosimbiontes Cardinium y Microsporidia en insectos vectores de la subfamilia Phlobotominae provenientes de áreas de alta transmisión de leishmaniasis(Universidad EAFIT, 2024) Tobón Vélez , Juan Pablo; Moreno Herrera, Claudia XimenaÍtem Compared anatomy of the tadpoles of Hyalinobatrachium tatayoi Castroviejo-Fischer et al., 2007 and Teratohyla pulverata (Peters, 1873) (Anura: Centrolenidae)(Universidad EAFIT, 2024) Abreu-Acosta, Daniel; Arredondo Salgar, Juan Camilo