Biología (trabajo de grado)
URI permanente para esta colección
Examinar
Envíos recientes
Publicación Cultivable Microbial Community Structure Along a Light Gradient in a Tropical Volcaniclastic Cave(Universidad EAFIT, 2026-05-26) Correa-Gallego, Sebastián; Pinel Peláez, NicolásCave environments compress sharp changes in light, energy input, surface connectivity, and substrate heterogeneity into short spatial gradients. This makes them powerful natural systems for examining microbial responses to environmental heterogeneity. Yet the cultivable microbial fraction of tropical volcaniclastic caves remains poorly resolved. Organal-type pseudokarstic caves of the Colombian Andes are especially underrepresented in cave microbiology, despite their distinctive geomorphology and hydrological connection to montane landscapes. Here, I tested whether sector identity along the light-defined zonation of the Organal San Antonio, a tropical volcaniclastic cave at ~2350 m a.s.l. in Támesis, Antioquia, is associated with differences in the R2A-recoverable microbial fraction of cave sediments. Two cultivation experiments were conducted: an initial binary Light–Dark comparison and a primary three-sector experiment resolving Entrance, Transition, and Dark zones with aliquot-level gravimetric normalization, 15 °C incubation, and a twenty-three-cluster operational phenotypic crosswalk. In the primary experiment, cultivable density followed a step-like pattern across the gradient: Entrance and Transition sediments supported approximately 5.1–5.6 × 10⁵ CFU g⁻¹ field-moist sediment, whereas the fully aphotic Dark sector supported approximately 8.9 × 10⁴ CFU g⁻¹, a roughly sixfold reduction (η² = 0.652). Local morphotype-cluster richness and evenness showed no detectable sector-level differences, but community composition partitioned strongly with zonation (PERMANOVA R² = 0.573, p = 0.001) under balanced multivariate dispersion. The Transition sector supported a distinctive set of zone-restricted phenotypic clusters, contributing approximately 27% of its cultivable community, while the Dark sector combined reduced density with moderate abundance of aphotic-restricted clusters. Preliminary Gram, KOH, catalase, and microscopic characterization of representative isolates revealed Gram-level uncertainty and cellular heterogeneity within several cross-sector colony phenotypes, confirming that the morphotype crosswalk is useful for community-level comparison but not equivalent to taxonomic resolution. These results establish a first spatially resolved cultivable baseline for an organal-type tropical volcaniclastic cave and indicate that sector identity along a light-defined cave gradient is associated with lower cultivable density in the aphotic sector and strong morphotype-level compositional turnover, without a parallel decline in local morphotype-cluster diversity. The study defines the scale at which these patterns are valid: the aerobic, low-temperature, dilute-R2A cultivable fraction of sediment-associated microorganisms.Publicación Comparative analysis of cultivable bacterial communities in raw milk and Antioquian soft cheese(Universidad EAFIT) Gómez García, Valeria; Villegas Escobar, Valeska; Yepes Medina, Verónica; Pinel Peláez, Nicolás; NovonesisPublicación Evaluación de un método para el análisis del metaboloma intestinal con fines de nutrición de precisión : estudio piloto Biomatest Plus(Universidad EAFIT, 2025-11-13) López-González, Mariana; Sierra Zapata, Laura; Arrieta Echeverri, María Clara; Astrolab Bio.Publicación Dinámicas de competencia en poblaciones con heterogeneidad fenotípica de Bacillus tequilensis EA-CB0015(Universidad EAFIT, 2025-11-10) Acevedo Quintero, Silvana; Villegas Escobar, Valeska; Aguilera Gálvez, Carolina; Universidad EAFITPublicación Odonatos de la Reserva Aguas Frías, Medellín, Antioquia(Universidad EAFIT, 2025) Merchán Correa, Juan Pablo; Bota Sierra, Cornelio Andrés; Alcaldía de Medellín, Secretaría del Medio AmbientePublicación Vía de señalización NOTCH a nivel vascular en CADASIL y Alzheimer Familiar(Universidad EAFIT, 2025) Rivera Córdoba, Samuel David; Posada Duque, Rafael Andrés; Pineda López, Lina GiselaPublicación Especies arbóreas y arbustivas con potencial restaurador en el bosque premontano de Antioquia(Universidad EAFIT, 2025) Tenelanda Osorio, Mariana; Carmona Duque, Deysi TatianaPublicación Proceso de validación de un método de cuantificación de células vegetativas y esporas de Bacillus spp. en productos probióticos(Universidad EAFIT, 2025) Franco Correa, Juliana; Bardales Mendieta, Jorge Adalberto; Iluma AlliancePublicación Optimización del cultivo in vitro de Glycine max L. y aislamiento enzimático de sus protoplastos con fines biotecnológicos(Universidad EAFIT, 2024) Pavón Restrepo, Jessica; Villanueva Mejía, Diego FernandoPublicación Producción de Factor de Crecimiento Epidermal Humano (hEGF) por métodos recombinantes(Universidad EAFIT, 2024) González Santos, Andrés Felipe; Corrales García, Ligia LuzHuman Epidermal Growth Factor (hEGF) is a protein naturally produced by skin cells and essential in wound healing and tissue regeneration. In this study, the production of recombinant hEGF was carried out using Escherichia coli as the expression model. The hEGF gene was cloned into the pET32a vector and initially transformed into E. coli XL1-Blue for its amplification. Subsequently, the purified plasmid was used to transform E. coli BL21, a strain optimized for recombinant protein expression. hEGF expression was induced with 1 mM IPTG (Isopropyl ß-D-1-thiogalactopyranoside) at 16 °C for 16 hours, conditions that favored obtaining the protein in soluble form. Purification was performed through a two-stage process: nickel a inity chromatography (Ni-NTA) followed by anion exchange chromatography. The identity and purity of the protein were confirmed by performing acrylamide gel electrophoresis (SDS-PAGE) and Western blot based on the use of anti-His antibodies. This protocol allowed obtaining recombinant hEGF in its soluble and active form, establishing an e icient methodology for its production at a laboratory scale.Publicación Inducción de callogénesis y establecimiento del cultivo de células en suspensión de Bixa orellana L. (achiote)(Universidad EAFIT, 2024) Gómez Sánchez, David Alejandro; Villanueva Mejía, Diego Fernando; Restrepo Osorio, Martha CatalinaPublicación Identification of efficient 1,4-beta-glucanases and development of an enzymatic screening assay(Hochschule Bremerhaven, 2024) Avendaño Moreno, David Alexander; Schmidt, Mario; Kaesler GmbHPublicación Dinámica de intoxicaciones por sustancias psicoactivas en Antioquia antes y después de la pandemia (2015-2023)(Universidad EAFIT, 2024) Flórez López, Leidy Juliana; Cataño López, AlexandraPublicación Evaluación del potencial bioestimulante del crecimiento de plantas de tres especies de microalgas(Universidad EAFIT, 2024) Zapata Giraldo, María Camila; Miranda Parra, Alejandra María; Sáez Vega, Alex ArmandoPublicación Identificación de mutaciones en regiones promotoras en un espécimen de la raza Gyr (Bos indicus) con desorden del desarrollo sexual(Universidad EAFIT, 2024) Cadavid Morales, Simón; Guzmán Zapata, Daniel JaimePublicación Evolución y función putativa de la heterogeneidad fenotípica en Bacillus tequilensis EA-CB0015(Universidad EAFIT, 2024) Ramírez Molina, Federico Andrés; Villegas Escobar, Valeska; Aguilera Gálvez, CarolinaPublicación Description of five new species of the Bacillaceae and Paenibacillaceae family isolated from Musa sp. in Colombia(Universidad EAFIT, 2024) Vidal Cataño, Ana María; Martínez Cerón, Juan Manuel; Díaz Nieto, Juan Fernando; Villegas Escobar, Valeska; Díaz Nieto, Juan Fernando; Villegas Escobar, ValeskaPublicación Heterobranquios marinos (Gastropoda: Heterobranchia) de Colombia : lista actualizada basada en una revisión sistemática y de registros biológicos(Universidad EAFIT, 2024) Tamayo Gaviria, Evelyn; Londoño Mesa, Mario HernánMarine heterobranchs, commonly called sea slugs, are gastropod molluscs with a great diversity of morphologies, which include shelled and non-shelled organisms. They play an active role in the food webs of the ecosystems they inhabit, due to their varied diet and symbiotic relationship with other species, which allows them to modify their distribution. Colombia has coasts on two oceans (Atlantic and Pacific with different geological and biological characteristics, which allows it to be divided into 18 marine Ecoregions. Although research on heterobranchs in the country has increased recently, the 3 species list for both coasts has not been updated since 2007; therefore, the current diversity of marine slugs is unknown. This research aims to update the list of marine heterobranchs (Gastropoda: Heterobranchia) of Colombia. The species search was done systematically, following PRISMA recommendations, and with a search of biological records in SiB Colombia, which were compared to obtain the most complete possible list of marine heterobranch species of Colombia. A total of 250 species, 125 genus and 60 families were found; of these species, 147 have been recorded for the Colombian Caribbean and 110 for the Colombian Pacific. In addition, 6 species were found to be shared by both coasts, with an Amphi-American distribution. The Sanquianga, Naya and Tumaco ecoregions should be prioritized in future research because of the few records known.Publicación Evolución y función putativa de la heterogeneidad fenotípica en Bacillus tequilensis EA-CB0015(Universidad EAFIT, 2024) Ramírez Molina, Federico Andrés; Villegas Escobar, Valeska; Aguilera Galvez, CarolinaPublicación Aislamiento de bacterias formadoras de endosporas similares a Bacillus de diferentes suelos de Colombia : desarrollo de banco de cepas(Universidad EAFIT, 2024) Pérez Muñoz, Alejandro; Bardales Mendieta, Jorge Adalberto; Alura S.A.S.