Biología (trabajo de grado)
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Ítem Actividad anti-leishmania in vitro de los extractos naturales obtenidos de Crescentia Cujete y Piper Aduncum(Universidad EAFIT, 2019) Posada Bermúdez, Angélica; Robledo Restrepo, Sara MaríaThe present study aimed to measure in vitro anti-Leishmania activity of leaf extract in water, leaves plus stems in water and leaves in ethanol obtained from Crescentia cujete and Piper aduncum, to achieve this, cytotoxicity was calculated which for all extracts it was high (LC50: <100 μg/mL). The anti-Leishmania activity was also determined and even though values are in the high activity range (EC50: <25 μg/mL), by taking into account low results of selectivity index (SI), it is possible to affirm that it is due to cell death, hence of parasites, although their death is not because of extracts. In conclusion, extracts present more cytotoxicity than activityÍtem Actividad antioxidante de un consorcio microbiano derivado de kéfir de agua : validación de su funcionalidad postbiótica(Universidad EAFIT, 2020) Fernández Trujillo, David; Sierra Zapata, Laura; Arrieta Echeverri, María ClaraÍtem Actividad antiparasitaria de lipopéptidos producidos por Bacillus tequilensis EA-CB0015 sobre Leishmania (Viannia) braziliensis, Trypanozoma cruzi y Plasmodium falciparum(Universidad Eafit, 2020) Castillo Cardona, Kelly Johana; Londoño Barbarán, Camila; Robledo Restrepo, Sara María; Villegas Escobar, ValeskaÍtem Actividad biológica y química en componentes de Gymnopilus subpurpuratus(Universidad EAFIT, 2019) Góez Estupiñán, Sara; Saez Vega, Alex ArmandoÍtem Análisis del genoma de influenza aviar H7N3 de la Epizootia 2012-2015 en México(Universidad EAFIT, 2017) Figueroa Varela, Paula Andrea; Martínez Barnetche, JesúsÍtem Análisis metagenómico y perfil metabólico de la comunidad bacteriana del proceso fermentativo artesanal de cacao de una finca cacaocultora de Santander, Colombia(Universidad EAFIT, 2020) Angulo Patrón, Giany Lucia; Correa Álvarez, JavierÍtem Analysis of TYK2 P1104A variant in a cohort of colombian patients with pulmonary tuberculosis(Universidad EAFIT, 2020) Guzmán Zuluaga, Laura Cristina; Arias Sierra, Andrés Augusto; Barrera Robledo, Luis FernandoTuberculosis continues to be one of the main causes of death worldwide with around 10 million people infected annually, which is a direct problem for public health. Data that has accumulated over decades suggests that variability in human susceptibility to tuberculosis disease has a strong genetic component. Both primary and acquired immunodeficiencies (PID) are strongly related to broad infectious phenotypes that include the full range of diseases caused by the infectious agent Mycobacterium tuberculosis. Recently it was found that a PID found in up to 1/600 individuals of European descent, in fact a variant was found that affects the catalytic activity of TYK2 therefore selectively alters the immunity to IFN-g mediated by IL-23 while the responses to IL-10, IL-12, and IFN-a / b are intact. These PIDs are only expressed symptomatically after exposure to Mycobacterium tuberculosis. However, we hypothesize that homozygous for the P1104A variant or compound heterozygosity in this same TYK2 gene can confer a selective response, thus predisposing to TB. This study intends to 1. Identify the variant P1104A of the TYK2 gene in Colombian patients with pulmonary tuberculosis, 2. Identify other rare or common variants in the TYK2 gene in patients with pulmonary tuberculosis, 3. Correlating data from the Colombian population with what has been found in other populations.Ítem Los Andes actuó como "elevador pasivo" en la diversificación de las ranas de cristal (Anura: Centrolenidae)(Universidad EAFIT, 2020) Rueda Isaza, Yehimy Xilena; Díaz Nieto, Juan Fernando; Barrientos Correa, Lucas SantiagoÍtem Antagonismo de microorganismos aislados a partir de la borra de café, generada en la Universidad EAFIT, sobre el crecimiento del hongo Pleurotus sp.(Universidad EAFIT, 2021) Caro Miranda, Vanesa; Jaramillo Mazo, Claudia YulietÍtem Aporte sobre la innovación en la industria láctea del oriente antioqueño(Universidad EAFIT, 2020) Jaramillo Muñoz, Daniela; Mesa Cano, Jorge HernánÍtem Application of sensitivity- and uncertainty-based techniques for the assessment of epidemiological models in real-life study cases(Universidad EAFIT, 2019) Rojas Díaz, Daniel; Vélez Sánchez, Carlos Mario; Puerta Yepes, María EugeniaUncertainty analysis (UA) and sensitivity analysis (SA) are tools to assess and to quantify the uncertainty spread from the input factors (parameters and initial states) to the model output, taking into account the effect of the interactions among those factors. Throughout the following works, I treat UA as a graphical assessment of uncertainty propagation based on Monte Carlo simulation, which makes it possible to state a range for the model output in cases where it is considered relevant. On the other hand, I privilege the global approach for SA instead of the local one, since the first attempts to quantify the uncertainty contribution of the model factors in their entire distribution range while the second one is only informative for a single locus in the distribution. In this way, when applying global UA/SA on a model, it is possible to identify those factors that mostly determine the model behavior. Furthermore, I have noticed that the concepts and principles of UA/SA are associated with other main tasks in modeling, as factors estimation and confidence intervals achievement: Briefly, those non-identifiable factors in a model (factors whose value can not be estimated uniquely from some information about output data) should belong to the categories of non-sensible or sensitive but correlated from SA; and, the sub-space of the space of factors where the factors may jointly exist producing a model output that fits, in some extent, to a given output data, could be approximately estimated with UA-based approaches, constituting a new kind of confidence interval. Thus, in this compendium, I present five works related to the applications of UA/SA techniques as well as its relevance. The objective of those applications evolves from the most logically immediate to some derived and more complex ones, though still preserving the model pertinence as a central topic.Ítem Aproximación electroquímica y espectrofotométrica de la capacidad antioxidante de Synsepalum dulcificum : un análisis comparativo(Universidad EAFIT, 2024) Patiño Gallego, José Alejandro; Blandón Naranjo, Lucas Hernán; Quintero Quiroz, JuliánÍtem Aproximación metodológica para el desarrollo de modelos paleoclimáticos utilizando anatomía de maderas Neotropicales(Universidad EAFIT, 2022) Vásquez Rincón, Victor Manuel; Martínez Aguillón, CamilaÍtem Articulo de datos : escarabajos coprófagos, hormigas, abejas y mariposas del jardín botánico de Medellín (Antioquia, Colombia)(Universidad EAFIT, 2018) Arrieta Echeverri, Maria clara; Bedoya Escobar, AlejandraIn this study, the entomofauna of the Botanical Garden of Medellin was characterized, for which 4 groups were selected: coprophagous beetles (Coleoptera: Scarabaeidae), diurnal butterflies (Lepidoptera: Rhopalocera), ants (Hymenoptera: Formicidae) and bees (Hymenoptera: Apiodea). Insects were collected between January 30 and February 5, 2018. Four sampling stations were established. The selected methodologies correspond to pitfall traps with copro and necro bait, Van Someren Rydon traps, manual capture through free routes with time restriction. The dataset contains 454 records distributed as 41 records of dung beetles, 127 records of ants, 72 records of butterflies and 214 records of bees. The specimens were deposited at the Francisco Luis Gallego Entomological Museum. The resource contains information on 3 species of coprophagous beetles, 16 genera of antes, 23 species/morphospecies of bees and 23 species/morphospecies of diurnal butterflies. To date, there was no information on the insect’s richness of the Botanical Garden; so, these results are considered the first georeferenced records of these taxonomic groups in the urban area of Medellin and provide the baseline for the conservation strategy of insects in this important urban ecosystem.Ítem Asociaciones entre ofiuras y octocorales de ecosistemas de profundidad del Caribe colombiano, con nuevos registros para la región(Universidad EAFIT, 2020) Moreno Jiménez, Laura Cristina; Mejía Quintero, Katherine; Borrero Pérez, Giomar HelenaÍtem Cambios en el uso del suelo y su incidencia en la concentración y deposición de nitrogenados en el modelo de química de transporte LOTOS-EUROS para el Valle de Aburrá(Universidad EAFIT, 2021) Sánchez Aguirre, Andrés Felipe; Zapata Correa, Juliana; Yarce Botero, Andrés; López Restrepo, SantiagoConservation in the new century presents a multitude of challenges that are growing. One of the main challenges is the change in land use because it generates many transformed spaces that are constantly expanding and negatively affect the atmosphere, climate, ecosystems, and human health. Consideration in the land cover or use changes, together with transport chemistry models, enables the analysis of posible causes and consequences of future atmospheric dynamics in various temporal or spatial scenarios. The objective is to evaluate the variables of NOy Dry Deposition and NOx Surface Concentration, predicted by the LOTOS-EUROS transport chemistry model, when subjected to different land-use scenarios of the Aburrá Valley, generating an approximation to evaluate and predict the consequences of cover changes on the atmospheric dynamics of nitrogen and its possible effect on the surrounding ecosystems. This study analyzes different perspectives from the implementation for a case study in the Aburrá Valley of a chemistry-transport models as an important urban planning tool.Ítem Capacidad metabólica de cepas aisladas de la microbiota intestinal de mujeres gestantes y en período de lactancia para asimilar fuentes nutricionales ricas en colina : hacia el desarrollo de bioterapéuticos microbianos funcionales(Universidad EAFIT, 2022) Blel Jubiz, Shadia María; Quintero Rojas, María Isabel; Gómez Mesa, Laura; Sierra Zapata, LauraÍtem Captura de material particulado 1, 2.5 y 10 micras, con el uso de microalgas en fotobiorreactores exteriores(Universidad EAFIT, 2020) Mazo Zapata, Santiago; Miranda Parra, Alejandra María; Miranda Parra, Alejandra MaríaParticulate matter (PM), made up of a variety of organic and inorganic substances, is found to be harmful to human health. Due to its small size, this combination of substances can enter the body by the respiratory route and cause all kinds of related diseases, such as lung cancer, trachea, esophagus. In addition to this, the 1 micron particles can enter the circulatory system and cause associated problems such as stroke and cardiovascular damage. As a result of this, especially in the mainly affected cities, such as Medellín, they will come looking for methods to deal with this problem, suggesting the use of cutting-edge technology to try to largely solve PM contamination, however, the contributions that are They have made an impact on closed spaces, without identifying that they can be very expensive to maintain, on the other hand, a level outside the city or in outdoor conditions. The use of technology has been very low, and containment measures translate into social and mobility strategies such as spike and plate extension. In this study, the use of technology in outdoor conditions is proposed, through the use of photobioreactors and microalgae to evaluate the capture potential that these microorganisms have in the capture of this PM and how it is possible to extrapolate it as a future solution to use it in The city of Medellín. As a conclusion of the experiment, it was found that the Scenedesmus Obliquus ATCC 457 microalgae (S. Obliquss ATCC 457) could grow up to 1.52 g / L and retain up to 61.07% of PM 1, 65.45% of PM 2.5 and 87.01% of PM 10.Ítem Características respiratorias en el hongo fitopatógeno Colletotrichum acutatum eahp-008 : reserva bioenergética y actividad de la oxidasa alternativa(Universidad EAFIT, 2018) Arboleda Restrepo, Luisa María; Gómez Ramírez,Luis AlejandroÍtem Caracterización de criterios de muestreo de Megaptera novaeangliae en el hemisferio sur americano para la elección del modelado poblacional(Universidad EAFIT, 2024) Londoño Gallego, Juan Andrés; Catatño López, AlexandraThis paper focuses on characterizing the sampling criteria in population studies of humpback whales (Megaptera novaeangliae) in the American Southern Hemisphere and Southern Ocean, examining key aspects such as sampling periods, type of modeling, sampling employed, geographic region, and potential impacts on population study areas such as migration, abundance, and population estimation; with the objective of understanding the current status of the study of the species and identifying factors that influence population modeling and the establishment of appropriate management and conservation measures. The methodology included a systematic literature review following the PRISMA 2020 protocol, searching Scopus and PubMed and applying inclusion and exclusion criteria to select 67 articles published between 2010 and 2023. A matrix was constructed with bibliographic information characterizing each study according to type of sampling, temporal factor, modeling, geography and impact, identifying the most frequent data in each section. Finally, a decision tree was constructed based on the information in the matrix, suggesting the most appropriate type of model according to the data and methodology used in the population study, acting as a practical guide to facilitate the choice of the appropriate population modeling, considering sampling criteria and other relevant factors identified in the systematic review.