Biología (trabajo de grado)
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Ítem Estudio de vigilancia tecnológica e inteligencia competitiva en el mercado de células madre(Universidad EAFIT, 2017) López Zapata, Laura; Ramalho Almeida, Agostinho Joao; Méndez Naranjo, Katia CeciliaÍtem Análisis del genoma de influenza aviar H7N3 de la Epizootia 2012-2015 en México(Universidad EAFIT, 2017) Figueroa Varela, Paula Andrea; Martínez Barnetche, JesúsÍtem Determinación y evaluación de la microbiota intestinal común a la población colombiana(Universidad EAFIT, 2017) Valderrama Maya, Margarita María; Escobar Restrepo, Juan Sebastián; Pinel Peláez, NicolásÍtem Efecto del fuego en la dinámica del carbono de turberas de alta montaña en el Valle del Cóndor, PNN nevados, Colombia(Universidad EAFIT, 2018) Ospina Gómez, Laura Cristina; Benavides Duque, Juan CarlosÍtem Plukenetia volubilis non-paternal oDNA transmission : a cytological diagnosis of pollen grains and a phylogenetic approach assessing Colombian cultivars cpDNA markers variation(Universidad EAFIT, 2018) García Botero, Camilo Alejandro; Garcés Cardona, Froilán de Jesús; Correa Álvarez, Javier; Ramírez, VivianaÍtem Características respiratorias en el hongo fitopatógeno Colletotrichum acutatum eahp-008 : reserva bioenergética y actividad de la oxidasa alternativa(Universidad EAFIT, 2018) Arboleda Restrepo, Luisa María; Gómez Ramírez,Luis AlejandroÍtem Determinación de parámetros cinéticos de Synechococcus sp. pcc 7002 en cultivo sumergido(2018) Colorado Hernández, Salomé; Gómez Ramírez,Luis AlejandroÍtem Molecular identification of genetically modified castor bean (Ricinus communis) plants : first approximation(Universidad EAFIT, 2018) Hernández Tabares, Juliana; Villanueva Mejía, Diego; Ramírez Ríos, VivianaA genetic transformation protocol mediated by Agrobacteriumtumefaciens was carried out in castor bean epicotyls (Ricinus communis) by researchers from CIBIOP research group (Biological Sciences and Bioprocesses) at Universidad EAFIT. Ricinuscommunisseedlings were infected with strain LBA4404, which contained the binary vector pCAMBIA1305.2 which in turn had the genetic cassette CAM 35S-fatty acid hydroxylase (FAH12) - NOS terminator. For the transformed plants a selection medium was establis hed with respective concentrations of antibiotics such as hygromycin and cefotaxime. Seeking to verify the presence of inserts in genetically modified plants, DNA wasextractedfromthenewleaves from transformed epicotyl, for further analysis by conventional PCR. For DNA analysis, two tests were carried out, in which the PCRs of thehptII and theopt-FAH12 genes were standardized for their subsequent monitoring. Finally, as a confirmatory test, the obtained amplicons were sequenced by Sanger. The previously developed protocolisa relevant step to achieve a solid molecular characterization path looking forward to get a high efficient method to transform a tropical variety of Ricinus.Ítem Descripción inicial del ecosistema termo-ácido, el hidrotermal Aguas Calientes, proveniente de la cámara magmática del Nevado del Ruiz y de las comunidades que lo habitan(Universidad EAFIT, 2018) Corrales Cabra, Erika Margarita; Pinel Peláez, NicolásÍtem Articulo de datos : escarabajos coprófagos, hormigas, abejas y mariposas del jardín botánico de Medellín (Antioquia, Colombia)(Universidad EAFIT, 2018) Arrieta Echeverri, Maria clara; Bedoya Escobar, AlejandraIn this study, the entomofauna of the Botanical Garden of Medellin was characterized, for which 4 groups were selected: coprophagous beetles (Coleoptera: Scarabaeidae), diurnal butterflies (Lepidoptera: Rhopalocera), ants (Hymenoptera: Formicidae) and bees (Hymenoptera: Apiodea). Insects were collected between January 30 and February 5, 2018. Four sampling stations were established. The selected methodologies correspond to pitfall traps with copro and necro bait, Van Someren Rydon traps, manual capture through free routes with time restriction. The dataset contains 454 records distributed as 41 records of dung beetles, 127 records of ants, 72 records of butterflies and 214 records of bees. The specimens were deposited at the Francisco Luis Gallego Entomological Museum. The resource contains information on 3 species of coprophagous beetles, 16 genera of antes, 23 species/morphospecies of bees and 23 species/morphospecies of diurnal butterflies. To date, there was no information on the insect’s richness of the Botanical Garden; so, these results are considered the first georeferenced records of these taxonomic groups in the urban area of Medellin and provide the baseline for the conservation strategy of insects in this important urban ecosystem.Ítem Evaluación in vitro de la actividad antiparasitaria de las especies vegetales Uncaria tomentosa, Picramnia spruceana y Solanum nigrum sobre leishmaniasis(Universidad EAFIT, 2018) Córdoba Fuzga, Angélica María; Sáez Vega, Alex Armando; Robledo Restrepo, Sara MaríaÍtem Clasificación de especies arbóreas según su capacidad para remover material particulado del aire en el Valle de Aburrá(Universidad EAFIT, 2018) Vásquez Bedoya, Manuela; Arroyave Maya, María del PilarAir pollution is a global problem and Colombia is not immune to this problem. Specifically, the Aburrá Valley has presented high levels of particulate matter in the air (PM) that exceed the limits established by the Colombian Air Quality Standard and by the World Health Organization (WHO). In this work, a ranking of the 100 most common trees species in Valle de Aburrá that better control the PM was made, based on its functional traits. Retrophyllum rospigliosii, Inga densiflora, Zygia longifolia, Bauhinia picta and Calliandra haematocephala were the five native species with the greatest capacity for PM removal. We suggested that those species that are above the average of 17.8 in the ranking be considered in planting plans aimed at improving the air quality within the Aburrá Valley.Ítem Application of sensitivity- and uncertainty-based techniques for the assessment of epidemiological models in real-life study cases(Universidad EAFIT, 2019) Rojas Díaz, Daniel; Vélez Sánchez, Carlos Mario; Puerta Yepes, María EugeniaUncertainty analysis (UA) and sensitivity analysis (SA) are tools to assess and to quantify the uncertainty spread from the input factors (parameters and initial states) to the model output, taking into account the effect of the interactions among those factors. Throughout the following works, I treat UA as a graphical assessment of uncertainty propagation based on Monte Carlo simulation, which makes it possible to state a range for the model output in cases where it is considered relevant. On the other hand, I privilege the global approach for SA instead of the local one, since the first attempts to quantify the uncertainty contribution of the model factors in their entire distribution range while the second one is only informative for a single locus in the distribution. In this way, when applying global UA/SA on a model, it is possible to identify those factors that mostly determine the model behavior. Furthermore, I have noticed that the concepts and principles of UA/SA are associated with other main tasks in modeling, as factors estimation and confidence intervals achievement: Briefly, those non-identifiable factors in a model (factors whose value can not be estimated uniquely from some information about output data) should belong to the categories of non-sensible or sensitive but correlated from SA; and, the sub-space of the space of factors where the factors may jointly exist producing a model output that fits, in some extent, to a given output data, could be approximately estimated with UA-based approaches, constituting a new kind of confidence interval. Thus, in this compendium, I present five works related to the applications of UA/SA techniques as well as its relevance. The objective of those applications evolves from the most logically immediate to some derived and more complex ones, though still preserving the model pertinence as a central topic.Ítem Inferring tumor age from multiple neutral evolutionary process(Universidad EAFIT, 2019) Londoño Muñoz, Susana; Pulido Tamayo, SergioBackground: All cancer arise as a result of a somatic mutation, several models have been developed to understand cancer, it has been seen as an evolutionary process in which somatic mutations are the pieces of the puzzle. Somatic mutation catalogue of the cancer genome has been used to reconstruct cancer phylogeny, in this case somatic mutations without selective pressure will help determine the age of a tumor. Results: Any type of mutation can have an impact on the cell, the majority of them are neutral, mutations that have less functional impact, that means less selective pressure on the tumor evolution are the ones used as temporal measurement of the tumors. A positive correlation was found between two types of measurements, which shows that an appropriate age can be extracted using both of them. Conclusion: The use of this method can be coupled with other cancer analysis, for example, along with co-occurrence and mutual exclusivity analysis could bring a better understanding of the tumor, an appropriate tumor classification and with this a more accurate clinical decision, which leads to an efective treatment.Ítem Actividad anti-leishmania in vitro de los extractos naturales obtenidos de Crescentia Cujete y Piper Aduncum(Universidad EAFIT, 2019) Posada Bermúdez, Angélica; Robledo Restrepo, Sara MaríaThe present study aimed to measure in vitro anti-Leishmania activity of leaf extract in water, leaves plus stems in water and leaves in ethanol obtained from Crescentia cujete and Piper aduncum, to achieve this, cytotoxicity was calculated which for all extracts it was high (LC50: <100 μg/mL). The anti-Leishmania activity was also determined and even though values are in the high activity range (EC50: <25 μg/mL), by taking into account low results of selectivity index (SI), it is possible to affirm that it is due to cell death, hence of parasites, although their death is not because of extracts. In conclusion, extracts present more cytotoxicity than activityÍtem Diversidad y predicción del potencial funcional de las comunidades bacterianas asociadas a vitroplantas de banano cv. Williams(Universidad EAFIT, 2019) Isaza Álvarez, María Camila; Correa Álvarez, Javier; García Giraldo, GisellÍtem Cloning the human pro-apoptotic BAX gene into a high-expression yeast vector designed for overexpressing genes in Yeast 2.0.(Universidad EAFIT, 2019) Montoya Londoño, Daniel; Sáez Vega, Alex ArmandoÍtem Phylogenomics of the plant family Orchidaceae : solving phylogenetic ambiguities within Cymbidieae and Orchidoideae(Universidad EAFIT, 2019) Serna Sánchez, Maria Alejandra; Alvarez Yelac, Astrid Catalina; Arcila, Juliana; Arias, Tatiana; Arias Garzón, TatianaÍtem Patrones de ocupación y actividad de los roedores Simpátricos Cuniculus Paca y Dasyprocta Punctata en áreas protegidas del norte de la Cordillera Central, Colombia(Universidad EAFIT, 2019) Giraldo Amaya, Mateo; Díaz Nieto, Juan Fernando; Sánchez Giraldo, CamiloÍtem Evaluación de las cepas Bacillus subtilis EA-CB0015 y Bacillus amyloliquefaciens EA-CB0959 sobre cuatro hongos patógenos de arroz(Universidad EAFIT, 2019) Valencia Riascos, Luisa María; Arroyave Toro, Juan José; Villegas Escobar, Valeska