Artículos (CIBIOP)

URI permanente para esta colección

https://hdl.handle.net/10784/5095

Examinar

Mostrando 1 - 20 de 72

Dextran causes aggregation of mitochondria and influences their oxidoreductase activities and light scattering
(ELSEVIER SCIENCE INC, 2003-01-01) Lemeshko, Victor; López, Luis Fernando; Solano González, Sigifredo; Rendón Rivera, Dairo Alonso; Gómez, Luis A.; Universidad EAFIT. Departamento de Ciencias; Ciencias Biológicas y Bioprocesos (CIBIOP)
It has been reported that dextrans diminish the intermembrane space of mitochondria, increase the number of contact sites between the inner and the outer mitochondrial membranes, decrease the outer membrane permeability to adenosine 5(')-diphosphate, and
A possible restriction of ferro- and ferricyanide oxidoreductase activities of rat liver mitochondria by the outer membrane.
(ELSEVIER SCIENCE INC, 2005-11-15) Ramirez, LAG; Lemeshko, VV; Universidad EAFIT. Departamento de Ciencias; Ciencias Biológicas y Bioprocesos (CIBIOP)
In this work, various ferro-ferricyanide oxidoreductase activities of rat liver mitochondria were studied to find conditions under which the outer membrane might restrict the flux of these highly charged non-biological anions. When the isotonic low ionic strength medium was supplemented with 25mM KCl, a several-fold increase in the succinate-ferricyanide reductase activity of mitochondria and in the rate of external NADH oxidation in the presence of ferrocyanide was observed. Mitochondrial respiration with 5mM ferrocyanide was almost completely inhibited after consumption of 3.8-18.5% of the dissolved oxygen, depending on the medium and the presence of 2,4-dinitrophenol. These and other experimental data together with mathematical modeling of the redox-state equilibrium suggest that the measured activities might be restricted by two factors: first, the permeability of the outer mitochondrial membrane and second, a strong influence of the ionic strength of incubation media on the intermembrane space redox reactions.
Experimentos en el aula de clase para enseñanza de la física
(Sociedad Colombiana de Física, 2006-01-01) J. I. Marulanda; Gómez, Luis A.; Universidad EAFIT. Departamento de Ciencias; Ciencias Biológicas y Bioprocesos (CIBIOP)
Considerando la importancia de los aspectos fenomenológicos como motivación para elaborar modelos formales en la enseñanza de la física, la experimentación se convierte en un recurso didáctico valioso en este proceso.
Supercomplexes of the mitochondrial electron transport chain decline in the aging rat heart
(ELSEVIER SCIENCE INC, 2009-10-01) Gómez, Luis A.; Monette, Jeffrey S.; Chavez, Juan D.; Maier, Claudia S.; Hagen, Tory M.; Universidad EAFIT. Departamento de Ciencias; Ciencias Biológicas y Bioprocesos (CIBIOP)
Accumulation of mitochondrial electron transport chain (ETC) defects is a recognized hallmark of the age-associated decline in cardiac bioenergetics; however, the molecular events involved are only poorly understood. In the present work,
Characteristics of the Rat Cardiac Sphingolipid Pool in Two Mitochondrial Subpopulations
(Elsevier Inc., 2010-06-01) Monette, Jeffrey S.; Gómez, Luis A.; Moreau, Regis; Taylor, Alan; Hagen, Tory M.; Universidad EAFIT. Departamento de Ciencias; Ciencias Biológicas y Bioprocesos (CIBIOP)
Mitochondrial sphingolipids play a diverse role in normal cardiac function and diseases, yet a precise quantification of cardiac mitochondrial sphingolipids has never been performed. Therefore, rat heart interfibrillary mitochondria (IFM) and subsarcolemm
(R)-alpha-Lipoic acid treatment restores ceramide balance in aging rat cardiac mitochondria
(Elsevier Inc., 2011-01-01) Monette, Jeffrey S.; Gómez, Luis A.; Moreau, Regis; Dunn, Kevin C.; Butler, Judy A.; Finlay, Liam A.; Michels, Alexander J.; Petersen-Shay, Kate; Smith, Eric J.; Hagen, Tory M.; Universidad EAFIT. Departamento de Ciencias; Ciencias Biológicas y Bioprocesos (CIBIOP)
Inflammation results in heightened mitochondrial ceramide levels, which cause electron transport chain dysfunction, elevates reactive oxygen species, and increases apoptosis. As mitochondria in aged hearts also display many of these characteristics, we hy
Acetyl-L-carnitine supplementation reverses the age-related decline in carnitine palmitoyltransferase 1 (CPT1) activity in interfibrillar mitochondria without changing the L-carnitine content in the rat heart
(Elsevier BV, 2012-02-01) Gómez, Luis A.; Du Heath, Shi-Hua; Hagen, Tory M.; Universidad EAFIT. Departamento de Ciencias; Ciencias Biológicas y Bioprocesos (CIBIOP)
The aging heart displays a loss of bioenergetic reserve capacity partially mediated through lower fatty acid utilization. We investigated whether the age-related impairment of cardiac fatty acid catabolism occurs, at least partially, through diminished le
Age-related decline in mitochondrial bioenergetics: Does supercomplex destabilization determine lower oxidative capacity and higher superoxide production
(Copyright, 2012-09-01) Gómez, Luis A.; Hagen, Tory M.; Universidad EAFIT. Departamento de Ciencias; Ciencias Biológicas y Bioprocesos (CIBIOP)
Mitochondrial decay plays a central role in the aging process. Although certainly multifactorial in nature, defective operation of the electron transport chain (ETC) constitutes a key mechanism involved in the age-associated loss of mitochondrial energy m
Scale-up from shake flasks to pilot-scale production of the plant growth-promoting bacterium Azospirillum brasilense for preparing a liquid inoculant formulation
(Springer Verlag, 2013) Villegas, Jesús; Trujillo Roldán, Mauricio Alberto; Blancas Cabrera, Abel; Gamboa Suasnavart, Ramsés Adolfo; García Cabrera, Ramsés I.; Acevedo Sánchez, Eduardo V.; Valdez Cruz, Norma Adriana; González Monterrubio, César Fernando; Martínez Salinas, Carlos; Marín Palacio, Luz Deisy; Unidad de Bioprocesos, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México; Biofábrica Siglo XXI S.A. de C.V., Carretera México-Oaxaca Km 106, Col; Universidad EAFIT. Departamento de Ciencias; Mauricio A. Trujillo Roldán (maurotru@biomedicas.unam.mx); Ciencias Biológicas y Bioprocesos (CIBIOP)
Azospirillum brasilense has industrial significance as a growth promoter in plants of commercial interest -- However, there is no report in the literature disclosing a liquid product produced in pilot-scale bioreactors and is able to be stored at room temperature for more than 2 years -- The aim of this work was to scale up a process from a shake flask to a 10- L lab-scale and 1,000-L pilot-scale bioreactor for the production of plant growth-promoting bacterium A -- brasilense for a liquid inoculant formulation -- Furthermore, this work aimed to determine the shelf life of the liquid formulation stored at room temperature and to increase maize crops yield in greenhouses -- Under a constant oxygen mass transfer coefficient (KLa), a fermentation process was successfully scaled up from shake flasks to 10- and 1,000-L bioreactors -- A concentration ranging from 3.5 to 7.5×108 CFU/mL was obtained in shake flasks and bioreactors, and after 2 years stored at room temperature, the liquid formulation showed one order of magnitude decrease -- Applications of the cultured bacteria in maize yields resulted in increases of up to 95 % in corncobs and 70 % in aboveground biomass
Fengycin C produced by Bacillus subtilis EA-CB0015.
(AMER CHEMICAL SOC, 2013-04-26) Villegas-Escobar, Valeska; Ceballos, Isabel; Mira, John J.; Edith Argel, Luz; Orduz Peralta, Sergio; Romero-Tabarez, Magally; Universidad EAFIT. Departamento de Ciencias; Ciencias Biológicas y Bioprocesos (CIBIOP)
Bacillus subtilis EA-CB0015 was isolated from the phyllosphere of a banana plant and tested for its potential to produce bioactive compounds against Mycosphaerella fijiensis. Using a dual plate culture technique the cell-free supernatant of B. subtilis EA-CB0015 produced inhibition values of 89 +/- 1%. The active compounds were purified by solid-phase extraction and HPLC, and their primary structures determined using mass spectrometry and amino acid analysis. A new fengycin isoform, fengycin C, with the amino acid sequence Glu-Orn-Tyr-Thr-Glu-Val-Pro-Gln-Thr-Ile was isolated. The peptidic moiety differs from fengycin B at position 9 and from fengycin A at positions 6 and 9. The beta-hydroxy fatty acyl chain is connected to the N-terminal of the decapeptide and can be saturated or unsaturated, ranging from 14 to 18 carbons. The C-terminal residue of the peptidic moiety is linked to the tyrosine residue at position 3, forming the branching point of the acyl peptide and the eight-membered cyclic lactone.
Scale-up from shake flasks to bioreactor, based on power input and Streptomyces lividans morphology, for the production of recombinant APA (45/47 kDa protein) from Mycobacterium tuberculosis.
(Kluwer Academic Publishers, 2013-08-01) Gamboa-Suasnavart RA; Marín-Palacio LD; Martínez-Sotelo JA; Espitia C; Servín-González L; Valdez-Cruz NA; Trujillo-Roldán MA; Universidad EAFIT. Departamento de Ciencias; Ciencias Biológicas y Bioprocesos (CIBIOP)
Culture conditions in shake flasks affect filamentous Streptomyces lividans morphology, as well the productivity and O-mannosylation of recombinant Ala-Pro-rich O-glycoprotein (known as the 45/47 kDa or APA antigen) from Mycobacterium tuberculosis. In order to scale up from previous reported shake flasks to bioreactor, data from the literature on the effect of agitation on morphology of Streptomyces strains were used to obtain gassed volumetric power input values that can be used to obtain a morphology of S. lividans in bioreactor similar to the morphology previously reported in coiled/baffled shake flasks by our group. Morphology of S. lividans was successfully scaled-up, obtaining similar mycelial sizes in both scales with diameters of 0.21 ± 0.09 mm in baffled and coiled shake flasks, and 0.15 ± 0.01 mm in the bioreactor. Moreover, the specific growth rate was successfully scaled up (0.09 ± 0.02 and 0.12 ± 0.01 h(-1), for bioreactors and flasks, respectively), and the recombinant protein productivity measured by densitometry, as well. More interestingly, the quality of the recombinant glycoprotein measured as the amount of mannoses attached to the C-terminal of APA was also scaled- up; with up to five mannose residues in cultures carried out in shake flasks; and six in the bioreactor. However, final biomass concentration was not similar, indicating that although the process can be scaled-up using the power input, others factors like oxygen transfer rate, tip speed or energy dissipation/circulation function can be an influence on bacterial metabolism.
Tecia solanivora, Povolny (Lepidoptera: Gelechiidae): una revisión sobre su origen, dispersión y estrategias de control biológico
(Fondo Editorial Universidad EAFIT, 2013-09-26) D.F. Villanueva-Mejía; Saldamando, Clara Inés; Universidad EAFIT. Departamento de Ciencias; Ciencias Biológicas y Bioprocesos (CIBIOP)
In 1956, Tecia solanivora was reported by the first time in Central America. This Lepidopteran insect of the Gelechiidae family was found to severely damage potato crops (Solanum tuberosum), which adversely affected local economy...
Scale-up from shake flasks to pilot-scale production of the plant growth-promoting bacterium Azospirillum brasilense for preparing a liquid inoculant formulation
(SPRINGER, 2013-11-01) Trujillo-Roldan, Mauricio A.; Valdez-Cruz, Norma A.; Gonzalez-Monterrubio, Cesar F.; Acevedo-Sanchez, Eduardo V.; Martinez-Salinas, Carlos; Garcia-Cabrera, Ramses I.; Gamboa-Suasnavart, Ramses A.; Marin-Palacio, Luz D.; Villegas, Jesus; Blancas-Cabrera, Abel; Universidad EAFIT. Departamento de Ciencias; Ciencias Biológicas y Bioprocesos (CIBIOP)
Azospirillum brasilense has industrial significance as a growth promoter in plants of commercial interest. However, there is no report in the literature disclosing a liquid product produced in pilot-scale bioreactors and is able to be stored at room temperature for more than 2 years. The aim of this work was to scale up a process from a shake flask to a 10-L lab-scale and 1,000-L pilot-scale bioreactor for the production of plant growth-promoting bacterium A. brasilense for a liquid inoculant formulation. Furthermore, this work aimed to determine the shelf life of the liquid formulation stored at room temperature and to increase maize crops yield in greenhouses. Under a constant oxygen mass transfer coefficient (K L a), a fermentation process was successfully scaled up from shake flasks to 10- and 1,000-L bioreactors. A concentration ranging from 3.5 to 7.5 × 108 CFU/mL was obtained in shake flasks and bioreactors, and after 2 years stored at room temperature, the liquid formulation showed one order of magnitude decrease. Applications of the cultured bacteria in maize yields resulted in increases of up to 95 % in corncobs and 70 % in aboveground biomass. © 2013 Springer-Verlag Berlin Heidelberg.
The role of volumetric power input in the growth, morphology, and production of a recombinant glycoprotein by Streptomyces lividans in shake flasks
(Elsevier, 2014) Gamboa Suasnavart, Ramsés A; Servín González, Luis; Soto, Enrique; Klöckner, Wolf; Büchs, Jochen; Trujillo Roldán, Mauricio A; Marín Palacio, Luz Deisy; Valdez Cruz, Norma Adriana; Córdova Aguilar, María Soledad; Unidad de Bioprocesos, Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México; Centro de Ciencias Aplicadas y Desarrollo Tecnológico, Universidad Nacional Autónoma de México; AVT–Biochemical Engineering, RWTH Aachen University; Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México; Universidad EAFIT. Departamento de Ciencias; Mauricio A TrujilloRoldán (maurotru@gmail.com); Ciencias Biológicas y Bioprocesos (CIBIOP)
The impact of flask geometry on Streptomyces lividans growth and morphology, production and Omannosylation of a recombinant O-glycoprotein (APA from Mycobacterium tuberculosis) was described and associated to the evolution of the volumetric power input (P/V) in three shake flask geometries -- During the exponential growth, the highest P/V was found in baffled flasks (BF) with 0.51 kW/m3, followed by coiled flasks (CF) with 0.44 kW/m3 and normal Erlenmeyer flasks (NF) with 0.20 kW/m3 (flasks volume of 250 mL, filling with 50 mL and agitated at 150 rpm) -- During the stationary phase, P/V decreased 20% in BF and CF, but increased two times in NF, surely due to changes in mycelial morphology and its effects on rheology -- Also, NF cultures were carried out at a filling volume and agitation of 15 mL, 150 rpm (15 mL-NF), and 25 mL, 168 rpm (25 mL-NF), in order to raise P/V closely to the values obtained in CF -- However, different growth, morphology and recombinant protein productivity were obtained -- These data indicate that P/V is not a definitive parameter that can determine bacteria growth and morphology, not even glycoprotein production -- But it can be proposed that the oxygen transfer in the center of the pellets and hydromechanical stress might be the more relevant parameters than P/V
Multiple response optimization of Bacillus subtilis EA-CB0015 culture and identification of antifungal metabolites
(Biocatalysis and Agricultural Biotechnology, 2014) Ordúz, Sergio; Mosquera, Sandra; González Jaramillo, Lina María; Villegas Escobar, Valeska; Escuela de Biociencias; Centro de Investigaciones del Banano; Universidad EAFIT. Departamento de Ciencias; Valeska Villegas-Escobar (vvilleg2@eafit.edu.co); Ciencias Biológicas y Bioprocesos (CIBIOP)
The low yields of biomass and antimicrobial metabolites obtained in fermentation processes are limiting factors for implementing biological control agents in the field -- In this context, optimization of the culture medium for the biological control agent Bacillus subtilis EA-CB0015 was conducted in submerged culture to maximize the biomass production and antifungal activity -- Additionally, the active metabolites against the phytopathogen Mycosphaerella fijiensis produced under optimized conditions were identified -- Carbon and nitrogen constituents of the culture medium were optimized using a 22 factorial design with central point followed by a multiple response optimization coupled to the desirability function approach -- The optimized medium (33.4 g/L of glucose and 32.5 g/L of yeast extract), showed significant increases in both, cell density by 3.6 folds (13.271.0 g/L) and antifungal activity by 1.2 folds (77.770.3% inhibition) -- To gain insight into the type of active compounds, they were purified and identified by mass spectrometry (MS) -- The MS analysis revealed the presence of three families of lipopeptides: surfactin, fengycin and iturin -- The high antifungal activity was associated with the novel fengycin C and with iturin -- A which were partially growth-associated with a maximum concentration of 781.4 and 355.4 mg/L at 36 h of growth, respectively -- These results indicate the potential of B. subtilis EA-CB0015 to produce high concentrations of biomass and lipopeptides that can be exploited for biotechnological application
Fengycin C produced by Bacillus subtilis EA-CB0015
(American Chemical Society, 2014) Ceballos, Isabel; J. Mira, John; Argel, Luz Edith; Orduz Peralta, Sergio; Romero Tabarez, Magally; Villegas Escobar, Valeska; Centro de Investigaciones del Banano; Instituto de Biotecnología, Universidad Nacional de Colombia, Bogotá; Universidad Nacional de Colombia, Medellín; Universidad EAFIT. Departamento de Ciencias; Valeska Villegas Escobar ( vvilleg2@eafit.edu.co.); Ciencias Biológicas y Bioprocesos (CIBIOP)
Bacillus subtilis EA-CB0015 was isolated from the phyllosphere of a banana plant and tested for its potential to produce bioactive compounds against Mycosphaerella fijiensis -- Using a dual plate culture technique the cell-free supernatant of B. subtilis EA-CB0015 produced inhibition values of 89 ± 1% -- The active compounds were purified by solid-phase extraction and HPLC, and their primary structures determined using mass spectrometry and amino acid analysis -- A new fengycin isoform, fengycin C, with the amino acid sequence Glu-Orn-Tyr-Thr-Glu-Val-Pro-Gln-Thr-Ile was isolated -- The peptidic moiety differs from fengycin B at position 9 and from fengycin A at positions 6 and 9 -- The β-hydroxy fatty acyl chain is connected to the N-terminal of the decapeptide and can be saturated or unsaturated, ranging from 14 to 18 carbons -- The C-terminal residue of the peptidic moiety is linked to the tyrosine residue at position 3, forming the branching point of the acyl peptide and the eight-membered cyclic lactone
Evaluation of Antimalarial and Cytotoxic Activity of Styrylquinolines obtained from 8-Hydroxyquinaldine with Aromatic Aldehydes
(Centro de Información Tecnológica - CIT, 2014) Torres Ayazo, Omar Leonardo; Espinosa Sáez, Roger David; Álex Armando, Sáez Vega; Santafé Patiño, Gilmar Gabriel,; Universidad de Córdoba. Grupo de Investigación Desarrollo de Fármacos y Afines IDEFARMA; Universidad de Córdoba. Grupo de Química de los Productos Naturales; Universidad EAFIT. Departamento de Ciencias; Torres, Omar L. (omart2365@gmail.com); Espinosa, Roger D. (rdespinosa03@gmail.com); Saez, Alex A.(asaez70@gmail.com); Santafé, Gilmar G. ( gsantafe@correo.unicordoba.edu.co); Ciencias Biológicas y Bioprocesos (CIBIOP)
This study reports the synthesis of five (E)-Styrylquinolines (Q.1, Q.2, Q.3, Q.5, Q.6) and three O-alkylated derivatives (Q.1a, Q.6a, Q 6 b) and the evaluation of its antimalarial activity and cytotoxic in vitro -- For Styrylquinolines Perkin condensation reaction type from 8-hydroxyquinaldine with aromatic aldehydes was used -- Antimalarial activity was performed using the method Radioisotope with FCB-2 strains of Plasmodium falciparum (chloroquine-resistant) and for the cytotoxic activity the MTT method with HepG2 cells was employed -- Most compounds showed to be active antimalarial -- Those with higher activity were Q.3 (IC506.4μM) and Q.5 (IC5010.8 µM), although all of them were cytotoxic at concentrations above 1 µg / mL -- The structures of these compounds were confirmed using infrared spectroscopy techniques and MRI in one and two dimensions
Biology stage of Moniliophthora roreri in Colombia
(Universidad Nacional de Colombia, 2014) Correa Álvarez, Javier; Castro Martínez, Sergio; Coy, Jairo; Universidad EAFIT. Departamento de Ciencias; Javier Correa Álvarez (jcorre38@eafit.edu.co); Ciencias Biológicas y Bioprocesos (CIBIOP)
Frosty pod rot disease of cocoa plants is caused by the basiodiomycete Moniliophthora roreri -- Nowadays, this disease is present in almost all Latin American countries producers of cocoa beans, exhibiting high adaptation to diverse environments -- In Colombia, it is the most important disease attacking cocoa crops and the traditional strategies for control have generated side results as strengthening in genetic resistance of strains in some regions -- In this review, we collected the most relevant biological and agricultural aspects of this disease such as origin and distribution of the disease, life cycle, forms of disease control and research projects oriented to understand the genetic and evolution of this pathogen -- Finally, we suggest increasing the number of basic researches, aiming to understand, how this pathogen has evolved its genome in different habitats, favoring its genetic variation -- Thus, with all this knowledge, we could advance in biotechnology programs for control and prevention of the Frosty rod pot
Estado de la moniliasis del cacao causada por Moniliophthora roreri en Colombia
(Universidad Nacional de Colombia, 2014-01-01) Álvarez, J.C.; Martínez, S.C.; Coy, J.; Universidad EAFIT. Departamento de Ciencias; Ciencias Biológicas y Bioprocesos (CIBIOP)
Frosty pod rot disease of cocoa plants is caused by the basiodiomycete Moniliophthora roreri. Nowadays, this disease is present in almost all Latin American countries producers of cocoa beans, exhibiting high adaptation to diverse environments. In Colombia, it is the most important disease attacking cocoa crops and the traditional strategies for control have generated side results as strengthening in genetic resistance of strains in some regions. In this review, we collected the most relevant biological and agricultural aspects of this disease such as origin and distribution of the disease, life cycle, forms of disease control and research projects oriented to understand the genetic and evolution of this pathogen. Finally, we suggest increasing the number of basic researches, aiming to understand, how this pathogen has evolved its genome in different habitats, favoring its genetic variation. Thus, with all this knowledge, we could advance in biotechnology programs for control and prevention of the Frosty rod pot.
Evaluación de la actividad antimalárica y citotóxica de estirilquinolinas obtenidas a partir de 8-hidroxiquinaldina con aldehídos aromáticos
(Centro de Informacion Tecnologica, 2014-01-01) Torres, O.L.; Espinosa, R.D.; Saez, A.A.; Santafé, G.G.; Universidad EAFIT. Departamento de Ciencias; Ciencias Biológicas y Bioprocesos (CIBIOP)
This study reports the synthesis of five (E)-Styrylquinolines (Q.1, Q.2, Q.3, Q.5, Q.6) and three O-alkylated derivatives (Q.1a, Q.6a, Q 6 b) and the evaluation of its antimalarial activity and cytotoxic in vitro. For Styrylquinolines Perkin condensation reaction type from 8-hydroxyquinaldine with aromatic aldehydes was used. Antimalarial activity was performed using the method Radioisotope with FCB-2 strains of Plasmodium falciparum (chloroquine-resistant) and for the cytotoxic activity the MTT method with HepG2 cells was employed. Most compounds showed to be active antimalarial. Those with higher activity were Q.3 (IC50 6.4µM) and Q.5 (IC50 10.8 µM), although all of them were cytotoxic at concentrations above 1 µg / mL. The structures of these compounds were confirmed using infrared spectroscopy techniques and MRI in one and two dimensions. © 2014, Centro de Informacion Tecnologica. All rigths reserved.

Examinar

Examinando Artículos (CIBIOP) por Fecha de publicación

Resultados por página

Opciones de ordenación