Biología (trabajo de grado)
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Ítem Genomic insights into Pannus brasiliensis CCIBt3594 a freshwater Cyanobacterium from Pantanal, Brazil(Universidad EAFIT, 2023) Betancurt Botero, Natalia; de Fátima Fiore, Marli; Junior Machado, MauricioÍtem Caracterización de la respuesta de Moniliophthora roreri a fungicidas químicos : eficacia y potencial de generar resistencia(Universidad EAFIT, 2023) Serna Muriel, Daniela; Mosquera López, Sandra; Correa Álvarez, JavierÍtem Type locality clarification and redescription of Andinobates opisthomelas (Boulenger, 1899)(Universidad EAFIT, 2023) Durango Cardona, Juan Pablo; Arredondo Salgar, Juan CamiloÍtem Diskriminierung von infektiösen und nicht-infektiösen SARS-CoV-2 Viren mit Hilfe einer zu optimierenden Extraktionsmethode und unter qRT-PCR Anwendung und Verwendung von EMA und PMAxx(Universidad EAFIT, 2023) Sierra Peláez, Susana; Harms, CarstenÍtem Mitigation of Bovine Oocyte Aging Through NMN Supplementation During in vitro Maturation(Universidad EAFIT, 2023) Carrillo Gómez, Andrea Carolina; Torres Osorio, Viviana; Duque Rodríguez, Matteo; Urrego Álvarez, Rodrigo Antonio; Urrego Álvarez, Rodrigo Antonio; Torres Osorio, VivianaÍtem Clonación del gen crtI, procedente de Rhodotorula mucilaginosa EA-BIO010 en cepas de Escherichia coli TOP10, mediante técnicas de ingeniería genética(Universidad EAFIT, 2023) Ospina Mejía, Manuel; Sierra Zapata, Laura; Villanueva Mejía, Diego FernandoReactive oxygen species are unstable molecules that contain oxygen and react easily with other molecules in the cell where they cause damage to lipid molecules, proteins and DNA. To combat them, antioxidants such as beta-carotene have been identified, produced by organisms such as Blakeslea trispora, Dunalliela salina, Rhodotorula glutinis, Rhodotorula mucilaginosa, among others. However, beta-carotene is produced in low amounts compared to other antioxidants by yeast strains such as R. mucilaginosa. It should be noted that many of the methodologies used today for the extraction of antioxidants from plants or microorganisms are inefficient, expensive and environmentally unsustainable. Yeasts of the genus Rhodotorula then become promising candidates for beta-carotene production, since it has been shown that their beta-carotene producing cluster can be expressed heterologously in other organisms to optimize its production. Therefore, this project aimed to establish the first stage of the methodology for the heterologous production of the biosynthetic cluster of Rhodotorula mucilaginosa EA-BIO010 in E. coli TOP10 strains, starting with the cloning of the crtl gene. This new recombinant micro-organism could be used in the future for the sustainable and efficient production of beta-carotenes and contribute to the creation of biological processes for the production of antioxidants for industrial use.Ítem Evaluación de la riqueza taxonómica, riqueza funcional y variabilidad de gremios alimenticios de la avifauna presente en el Refugio de Vida Silvestre Aguas Frías, Medellín(Universidad EAFIT, 2023) Ramírez Valencia, Camila; Pinel Peláez, Nicolás; Cataño López, AlexandraÍtem Desarrollo de ruta de producción de prototipo de probiótico de Bacillus amyloliquefaciens(Universidad EAFIT, 2023) Pino Herrera, María José; Bardales Mendieta, JorgeÍtem Rastreando la Evolución de los Factores de Transcripción TALE : Implicaciones para la diferenciación celular y desarrollo(Universidad EAFIT, 2023) Barbosa Pérez, Andrés; Sánchez Rodríguez, AminaelÍtem Ensayos de germinación y modelo de distribución para Godoya Antioquiensis en Antioquia y regiones aledañas(Universidad EAFIT, 2023) Botero López, Juan José; Martínez Aguillón, Camila; Restrepo Cossío, Leydi ValentinaGodoya antioquiensis is a tree species native to Colombia, distributed throughout the central and western mountain range, between 1600 m above sea level (masl) and 2600 masl. Currently, due to anthropogenic factors and overpopulation that have altered environmental conditions, its populations are under threat. The objective of the present study is to generate data and tools regarding its propagation and potential distribution to promote the conservation of the species. To determine seed viability, a tetrazolium staining test was conducted, revealing a 10% variation in viability between stored and fresh seeds, as well as greater imbibition for the latter. According to the germination results, the best substrate in terms of the quantity of seedlings obtained in the initial phase (30 days) is sand. However, despite Sphagnum presenting a lower result, its seedlings showed better development. Additionally, it is expected that the distribution model obtained will identify potential occurrence zones where the species has not been previously recorded. Moreover, based on the results of the imbibition test, Godoya antioquiensis seeds can withstand prolonged periods of desiccation. However, storing them is not advisable as they may lose germinative vigor. Due to the wide distribution of the species, in the Antioquia department, there is good availability of optimal Andean ecosystems for its reintroduction.Ítem Evaluación del proceso de reintroducción de tres manatíes antillanos (Trichechus manatus manatus) liberados en República Dominicana mediante la caracterización de su dieta(Universidad Eafit, 2023) Villegas Molina, Vanessa; Álvarez Alemán, Anmari; Martínez Aguillón, CamilaÍtem Measuring the Agreement Between two Dengue Virus Diagnostic Tests in Samples from Endemic Regions of Colombia(Universidad EAFIT, 2023) Rodríguez Lopera, Manuela; Ciuoderis, Karl A.Ítem Polen colectado por Tetragonisca angustula (Apidae, Meliponini) en espacios urbanos de Medellín, Colombia(Universidad EAFIT, 2023) Alzate Hincapie, Brian Steven; Martínez Aguillón, CamilaMeliponine bees play an essential role in the pollination of plants. In particular, Tetragonisca angustula is common in urban and semi-urban environments in Colombia. This species collects pollen from various plant families according to the availability of resources. This study compares the diversity of pollen collected by T. angustula in hives at two locations in Medellin: Universidad EAFIT and the Jardín Botánico “Joaquín Antonio Uribe” de Medellín. The results reveal the significant influence of certain plant families in the diet of these bees, highlighting Bignoniaceae, Fabaceae, and Asteraceae at EAFIT, and Moraceae, Myrtaceae, and Arecaceae at the Jardín Botánico de Medellín. This understanding is crucial for their conservation and the promotion of biodiversity in urban environments, emphasizing the fundamental role of bees in pollination and how their sustainable management can contribute to their preservation in urban settings and the conservation of biodiversity in cities.Ítem Caracterización genómica y morfológica de bacteriófagos en Bacillus tequilensis EA-CB0015(Universidad EAFIT, 2023) Gómez Estrada, Pablo; García Botero, Camilo; Villegas Escobar, ValeskaÍtem Four new species of mygalomorph spiders (Araneae, Halonoproctidae and Theraphosidae) from the Colombian Pacific region (Bahía Solano, Chocó)(Universidad EAFIT, 2023) Echeverri Díez, Mariana; Gómez Torres, Sebastián; Pinel Peláez, Nicolás; Perafán Liévano, Carlos; Perafán Liévano, Carlos; Pinel Peláez, NicolásÍtem Patrones de actividad de mamíferos medianos y grandes en un paisaje transformado de la región del Magdalena Medio, Antioquia, Colombia(Universidad EAFIT, 2023) Álvarez Arias, Juan David; Sánchez Giraldo, CamiloÍtem Inventario y evaluación del potencial ornamental de la familia Gesneriaceae en el Cañón del río Melcocho (El Carmen de Viboral, Antioquia, Colombia)(Universidad EAFIT, 2023) Peláez Vélez, Samuel; Tuberquia Muñóz, Dino Jesús; Martínez Aguillón, CamilaThe use of native plants in urban green spaces can be beneficial for local wildlife and climate resilience in cities. Despite its large plant biodiversity, there are few native species used for ornamental purposes in Colombia. Gesneriaceae is a family of plants recognized for its great ornamental potential which is highly diverse in Colombia. This study aimed to build an inventory of the Gesneriad species present in the Melcocho river basin, El Carmen de Viboral, Antioquia, and to develop and apply a method of asessment of these species’ ornamental potential. 23 species, distributed in 13 genera were found, 9 being new records for the locality. An asessment system was developed using nine criteria based in morphologic and environmental ornamental attributes, with input from six experts in the field. Eight species were valued as having a high ornamental potential, twelve were classified as having average potential and three as having a low potential. This method was succesful in prioritizing species for future ornamental and landscaping use and can be adapted to other plant groups with similar versatile habits.Ítem Posibles consecuencias de los contaminantes orgánicos ambientales en los cetáceos de los océanos de Colombia : revisión sistemática(Universidad EAFIT, 2023) Tobón Toro, Maria Camila; Pinel Peláez, NicolásÍtem Identificación y caracterización de isoformas de la enzima desaturasa de ácidos grasos 2 (fad2) en ricinus communis mediante métodos moleculares y computacionales - primera fase(Universidad EAFIT, 2023) Vélez Zapata, Jhon Alexander; Villanueva Mejía, Diego Fernando; Susunaga Gómez, Danna MelissaÍtem Evaluating different computational routines for the assembly of mitochondrial genomes using long reads from Oxford Nanopore(Universidad EAFIT, 2022) Corrales Orozco, Mariana; Díaz Nieto, Juan Fernando