Ciencias Biológicas y Bioprocesos (CIBIOP)

URI permanente para esta comunidad

https://hdl.handle.net/10784/5094

Busca desarrollar tecnologías biológicas competitivas y diseñar bioprocesos económicamente viables, a fin de contribuir con nuevas estrategias que impacten la producción sostenible de alimento y el mejoramiento de la salud humana.

Líneas de investigación:Bioprocesos; Sistemas Moleculares y Celulares.
Código Minciencias: COL0143589.
Categoría 2019: A1.
Escuela: Ciencias.
Departamento académico: Ciencias Biologicas.
Coordinador: Luis Alejandro Gómez Ramírez.
Correo electrónico: lgomez1@eafit.edu.co

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Mostrando 1 - 20 de 90

Acetyl-L-carnitine supplementation reverses the age-related decline in carnitine palmitoyltransferase 1 (CPT1) activity in interfibrillar mitochondria without changing the L-carnitine content in the rat heart
(Elsevier BV, 2012-02-01) Gómez, Luis A.; Du Heath, Shi-Hua; Hagen, Tory M.; Universidad EAFIT. Departamento de Ciencias; Ciencias Biológicas y Bioprocesos (CIBIOP)
The aging heart displays a loss of bioenergetic reserve capacity partially mediated through lower fatty acid utilization. We investigated whether the age-related impairment of cardiac fatty acid catabolism occurs, at least partially, through diminished le
Age-related decline in mitochondrial bioenergetics: Does supercomplex destabilization determine lower oxidative capacity and higher superoxide production
(Copyright, 2012-09-01) Gómez, Luis A.; Hagen, Tory M.; Universidad EAFIT. Departamento de Ciencias; Ciencias Biológicas y Bioprocesos (CIBIOP)
Mitochondrial decay plays a central role in the aging process. Although certainly multifactorial in nature, defective operation of the electron transport chain (ETC) constitutes a key mechanism involved in the age-associated loss of mitochondrial energy m
Antimycotic activity of fengycin C biosurfactant and its interaction with phosphatidylcholine model membranes
(ELSEVIER SCIENCE BV, 2017-08-01) González-Jaramillo LM; Aranda FJ; Teruel JA; Villegas-Escobar V; Ortiz A; Universidad EAFIT. Departamento de Ciencias; Ciencias Biológicas y Bioprocesos (CIBIOP)
Lipopeptide biosurfactants constitute one of the most promising groups of compounds for the treatment and prevention of fungal diseases in plants. Bacillus subtilis strain EA-CB0015 produces iturin A, fengycin C and surfactin and it has been proven useful for the treatment of black Sigatoka disease in banana plants, an important pathology caused by the fungus Mycosphaerella fijiensis (Morelet). We have found that B. subtilis EA-CB0015 cell free supernatants and purified fractions inhibit M. fijiensis cellular growth. The effect of the purified lipopeptides mentioned above on fungal growth has been also evaluated, observing that iturin A and fengycin C inhibit mycelial growth and ascospore germination, whereas surfactin is not effective. On the hypothesis that the antifungal action of the lipopeptides is associated to their incorporation into biological membranes, ultimately leading to membrane permeabilization, a detailed biophysical study on the interaction of a new isoform of fengycin C with model dipalmitoyphosphatidylcholine (DPPC) membranes has been carried out. Differential scanning calorimetry shows that fengycin C alters the thermotropic phase transitions of DPPC, and is laterally segregated in the fluid bilayer forming domains. Fluorescent probe polarization measurements show that fengycin C does not affect the hydrophobic interior of the membrane. This latter perturbation is concomitant with a strong dehydration of the polar region of DPPC, as shown by FTIR. Fengycin-rich domains, where the surrounding DPPC molecules are highly dehydrated, may well constitute sites of membrane permeabilization leading to a leaky target membrane. These results are a solid support to explain the membrane perturbing action of fengycin, which has been related to its antifungal activity. (C) 2017 Elsevier B.V. All rights reserved.
Aplicaciones del pululano en la conservación de alimentos y bioempaques: vigilancia tecnológica e inteligencia competitiva
(Universidad Nacional de Colombia, 2016-09-21) Catalina Giraldo E; Posada, Juan Pablo; Bustamante, Ana Maria; Universidad EAFIT. Departamento de Ciencias; Ciencias Biológicas y Bioprocesos (CIBIOP)
Se presenta el ejercicio de Vigilancia Tecnológica e Inteligencia Competitiva (VT&IC) para las aplicaciones del polisacárido pululano en el campo de los alimentos y empaques
Bacillus subtilis EA-CB0575 genome reveals clues for plant growth promotion and potential for sustainable agriculture
(Springer, 2020-01-01) Franco-Sierra, N.D.; Posada, L.F.; Santa-María, G.; Romero-Tabarez, M.; Villegas-Escobar, V.; Álvarez, J.C.; Universidad EAFIT. Departamento de Ciencias; Ciencias Biológicas y Bioprocesos (CIBIOP)
Bacillus subtilis is a remarkably diverse bacterial species that displays many ecological functions. Given its genomic diversity, the strain Bacillus subtilis EA-CB0575, isolated from the rhizosphere of a banana plant, was sequenced and assembled to determine the genomic potential associated with its plant growth promotion potential. The genome was sequenced by Illumina technology and assembled using Velvet 1.2.10, resulting in a whole genome of 4.09 Mb with 4332 genes. Genes involved in the production of indoles, siderophores, lipopeptides, volatile compounds, phytase, bacilibactin, and nitrogenase were predicted by gene annotation or by metabolic pathway prediction by RAST. These potential traits were determined using in vitro biochemical tests, finding that B. subtilis EA-CB0575 produces two families of lipopeptides (surfactin and fengycin), solubilizes phosphate, fixes nitrogen, and produces indole and siderophores compounds. Finally, strain EA-CB0575 increased 34.60% the total dry weight (TDW) of tomato plants with respect to non-inoculated plants at greenhouse level. These results suggest that the identification of strain-specific genes and predicted metabolic pathways might explain the strain potential to promote plant growth by several mechanisms of action, accelerating the development of plant biostimulants for sustainable agricultural. © 2020, Springer-Verlag GmbH Germany, part of Springer Nature.
Biocontrol activity of Bacillus subtilis EA-CB0015 cells and lipopeptides against postharvest fungal pathogens
(Academic Press Inc., 2017-11-01) Arroyave-Toro J.J.; Mosquera S.; Villegas-Escobar V.; Universidad EAFIT. Departamento de Ciencias; Ciencias Biológicas y Bioprocesos (CIBIOP)
Post-harvest diseases are responsible for significant losses worldwide, especially the plant pathogenic fungi Botrytis cinerea and Colletotrichum sp. are particularly severe and devastating. In this study, nine fungal pathogens were screened for growth inhibition by Bacillus subtilis EA-CB0015 strain and its metabolites. In vitro inhibitory assays showed that B. subtilis EA-CB0015 cells and the cell free supernatant (CFS) inhibited the growth of the tested fungal pathogens with different susceptibilities. Therefore, the antifungal activities of lipopeptides iturin A and fengycin C contained in the CFS, were tested against C. acutatum and B. cinerea. C. acutatum was more susceptible with minimal inhibitory concentrations (MIC) of 32 ppm (iturin A) and 128 ppm (fengycin C). Fruit and flower trials confirmed that B. subtilis EA-CB0015 cells and its lipopeptides reduced postharvest disease development but to differing degrees. Anthracnose symptoms caused by C. acutatum in tamarillo fruits were completely abolished by CFS, iturin A and fengycin C and reduced by 76% when treated with B. subtilis cells. In contrast, grey mold disease symptoms caused by B. cinerea in chrysanthemum flowers were inhibited by 72% when treated with lipopeptides and by 39% when applied with B. subtilis EA-CB0015 cells. Our results indicate that lipopeptides and cells of B. subtilis EA-CB0015 have a broad antifungal spectrum and control postharvest diseases caused by susceptible fungal pathogens. Our findings open the possibility of incorporating this biological control agent into different disease management programs.
Biodegradation of vegetable residues by polygalacturonase-agar using a trickle-bed bioreactor
(Institution of Chemical Engineers, 2018-09-01) Ramírez-Tapias, Y.A.; Rivero, C.W.; Giraldo-Estrada, C.; Britos, C.N.; Trelles, J.A.; Universidad EAFIT. Departamento de Ciencias; Ciencias Biológicas y Bioprocesos (CIBIOP)
Bacterial pectinases degrade the pectic substances present in plant tissues and particularly, polygalacturonases catalyze the hydrolysis of a-(1,4) glycosidic bonds linking D-galacturonic acid units. In this study, polygalacturonase from Streptomyces halstedii ATCC 10897 was immobilized by the matrix entrapment technique using different thermogels. Bacteriological agar added with magnesium cation produced beads with a more stabilized microstructure for enzyme retention, monitored by oscillatory measurements of storage and loss modulus. Agar concentration and protein content were optimized to maximize protein entrapment, product conversion, and reaction yield. Results showed that the mixture at 10:90% (v/v) of protein (2 mg/mL) and agar (4% w/v) was the best immobilization condition to retain 91% of protein and hydrolyze 38% of pectin to allow the highest reaction yield (9.279 g/g) and increase stability up to 48 h of successive reactions. Agarose bead biocatalysts were used in a trickle-bed column operated with recirculation, and this bioreactor allowed the degradation of pear and cucumber residues by enzymatic liquefaction to enhance sugar content up to 15.33 and 9.35 mg/mL, respectively, and decrease viscosity by 92.3%. The scale-up of this process adds value to vegetable residues such as fructooligosaccharides or fermentable sugars, which become a sustainable source of fuels and chemicals. © 2018 Institution of Chemical Engineers
Biodegradation of vegetable residues by polygalacturonase-agar using a trickle-bed bioreactor. En: Food and Bioproducts Processing
(Institution of Chemical Engineers, 2018-09-01) GIRALDO, CATALINA; Universidad EAFIT. Departamento de Ciencias; Ciencias Biológicas y Bioprocesos (CIBIOP)
Biology stage of Moniliophthora roreri in Colombia
(Universidad Nacional de Colombia, 2014) Correa Álvarez, Javier; Castro Martínez, Sergio; Coy, Jairo; Universidad EAFIT. Departamento de Ciencias; Javier Correa Álvarez (jcorre38@eafit.edu.co); Ciencias Biológicas y Bioprocesos (CIBIOP)
Frosty pod rot disease of cocoa plants is caused by the basiodiomycete Moniliophthora roreri -- Nowadays, this disease is present in almost all Latin American countries producers of cocoa beans, exhibiting high adaptation to diverse environments -- In Colombia, it is the most important disease attacking cocoa crops and the traditional strategies for control have generated side results as strengthening in genetic resistance of strains in some regions -- In this review, we collected the most relevant biological and agricultural aspects of this disease such as origin and distribution of the disease, life cycle, forms of disease control and research projects oriented to understand the genetic and evolution of this pathogen -- Finally, we suggest increasing the number of basic researches, aiming to understand, how this pathogen has evolved its genome in different habitats, favoring its genetic variation -- Thus, with all this knowledge, we could advance in biotechnology programs for control and prevention of the Frosty rod pot
Bioprospecting of aerobic endospore-forming bacteria with biotechnological potential for growth promotion of banana plants
(Elsevier, 2016-11-22) Posada, L.F.; Ramírez, M.; Ochoa-Gómez, N.; Cuellar-Gaviria, T.Z.; Argel-Roldan, L.E.; Ramírez, C.A.; Villegas-Escobar, V.; Universidad EAFIT. Departamento de Ciencias; Ciencias Biológicas y Bioprocesos (CIBIOP)
High amounts of agrochemicals are regularly used for increasing yields in cultivation of banana. The use of plant growth-promoting rhizobacteria (PGPR) could represent an environmentally friendly alternative that can improve productivity. In this study, we tested a funnel-like strategy to bioprospect aerobic endospore-forming bacteria (AEFB) that could be useful to develop a biotechnological product to promote the growth of banana plants. First, 837 aerobic endospore-forming bacteria were obtained from the rhizosphere of banana and plantain. Then, the isolates were assessed for both: their capacity to promote growth of maize seedlings (used as a model plant) and to display specific biochemical PGPR-associated traits. Twenty-two of these strains significantly increased the dry weight of maize seedlings, some of them showing in vitro PGPR traits. Based on this screening, four isolates were selected to conduct evaluations on banana plants, from which, the bacterial strain Bacillus subtilis EA-CB0575 was chosen as a promising plant growth -promoting isolate. Further studies with this strain showed that the application of either spores, vegetative cells (both at concentrations 1 x 10(7) and 1 x 10(8) CFU/mL), or the cell-free supernatant (CFS) of its fermentation significantly increased the dry weight of banana plants, compared with the non-treated control. Our results suggest that both cellular structures of B. subtilis EA-CB0575 and the metabolites and/or elements contained in its CFS enhance the growth and development of banana plants. (C) 2016 Elsevier B.V. All rights reserved.
Booklet Use of Antioquian propolis. Quality criteria and extraction protocols
(Universidad EAFIT, 2021) Marín Palacio, Luz Deisy; Builes Toro, Santiago; Barrientos Lezcano, Juan Camilo; Universidad EAFIT
This booklet is aimed at beekeepers in the Antioquia region, and aims to improve the processes of collecting and extracting propolis in beekeeping communities.
Ceratocystis cacaofunesta genome analysis reveals a large expansion of extracellular phosphatidylinositol-specific phospholipase-C genes (PI-PLC)
(BioMed Central Ltd., 2018-01-17) Molano, E.P.L.; Cabrera, O.G.; Jose, J.; do Nascimento, L.C.; Carazzolle, M.F.; Teixeira, P.J.P.L.; Alvarez, J.C.; Tiburcio, R.A.; Tokimatu Filho, P.M.; de Lima, G.M.A.; Guido, R.V.C.; Corrêa, T.L.R.; Leme, A.F.P.; Mieczkowski, P.; Pereira, G.A.G.; Universidad EAFIT. Departamento de Ciencias; Ciencias Biológicas y Bioprocesos (CIBIOP)
Background: The Ceratocystis genus harbors a large number of phytopathogenic fungi that cause xylem parenchyma degradation and vascular destruction on a broad range of economically important plants. Ceratocystis cacaofunesta is a necrotrophic fungus responsible for lethal wilt disease in cacao. The aim of this work is to analyze the genome of C. cacaofunesta through a comparative approach with genomes of other Sordariomycetes in order to better understand the molecular basis of pathogenicity in the Ceratocystis genus. Results: We present an analysis of the C. cacaofunesta genome focusing on secreted proteins that might constitute pathogenicity factors. Comparative genome analyses among five Ceratocystidaceae species and 23 other Sordariomycetes fungi showed a strong reduction in gene content of the Ceratocystis genus. However, some gene families displayed a remarkable expansion, in particular, the Phosphatidylinositol specific phospholipases-C (PI-PLC) family. Also, evolutionary rate calculations suggest that the evolution process of this family was guided by positive selection. Interestingly, among the 82 PI-PLCs genes identified in the C. cacaofunesta genome, 70 genes encoding extracellular PI-PLCs are grouped in eight small scaffolds surrounded by transposon fragments and scars that could be involved in the rapid evolution of the PI-PLC family. Experimental secretome using LC-MS/MS validated 24% (86 proteins) of the total predicted secretome (342 proteins), including four PI-PLCs and other important pathogenicity factors. Conclusion: Analysis of the Ceratocystis cacaofunesta genome provides evidence that PI-PLCs may play a role in pathogenicity. Subsequent functional studies will be aimed at evaluating this hypothesis. The observed genetic arsenals, together with the analysis of the PI-PLC family shown in this work, reveal significant differences in the Ceratocystis genome compared to the classical vascular fungi, Verticillium and Fusarium. Altogether, our analyses provide new insights into the evolution and the molecular basis of plant pathogenicity. © 2018 The Author(s).
Ceratocystis wilt pathogens: History and biology-highlighting C. Cacaofunesta, the causal agent of wilt disease of cacao
(Springer International Publishing, 2016-01-01) Cabrera, O.G.; Molano, E.P.L.; José, J.; Álvarez, J.C.; Pereira, G.A.G.
Ceratocystis is a genus of ascomycete fungi that includes aggressive pathogens of economically important plants worldwide. This fungus is the causal agent of Ceratocystis wilt disease and canker disease, which often kills the plant causing major losses in agricultural production. In the last two decades, emerging diseases related to Ceratocystis infections have been greatly increased. Ceratocystis wilt of cacao is caused by C. cacaofunesta, one of the three well-established host-specific pathogens in the genus. Ceratocystis wilt of cacao has caused sporadic epidemics in the Americas, but its importance is often underestimated. Furthermore, the disease represents a serious threat to the world’s cacao production due to the risk of pathogen spread. Silvicultural practices in cacao agroforests, the marketing of seeds, and cacao grafting in association with a minimal knowledge of the biology of the pathogen effectively contributed to this threat. This chapter explores the controversial taxonomic and evolutionary history of the genus Ceratocystis as well as the biology of C. cacaofunesta. © Springer International Publishing Switzerland 2016.
Ceratocystis Wilt Pathogens: History and Biology—Highlighting C. cacaofunesta, the Causal Agent of Wilt Disease of Cacao
(Springer International Publishing, 2016-01-01) García, Oladys; López, Eddy Patricia; José, Juliana; Álvarez, J.C.
Characteristics of the Rat Cardiac Sphingolipid Pool in Two Mitochondrial Subpopulations
(Elsevier Inc., 2010-06-01) Monette, Jeffrey S.; Gómez, Luis A.; Moreau, Regis; Taylor, Alan; Hagen, Tory M.; Universidad EAFIT. Departamento de Ciencias; Ciencias Biológicas y Bioprocesos (CIBIOP)
Mitochondrial sphingolipids play a diverse role in normal cardiac function and diseases, yet a precise quantification of cardiac mitochondrial sphingolipids has never been performed. Therefore, rat heart interfibrillary mitochondria (IFM) and subsarcolemm
Colombia's cyberinfrastructure for biodiversity: Building data infrastructure in emerging countries to foster socioeconomic growth
(2019-12-22) De Vega, Jose J.; Davey, Robert P.; Duitama, Jorge; Escobar, Dairo; Cristancho, Marco A.; Etherington, Graham J.; Minotto, Alice; Pineda J.D.; Correa Alvarez J; Camargo, Anyela V.; Haerty, Wilfried; Mallarino, Juan P.; Barreto, Emiliano; Fuentes, Narcis; Di, Federica; Universidad EAFIT. Departamento de Ciencias; Ciencias Biológicas y Bioprocesos (CIBIOP)
Science and innovation are not a luxury but a prerequisite for social and economic development (Annan, 2003).
Colombian genetically modified potato lines resistant to Tecia solanivora (Lepidoptera: Gelechiidae) under a confined field
(Sociedad Colombiana de Entomologia, 2014-07-01) Villanueva, Diego; Torres, Javier; Rivera, Hugo; Nunez, Victor; Arango, Rafael; Angel, Fernando; Universidad EAFIT. Departamento de Ciencias; Ciencias Biológicas y Bioprocesos (CIBIOP)
Tecia solanivora is one of the most damaging pest of potato crops in Central and South America. Eight genetically modified (GM) potato lines with Bacillus thuringiensis cry1Ac gene (Bt potato lines) were characterized at molecular, immunological and biological level under biosafety confined, greenhouse and experimental field conditions. Additionally, preliminary assays regarding substantial equivalence were determined. Cry1Ac protein and gene were detected and quantified on leaf and tubers. DC 40.5, DC 40.7A, DC 40.7B, PP 28, PP 28.1 and PP 40.A, Bt potato lines were resistant to Tecia solanivora when they were exposed. Results obtained indicated that two GM potato lines are promising for further assessments and selection tests as well as future possible commercialization.
¿Cómo transformar los recursos naturales?
(2016-05-13) Alvarez, A.; Giraldo, C.; Londoño, A.; González, A.
Complete mitogenome of the biocontroller fungus Purpureocillium sp. (Ascomycota, Ophiocordycipitaceae, Hypocreales)
(Taylor and Francis Ltd., 2018-01-01) Cardona N.L.; Franco-Sierra N.D.; Correa Alvarez J.; Universidad EAFIT. Departamento de Ciencias; Ciencias Biológicas y Bioprocesos (CIBIOP)
The strain Purpureocillium sp. UdeA0106 is an antagonist of nematodes, fungi, and garden symphylans from crops with high economic importance in Colombia (Salazar 2013; Salazar et al. 2014; Cardona et al. 2014; Gallego et al. 2014) and is being studied to be proposed as new species. It was included on the 1000 fungal genomes project to elucidate its phylogenetic relationships with other fungi. Purpureocillium’s mitogenome has 23,495 bp of circular size. It contains 15 protein-coding genes without duplications (PCGs), corresponding to the 60% of its total length, 23 transfer genes (7.6% tRNA), two of them duplicated (trnR and trnM), and two ribosomal genes (17.6% rRNA) and a GC content of 28.44%. A phylogenetic tree was proposed using their 14 PCGs mitochondrial genes and was compared with other fungi of the Subphylum Pezizomycotina. Phylogenetics relationships showed UdeA0106 to be close to P. chlamydosporia and M. anisopliae forming a cluster with other fungal biocontrol agents and separated the strain of plant pathogenic fungi. © 2018, © 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
Construction of probe of the plant growth-promoting bacteria Bacillus subtilis useful for fluorescence in situ hybridization
(Elsevier, 2016-09-01) Posada, L.F.; Alvarez, J.C.; Hu, C.-H.; de-Bashan, L.E.; Bashan, Y.; Universidad EAFIT. Departamento de Ciencias; Ciencias Biológicas y Bioprocesos (CIBIOP)
Strains of Bacillus subtilis are plant growth-promoting bacteria (PGPB) of many crops and are used as inoculants. PGPB colonization is an important trait for success of a PGPB on plants. A specific probe, based on the 16 s rRNA of Bacillus subtilis, was designed and evaluated to distinguishing, by fluorescence in situ hybridization (FISH), between this species and the closely related Bacillus amyloliquefaciens. The selected target for the probe was between nucleotides 465 and 483 of the gene, where three different nucleotides can be identified. The designed probe successfully hybridized with several strains of Bacillus subtilis, but failed to hybridize not only with B. amyloliquefaciens, but also with other strains such as Bacillus altitudinis, Bacillus cereus, Bacillus gibsonii, Bacillus megaterium, Bacillus pumilus; and with the external phylogenetic strains Azospirillum brasilense Cd, Micrococcus sp. and Paenibacillus sp. The results showed the specificity of this molecular probe for B. subtilis. (C) 2016 Elsevier B.V. All rights reserved.

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