Molecular identification of genetically modified castor bean (Ricinus communis) plants : first approximation

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dc.contributor.advisorVillanueva Mejía, Diegospa
dc.contributor.advisorRamírez Ríos, Vivianaspa
dc.contributor.authorHernández Tabares, Juliana
dc.coverage.spatialMedellín de: Lat: 06 15 00 N degrees minutes Lat: 6.2500 decimal degrees Long: 075 36 00 W degrees minutes Long: -75.6000 decimal degreeseng
dc.creator.degreeBiólogo(a)spa
dc.creator.emailjherna27@eafit.edu.cospa
dc.date.accessioned2019-06-14T19:46:49Z
dc.date.available2019-06-14T19:46:49Z
dc.date.issued2018
dc.description.abstractA genetic transformation protocol mediated by ​Agrobacteriumtumefaciens was carried out in castor bean epicotyls (​Ricinus communis) by researchers from CIBIOP research group (Biological Sciences and Bioprocesses) at Universidad EAFIT. ​Ricinuscommunisseedlings were infected with strain LBA4404, which contained the binary vector pCAMBIA1305.2 which in turn had the genetic cassette ​CAM 35S-fatty acid hydroxylase (FAH12) - NOS terminator. For the transformed plants a selection medium was establis hed with respective concentrations of antibiotics such as hygromycin and cefotaxime. Seeking to verify the presence of inserts in genetically modified plants, DNA wasextractedfromthenewleaves from transformed epicotyl, for further analysis by conventional PCR. For DNA analysis, two tests were carried out, in which the PCRs of the​hptII and the​opt-FAH12 genes were standardized for their subsequent monitoring. Finally, as a confirmatory test, the obtained amplicons were sequenced by Sanger. The previously developed protocolisa relevant step to achieve a solid molecular characterization path looking forward to get a high efficient method to transform a tropical variety of ​Ricinus.spa
dc.formatapplication/pdfeng
dc.identifier.ddc581.35 H557
dc.identifier.urihttp://hdl.handle.net/10784/13614
dc.language.isospaspa
dc.publisherUniversidad EAFITspa
dc.publisher.departmentEscuela de Ciencias. Ciencias Básicasspa
dc.publisher.placeMedellínspa
dc.publisher.programBiologíaspa
dc.rights.accessrightsinfo:eu-repo/semantics/closedAccessspa
dc.rights.localAcceso cerradospa
dc.subject​​Ricinus communisspa
dc.subject.keyword​​Ricinus communisspa
dc.subject.keywordRicinoleic acidspa
dc.subject.keywordGMOspa
dc.subject.keywordPCRspa
dc.subject.keywordTransformation efficiencyspa
dc.subject.keywordOilseed plantspa
dc.subject.lembHUELLAS DE PLANTAS POR ADNspa
dc.subject.lembSEMILLAS OLEAGINOSASspa
dc.subject.lembGENÉTICA VEGETALspa
dc.subject.lembBIOLOGÍA MOLECULARspa
dc.titleMolecular identification of genetically modified castor bean (Ricinus communis) plants : first approximationspa
dc.typeinfo:eu-repo/semantics/bachelorThesiseng
dc.typebachelorThesiseng
dc.type.hasVersionacceptedVersioneng
dc.type.localTrabajo de gradospa

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