An improved HPLC-DAD method for clavulanic acid quantification in fermentation broths of Streptomyces clavuligerus

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dc.citation.journalTitleJOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSISspa
dc.contributor.authorRamirez-Malule H
dc.contributor.authorJunne S
dc.contributor.authorLópez C
dc.contributor.authorZapata J
dc.contributor.authorSáez A
dc.contributor.authorNeubauer P
dc.contributor.authorRios-Estepa R
dc.contributor.departmentUniversidad EAFIT. Departamento de Cienciasspa
dc.contributor.researchgroupCiencias Biológicas y Bioprocesos (CIBIOP)spa
dc.date.accessioned2021-03-23T20:08:55Z
dc.date.available2021-03-23T20:08:55Z
dc.date.issued2016-02-20
dc.description.abstractClavulanic acid (CA) is an important secondary metabolite commercially produced by cultivation of Streptomyces clavuligerus (Sc). It is a potent inhibitor of bacterial beta-lactamases. In this work, a specific and improved high performance liquid chromatography (HPLC) method, using a C-18 reversed phase column, diode array detector and gradient elution for CA quantification in fermentation broths of Sc, was developed and successfully validated. Samples were imidazole-derivatized for the purpose of creating a stable chromophore (clavulanate-imidazole). The calibration curve was linear over a typical range of CA concentration between 0.2 and 400 mg/L. The detection and quantification limits were 0.01 and 0.02 mg/L, respectively. The precision of the method was evaluated for CA spiked into production media and a recovery of 103.8%, on average, was obtained. The clavulanate-imidazole complex was not stable when the samples were not cooled during the analysis. The recovery rate was 39.3% on average. This assay was successfully tested for CA quantification in samples from Sc fermentation, using both, a chemically defined and a complex medium. (C) 2015 Elsevier B.V. All rights reserved.eng
dc.identifierhttps://eafit.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=1638
dc.identifier.doi10.1016/j.jpba.2015.12.035spa
dc.identifier.issn07317085spa
dc.identifier.issn1873264Xspa
dc.identifier.otherSCOPUS;2-s2.0-84942813761
dc.identifier.urihttp://hdl.handle.net/10784/26792
dc.language.isoengeng
dc.publisherElsevier
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84952837330&doi=10.1016%2fj.jpba.2015.12.035&partnerID=40&md5=6a3e57dd4825fa3e52df175ccb6cea4a
dc.rightshttps://v2.sherpa.ac.uk/id/publication/issn/0731-7085
dc.sourceJOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
dc.subject.keywordClavulanic acideng
dc.subject.keywordStreptomyces clavuligeruseng
dc.subject.keywordHigh performance liquid chromatography methodeng
dc.subject.keywordFermentation processeseng
dc.subject.keywordClavulanate-imidazoleeng
dc.titleAn improved HPLC-DAD method for clavulanic acid quantification in fermentation broths of Streptomyces clavuligeruseng
dc.typearticleeng
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.typepublishedVersioneng
dc.type.localArtículo

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