A possible restriction of ferro- and ferricyanide oxidoreductase activities of rat liver mitochondria by the outer membrane.

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dc.citation.journalTitleARCHIVES OF BIOCHEMISTRY AND BIOPHYSICSspa
dc.contributor.authorRamirez, LAG
dc.contributor.authorLemeshko, VV
dc.contributor.departmentUniversidad EAFIT. Departamento de Cienciasspa
dc.contributor.researchgroupCiencias Biológicas y Bioprocesos (CIBIOP)spa
dc.date.accessioned2021-03-23T20:08:55Z
dc.date.available2021-03-23T20:08:55Z
dc.date.issued2005-11-15
dc.description.abstractIn this work, various ferro-ferricyanide oxidoreductase activities of rat liver mitochondria were studied to find conditions under which the outer membrane might restrict the flux of these highly charged non-biological anions. When the isotonic low ionic strength medium was supplemented with 25mM KCl, a several-fold increase in the succinate-ferricyanide reductase activity of mitochondria and in the rate of external NADH oxidation in the presence of ferrocyanide was observed. Mitochondrial respiration with 5mM ferrocyanide was almost completely inhibited after consumption of 3.8-18.5% of the dissolved oxygen, depending on the medium and the presence of 2,4-dinitrophenol. These and other experimental data together with mathematical modeling of the redox-state equilibrium suggest that the measured activities might be restricted by two factors: first, the permeability of the outer mitochondrial membrane and second, a strong influence of the ionic strength of incubation media on the intermembrane space redox reactions.eng
dc.identifierhttps://eafit.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=1484
dc.identifier.doi10.1016/j.abb.2005.08.019spa
dc.identifier.issn00039861spa
dc.identifier.issn10960384spa
dc.identifier.otherSCOPUS;2-s2.0-84911898065
dc.identifier.urihttp://hdl.handle.net/10784/26791
dc.language.isoengeng
dc.publisherELSEVIER SCIENCE INC
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-27644577029&doi=10.1016%2fj.abb.2005.08.019&partnerID=40&md5=bf343b65ed044f522be74bdb060c6be3
dc.rightshttps://v2.sherpa.ac.uk/id/publication/issn/0003-9861
dc.sourceARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
dc.subject.keyword2,4 dinitrophenoleng
dc.subject.keyworddissolved oxygeneng
dc.subject.keywordferricyanide oxidoreductaseeng
dc.subject.keywordferrocyanideeng
dc.subject.keywordferrocyanide oxidoreductaseeng
dc.subject.keywordoxidoreductaseeng
dc.subject.keywordpotassium chlorideeng
dc.subject.keywordreduced nicotinamide adenine dinucleotideeng
dc.subject.keywordsuccinate ferricyanide reductaseeng
dc.subject.keywordunclassified drugeng
dc.subject.keywordanimal tissueeng
dc.subject.keywordarticleeng
dc.subject.keywordcontrolled studyeng
dc.subject.keywordenzyme activityeng
dc.subject.keywordionic strengtheng
dc.subject.keywordliver mitochondrioneng
dc.subject.keywordmaleeng
dc.subject.keywordmathematical modeleng
dc.subject.keywordmitochondrial respirationeng
dc.subject.keywordnonhumaneng
dc.subject.keywordouter membraneeng
dc.subject.keywordoxidationeng
dc.subject.keywordoxidation reduction reactioneng
dc.subject.keywordpriority journaleng
dc.subject.keywordrateng
dc.subject.keywordAnimalseng
dc.subject.keywordCellseng
dc.subject.keywordCulturedeng
dc.subject.keywordEnzyme Activationeng
dc.subject.keywordFerricyanideseng
dc.subject.keywordFerrocyanideseng
dc.subject.keywordMaleeng
dc.subject.keywordMitochondriaeng
dc.subject.keywordLivereng
dc.subject.keywordMitochondrial Membraneseng
dc.subject.keywordModelseng
dc.subject.keywordBiologicaleng
dc.subject.keywordNADHeng
dc.subject.keywordNADPH Oxidoreductaseseng
dc.subject.keywordOxidoreductaseseng
dc.subject.keywordRatseng
dc.subject.keywordAnimaliaeng
dc.titleA possible restriction of ferro- and ferricyanide oxidoreductase activities of rat liver mitochondria by the outer membrane.eng
dc.typearticleeng
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.typepublishedVersioneng
dc.type.localArtículo

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