Multiple response optimization of Bacillus subtilis EA-CB0015 culture and identification of antifungal metabolites

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dc.citation.journalTitleBiocatalysis and Agricultural Biotechnologyspa
dc.contributor.authorMosquera, S.
dc.contributor.authorGonzález-Jaramillo, L.M.
dc.contributor.authorOrduz, S.
dc.contributor.authorVillegas-Escobar, V.
dc.contributor.departmentUniversidad EAFIT. Departamento de Cienciasspa
dc.contributor.researchgroupCiencias Biológicas y Bioprocesos (CIBIOP)spa
dc.date.accessioned2021-03-23T20:08:56Z
dc.date.available2021-03-23T20:08:56Z
dc.date.issued2014-01-01
dc.description.abstractThe low yields of biomass and antimicrobial metabolites obtained in fermentation processes are limiting factors for implementing biological control agents in the field. In this context, optimization of the culture medium for the biological control agent Bacillus subtilis EA-CB0015 was conducted in submerged culture to maximize the biomass production and antifungal activity. Additionally, the active metabolites against the phytopathogen Mycosphaerella fijiensis produced under optimized conditions were identified. Carbon and nitrogen constituents of the culture medium were optimized using a 22 factorial design with central point followed by a multiple response optimization coupled to the desirability function approach. The optimized medium (33.4g/L of glucose and 32.5g/L of yeast extract), showed significant increases in both, cell density by 3.6 folds (13.2±1.0g/L) and antifungal activity by 1.2 folds (77.7±0.3% inhibition). To gain insight into the type of active compounds, they were purified and identified by mass spectrometry (MS). The MS analysis revealed the presence of three families of lipopeptides: surfactin, fengycin and iturin. The high antifungal activity was associated with the novel fengycin C and with iturin A which were partially growth-associated with a maximum concentration of 781.4 and 355.4mg/L at 36h of growth, respectively. These results indicate the potential of B. subtilis EA-CB0015 to produce high concentrations of biomass and lipopeptides that can be exploited for biotechnological application. © 2014 Elsevier Ltd.eng
dc.identifierhttps://eafit.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=2024
dc.identifier.doi10.1016/j.bcab.2014.09.004spa
dc.identifier.issn18788181spa
dc.identifier.otherWOS;000332389000133
dc.identifier.otherPUBMED;24587220
dc.identifier.urihttp://hdl.handle.net/10784/26801
dc.language.isoengeng
dc.publisherElsevier Ltd
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84914809823&doi=10.1016%2fj.bcab.2014.09.004&partnerID=40&md5=ee34014b599266dd3d90fccd3bed3e13
dc.rightshttps://v2.sherpa.ac.uk/id/publication/issn/1878-8181
dc.sourceBiocatalysis and Agricultural Biotechnology
dc.subject.keywordBacillus subtiliseng
dc.subject.keywordMycosphaerella fijiensiseng
dc.titleMultiple response optimization of Bacillus subtilis EA-CB0015 culture and identification of antifungal metaboliteseng
dc.typearticleeng
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.typepublishedVersioneng
dc.type.localArtículo

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