Effect of medium components and culture conditions in Bacillus subtilis EA-CB0575 spore production

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dc.citation.journalTitleBioprocess and Biosystems Engineeringspa
dc.contributor.authorPosada-Uribe, Luisa F.
dc.contributor.authorRomero-Tabarez, Magally
dc.contributor.authorVillegas-Escobar, Valeska
dc.contributor.departmentUniversidad EAFIT. Departamento de Cienciasspa
dc.contributor.researchgroupCiencias Biológicas y Bioprocesos (CIBIOP)spa
dc.date.accessioned2021-03-23T20:08:56Z
dc.date.available2021-03-23T20:08:56Z
dc.date.issued2015-10-01
dc.description.abstractBacillus subtilis spores have important biotechnological applications; however, achieving both, high spore cell densities and sporulation efficiencies in fermentation, is poorly reported. In this study, medium components and culture conditions were optimized with different statistical methods to increase spore production of the plant growth promoting rhizobacteria B. subtilis EA-CB0575. Key medium components were determined with Plackett-Burman (PB) design, and the optimum concentration levels of two components (glucose, MgSO4·7H2O) were optimized with a full factorial and central composite design, achieving 1.37 × 10(9) CFU/mL of spore cell density and 93.5 % of sporulation efficiency in shake flask. The optimized medium was used to determine the effect of culture conditions on spore production at bioreactor level, finding that maintaining pH control did not affect significantly spore production, while the interaction of agitation and aeration rates had a significant effect on spore cell density. The overall optimization generated a 17.2-fold increase in spore cell density (8.78 × 10(9) CFU/mL) and 1.9-fold increase in sporulation efficiency (94.2 %) compared to that of PB design. These results indicate the potential of B. subtilis EA-CB0575 to produce both, high spore cell densities and sporulation efficiencies, with very low nutrient requirements and short incubation period which can represent savings of process production.eng
dc.identifierhttps://eafit.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=1988
dc.identifier.doi10.1007/s00449-015-1428-1spa
dc.identifier.issn16157591spa
dc.identifier.issn16157605spa
dc.identifier.otherWOS;000382346300024
dc.identifier.otherPUBMED;27263830
dc.identifier.otherSCOPUS;2-s2.0-84979498465
dc.identifier.urihttp://hdl.handle.net/10784/26799
dc.language.isoengeng
dc.publisherSpringer Berlin Heidelberg
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85027957022&doi=10.1007%2fs00449-015-1428-1&partnerID=40&md5=97385d3d4235c5a8db89db68d686ec09
dc.rightshttps://v2.sherpa.ac.uk/id/publication/issn/1615-7591
dc.sourceBioprocess and Biosystems Engineering
dc.subject.keywordculture mediumeng
dc.subject.keywordBacillus subtiliseng
dc.subject.keywordbacterial sporeeng
dc.subject.keywordbatch cell cultureeng
dc.subject.keywordbioreactoreng
dc.subject.keywordcell culture techniqueeng
dc.subject.keywordcell proliferationeng
dc.subject.keywordclassificationeng
dc.subject.keywordculture mediumeng
dc.subject.keywordcytologyeng
dc.subject.keywordgrowtheng
dc.subject.keyworddevelopment and agingeng
dc.subject.keywordmetabolismeng
dc.subject.keywordmicrobiologyeng
dc.subject.keywordphysiologyeng
dc.subject.keywordprocedureseng
dc.subject.keywordspecies differenceeng
dc.subject.keywordBacillus subtiliseng
dc.subject.keywordBatch Cell Culture Techniqueseng
dc.subject.keywordBioreactorseng
dc.subject.keywordCell Culture Techniqueseng
dc.subject.keywordCell Proliferationeng
dc.subject.keywordCulture Mediaeng
dc.subject.keywordSpecies Specificityeng
dc.subject.keywordSporeseng
dc.subject.keywordBacterialeng
dc.titleEffect of medium components and culture conditions in Bacillus subtilis EA-CB0575 spore productioneng
dc.typearticleeng
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.typepublishedVersioneng
dc.type.localArtículo

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