Ceratocystis cacaofunesta genome analysis reveals a large expansion of extracellular phosphatidylinositol-specific phospholipase-C genes (PI-PLC)

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dc.citation.journalTitleBmc Genomicsspa
dc.contributor.authorMolano, E.P.L.
dc.contributor.authorCabrera, O.G.
dc.contributor.authorJose, J.
dc.contributor.authordo Nascimento, L.C.
dc.contributor.authorCarazzolle, M.F.
dc.contributor.authorTeixeira, P.J.P.L.
dc.contributor.authorAlvarez, J.C.
dc.contributor.authorTiburcio, R.A.
dc.contributor.authorTokimatu Filho, P.M.
dc.contributor.authorde Lima, G.M.A.
dc.contributor.authorGuido, R.V.C.
dc.contributor.authorCorrêa, T.L.R.
dc.contributor.authorLeme, A.F.P.
dc.contributor.authorMieczkowski, P.
dc.contributor.authorPereira, G.A.G.
dc.contributor.departmentUniversidad EAFIT. Departamento de Cienciasspa
dc.contributor.researchgroupCiencias Biológicas y Bioprocesos (CIBIOP)spa
dc.date.accessioned2021-03-23T20:09:00Z
dc.date.available2021-03-23T20:09:00Z
dc.date.issued2018-01-17
dc.description.abstractBackground: The Ceratocystis genus harbors a large number of phytopathogenic fungi that cause xylem parenchyma degradation and vascular destruction on a broad range of economically important plants. Ceratocystis cacaofunesta is a necrotrophic fungus responsible for lethal wilt disease in cacao. The aim of this work is to analyze the genome of C. cacaofunesta through a comparative approach with genomes of other Sordariomycetes in order to better understand the molecular basis of pathogenicity in the Ceratocystis genus. Results: We present an analysis of the C. cacaofunesta genome focusing on secreted proteins that might constitute pathogenicity factors. Comparative genome analyses among five Ceratocystidaceae species and 23 other Sordariomycetes fungi showed a strong reduction in gene content of the Ceratocystis genus. However, some gene families displayed a remarkable expansion, in particular, the Phosphatidylinositol specific phospholipases-C (PI-PLC) family. Also, evolutionary rate calculations suggest that the evolution process of this family was guided by positive selection. Interestingly, among the 82 PI-PLCs genes identified in the C. cacaofunesta genome, 70 genes encoding extracellular PI-PLCs are grouped in eight small scaffolds surrounded by transposon fragments and scars that could be involved in the rapid evolution of the PI-PLC family. Experimental secretome using LC-MS/MS validated 24% (86 proteins) of the total predicted secretome (342 proteins), including four PI-PLCs and other important pathogenicity factors. Conclusion: Analysis of the Ceratocystis cacaofunesta genome provides evidence that PI-PLCs may play a role in pathogenicity. Subsequent functional studies will be aimed at evaluating this hypothesis. The observed genetic arsenals, together with the analysis of the PI-PLC family shown in this work, reveal significant differences in the Ceratocystis genome compared to the classical vascular fungi, Verticillium and Fusarium. Altogether, our analyses provide new insights into the evolution and the molecular basis of plant pathogenicity. © 2018 The Author(s).eng
dc.identifierhttps://eafit.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=7940
dc.identifier.doi10.1186/s12864-018-4440-4spa
dc.identifier.issn14712164spa
dc.identifier.otherSCOPUS;2-s2.0-85062326067
dc.identifier.urihttp://hdl.handle.net/10784/26829
dc.language.isoengeng
dc.publisherBioMed Central Ltd.
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85040746089&doi=10.1186%2fs12864-018-4440-4&partnerID=40&md5=6058b6aedd5783c9f58d4dd67875f57a
dc.rightshttps://v2.sherpa.ac.uk/id/publication/issn/1471-2164
dc.sourceBmc Genomics
dc.subject.keywordphosphatidylinositol 4,5 bisphosphate phosphodiesteraseeng
dc.subject.keywordvirulence factoreng
dc.subject.keywordfungal proteineng
dc.subject.keywordphosphatidylinositoleng
dc.subject.keywordphosphatidylinositol 4,5 bisphosphate phosphodiesteraseeng
dc.subject.keywordArticleeng
dc.subject.keywordCeratocystiseng
dc.subject.keywordCeratocystis cacaofunestaeng
dc.subject.keywordevolutionary rateeng
dc.subject.keywordfungal geneeng
dc.subject.keywordfungal genomeeng
dc.subject.keywordfungal virulenceeng
dc.subject.keywordFusariumeng
dc.subject.keywordliquid chromatography-mass spectrometryeng
dc.subject.keywordnatural selectioneng
dc.subject.keywordnonhumaneng
dc.subject.keywordplant fungus interactioneng
dc.subject.keywordpositive selectioneng
dc.subject.keywordprotein hydrolysiseng
dc.subject.keywordprotein secretioneng
dc.subject.keywordSordariomyceteseng
dc.subject.keywordtransposoneng
dc.subject.keywordVerticilliumeng
dc.subject.keywordAscomyceteseng
dc.subject.keywordcacaoeng
dc.subject.keywordchemistryeng
dc.subject.keywordgene expression regulationeng
dc.subject.keywordgeneticseng
dc.subject.keywordgenomicseng
dc.subject.keywordmetabolismeng
dc.subject.keywordmicrobiologyeng
dc.subject.keywordmolecular evolutioneng
dc.subject.keywordphylogenyeng
dc.subject.keywordprocedureseng
dc.subject.keywordprotein conformationeng
dc.subject.keywordAscomycotaeng
dc.subject.keywordCacaoeng
dc.subject.keywordEvolutioneng
dc.subject.keywordMoleculareng
dc.subject.keywordFungal Proteinseng
dc.subject.keywordGene Expression Regulationeng
dc.subject.keywordPlanteng
dc.subject.keywordGenomeeng
dc.subject.keywordFungaleng
dc.subject.keywordGenomicseng
dc.subject.keywordPhosphatidylinositolseng
dc.subject.keywordPhosphoinositide Phospholipase Ceng
dc.subject.keywordPhylogenyeng
dc.subject.keywordProtein Conformationeng
dc.titleCeratocystis cacaofunesta genome analysis reveals a large expansion of extracellular phosphatidylinositol-specific phospholipase-C genes (PI-PLC)eng
dc.typearticleeng
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.typepublishedVersioneng
dc.type.localArtículo

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