Scale-up from shake flasks to pilot-scale production of the plant growth-promoting bacterium Azospirillum brasilense for preparing a liquid inoculant formulation

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dc.citation.journalTitleApplied Microbiology and Biotechnologyspa
dc.contributor.authorVillegas, Jesús
dc.contributor.authorTrujillo Roldán, Mauricio Alberto
dc.contributor.authorBlancas Cabrera, Abel
dc.contributor.authorGamboa Suasnavart, Ramsés Adolfo
dc.contributor.authorGarcía Cabrera, Ramsés I.
dc.contributor.authorAcevedo Sánchez, Eduardo V.
dc.contributor.authorValdez Cruz, Norma Adriana
dc.contributor.authorGonzález Monterrubio, César Fernando
dc.contributor.authorMartínez Salinas, Carlos
dc.contributor.authorMarín Palacio, Luz Deisy
dc.contributor.departmentUnidad de Bioprocesos, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México
dc.contributor.departmentBiofábrica Siglo XXI S.A. de C.V., Carretera México-Oaxaca Km 106, Col
dc.contributor.departmentUniversidad EAFIT. Departamento de Cienciasspa
dc.contributor.eafitauthorMauricio A. Trujillo Roldán (maurotru@biomedicas.unam.mx)spa
dc.contributor.researchgroupCiencias Biológicas y Bioprocesos (CIBIOP)spa
dc.date.accessioned2015-08-13T14:20:10Z
dc.date.available2015-08-13T14:20:10Z
dc.date.issued2013
dc.description.abstractAzospirillum brasilense has industrial significance as a growth promoter in plants of commercial interest -- However, there is no report in the literature disclosing a liquid product produced in pilot-scale bioreactors and is able to be stored at room temperature for more than 2 years -- The aim of this work was to scale up a process from a shake flask to a 10- L lab-scale and 1,000-L pilot-scale bioreactor for the production of plant growth-promoting bacterium A -- brasilense for a liquid inoculant formulation -- Furthermore, this work aimed to determine the shelf life of the liquid formulation stored at room temperature and to increase maize crops yield in greenhouses -- Under a constant oxygen mass transfer coefficient (KLa), a fermentation process was successfully scaled up from shake flasks to 10- and 1,000-L bioreactors -- A concentration ranging from 3.5 to 7.5×108 CFU/mL was obtained in shake flasks and bioreactors, and after 2 years stored at room temperature, the liquid formulation showed one order of magnitude decrease -- Applications of the cultured bacteria in maize yields resulted in increases of up to 95 % in corncobs and 70 % in aboveground biomasseng
dc.identifier.doi10.1007/s00253-013-5199-9spa
dc.identifier.issn0175-7598spa
dc.identifier.issn1432-0614spa
dc.identifier.otherWOS;000233344100002
dc.identifier.otherPUBMED;16226709
dc.identifier.otherSCOPUS;2-s2.0-27644577029
dc.identifier.urihttp://hdl.handle.net/10784/7256
dc.language.isoengeng
dc.publisherSpringer Verlagspa
dc.relation.ispartofApplied Microbiology and Biotechnologyspa
dc.relation.urihttp://www.ncbi.nlm.nih.gov/pubmed/24061414
dc.rightsSpringer-Verlag Berlin Heidelberg 2013spa
dc.rights.accessrightsinfo:eu-repo/semantics/restrictedAccessspa
dc.rights.localAcceso restringidospa
dc.subject.keywordAzospirillumeng
dc.subject.keywordFermentationeng
dc.subject.keywordCulture techniques(Biology)eng
dc.subject.keywordNitrogen - fixationeng
dc.subject.keywordBioreactorseng
dc.subject.keywordBacterial growtheng
dc.subject.keywordBiomasa aéreaspa
dc.subject.keywordConcentración bacterianaspa
dc.subject.keywordRizobacteriasspa
dc.subject.keywordBiofertilizantespa
dc.subject.lembAZOSPIRILLUMspa
dc.subject.lembINOCULACIÓNspa
dc.subject.lembFERMENTACIÓNspa
dc.subject.lembTÉCNICAS DE CULTIVOspa
dc.subject.lembFIJACIÓN DEL NITRÓGENOspa
dc.subject.lembBIOREACTORESspa
dc.subject.lembCRECIMIENTO (BACTERIAS)spa
dc.titleScale-up from shake flasks to pilot-scale production of the plant growth-promoting bacterium Azospirillum brasilense for preparing a liquid inoculant formulationeng
dc.typearticleeng
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.typepublishedVersioneng
dc.type.localArtículo

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