Paracoccidioides spp. catalases and their role in antioxidant defense against host defense responses

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dc.citation.journalTitleFungal Genetics and Biologyspa
dc.contributor.authorTamayo, D.
dc.contributor.authorMuñoz, J.F.
dc.contributor.authorAlmeida, A.J.
dc.contributor.authorPuerta, J.D.
dc.contributor.authorRestrepo, Á.
dc.contributor.authorCuomo, C.A.
dc.contributor.authorMcEwen, J.G.
dc.contributor.authorHernández, O.
dc.contributor.departmentUniversidad EAFIT. Departamento de Cienciasspa
dc.contributor.researchgroupCiencias Biológicas y Bioprocesos (CIBIOP)spa
dc.date.accessioned2021-03-23T20:08:59Z
dc.date.available2021-03-23T20:08:59Z
dc.date.issued2017-03-01
dc.description.abstractDimorphic human pathogenic fungi interact with host effector cells resisting their microbicidal mechanisms. Yeast cells are able of surviving within the tough environment of the phagolysosome by expressing an antioxidant defense system that provides protection against host-derived reactive oxygen species (ROS). This includes the production of catalases (CATs). Here we identified and analyzed the role of CAT isoforms in Paracoccidioides, the etiological agent of paracoccidioidomycosis. Firstly, we found that one of these isoforms was absent in the closely related dimorphic pathogen Coccidioides and dermatophytes, but all of them were conserved in Paracoccidioides, Histoplasma and Blastomyces species. We probed the contribution of CATs in Paracoccidioides by determining the gene expression levels of each isoform through quantitative RT-qPCR, in both the yeast and mycelia phases, and during the morphological switch (transition and germination), as well as in response to oxidative agents and during interaction with neutrophils. PbCATP was preferentially expressed in the pathogenic yeast phase, and was associated to the response against exogenous H2O2. Therefore, we created and analyzed the virulence defects of a knockdown strain for this isoform, and found that CATP protects yeast cells from H2O2 generated in vitro and is relevant during lung infection. On the other hand, CATA and CATB seem to contribute to ROS homeostasis in Paracoccidioides cells, during endogenous oxidative stress. CAT isoforms in Paracoccidioides might be coordinately regulated during development and dimorphism, and differentially expressed in response to different stresses to control ROS homeostasis during the infectious process, contributing to the virulence of Paracoccidioides. © 2017 Elsevier Inc.eng
dc.identifierhttps://eafit.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=6257
dc.identifier.doi10.1016/j.fgb.2017.01.005spa
dc.identifier.issn10871845spa
dc.identifier.issn10960937spa
dc.identifier.otherWOS;000411473000024
dc.identifier.otherSCOPUS;2-s2.0-85028744140
dc.identifier.urihttp://hdl.handle.net/10784/26822
dc.language.isoengeng
dc.publisherElsevier
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85010059648&doi=10.1016%2fj.fgb.2017.01.005&partnerID=40&md5=48f3204771d9a9ac50a2264e88978f43
dc.rightshttps://v2.sherpa.ac.uk/id/publication/issn/1087-1845
dc.sourceFungal Genetics and Biology
dc.subject.keywordantioxidanteng
dc.subject.keywordcatalaseeng
dc.subject.keywordhydrogen peroxideeng
dc.subject.keywordisoenzymeeng
dc.subject.keywordreactive oxygen metaboliteeng
dc.subject.keywordantioxidanteng
dc.subject.keywordcatalaseeng
dc.subject.keywordanimal experimenteng
dc.subject.keywordanimal modeleng
dc.subject.keywordanimal tissueeng
dc.subject.keywordArticleeng
dc.subject.keywordCATA geneeng
dc.subject.keywordCATB geneeng
dc.subject.keywordCATP geneeng
dc.subject.keywordcell interactioneng
dc.subject.keywordcell protectioneng
dc.subject.keywordcontrolled studyeng
dc.subject.keywordenzyme activityeng
dc.subject.keywordfungal celleng
dc.subject.keywordfungal geneeng
dc.subject.keywordfungal straineng
dc.subject.keywordfungal virulenceeng
dc.subject.keywordfungus growtheng
dc.subject.keywordgene expressioneng
dc.subject.keywordgerminationeng
dc.subject.keywordhomeostasiseng
dc.subject.keywordhost pathogen interactioneng
dc.subject.keywordin vitro studyeng
dc.subject.keywordlung infectioneng
dc.subject.keywordmaleeng
dc.subject.keywordmouseeng
dc.subject.keywordmyceliumeng
dc.subject.keywordmycosiseng
dc.subject.keywordneutrophileng
dc.subject.keywordnonhumaneng
dc.subject.keywordParacoccidioideseng
dc.subject.keywordsex differenceeng
dc.subject.keywordchemistryeng
dc.subject.keywordenzymologyeng
dc.subject.keywordgene expression regulationeng
dc.subject.keywordgeneticseng
dc.subject.keywordHistoplasmaeng
dc.subject.keywordhumaneng
dc.subject.keywordmetabolismeng
dc.subject.keywordmicrobiologyeng
dc.subject.keywordoxidative stresseng
dc.subject.keywordParacoccidioideseng
dc.subject.keywordSouth American blastomycosiseng
dc.subject.keywordAntioxidantseng
dc.subject.keywordCatalaseeng
dc.subject.keywordGene Expression Regulationeng
dc.subject.keywordFungaleng
dc.subject.keywordHistoplasmaeng
dc.subject.keywordHumanseng
dc.subject.keywordHydrogen Peroxideeng
dc.subject.keywordMyceliumeng
dc.subject.keywordOxidative Stresseng
dc.subject.keywordParacoccidioideseng
dc.subject.keywordParacoccidioidomycosiseng
dc.subject.keywordReactive Oxygen Specieseng
dc.titleParacoccidioides spp. catalases and their role in antioxidant defense against host defense responseseng
dc.typearticleeng
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.typepublishedVersioneng
dc.type.localArtículo

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