Enhanced molecular visualization of root colonization and growth promotion by Bacillus subtilis EA-CB0575 in different growth systems

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dc.citation.journalTitleMICROBIOLOGICAL RESEARCHspa
dc.contributor.authorPosada, L.F.
dc.contributor.authorÁlvarez, J.C.
dc.contributor.authorRomero-Tabarez, M.
dc.contributor.authorde-Bashan, L.
dc.contributor.authorVillegas-Escobar, V.
dc.contributor.departmentUniversidad EAFIT. Departamento de Cienciasspa
dc.contributor.researchgroupCiencias Biológicas y Bioprocesos (CIBIOP)spa
dc.date.accessioned2021-03-23T20:09:00Z
dc.date.available2021-03-23T20:09:00Z
dc.date.issued2018-01-01
dc.description.abstractBacillus subtilis EA-CB0575 is a plant growth-promoting bacterium (PGPB) associated with banana and tomato crops. Root colonization is an important trait for PGPB microorganisms and potentiates the bacterial effect related to the mechanisms of plant growth promotion. Therefore, detection of bacterial colonization of roots in different culture systems is important in the study of plant–microorganism interactions. In this study, fluorescent in situ hybridization (FISH) and catalyzed reporter deposition–FISH (CARD–FISH) were evaluated to determine the colonization ability of B. subtilis EA-CB0575 on banana and tomato roots planted on solid and liquid Murashige and Skoog medium (MS(S) and MS(L), respectively) and in soil for tomato plants. Results showed B. subtilis colonization 0–30 days post inoculation for banana and tomato plants in different culture systems with differential distribution of bacterial cells along tomato and banana roots. FISH and CARD–FISH methodologies were both successful in detecting B. subtilis colonies, but CARD–FISH proved to be superior due to its enhanced fluorescence signal. The presence of bacteria correlated with the promotion of plant growth in both plant species, providing clues to relate rhizospheric colonization with improvement in plant growth. FISH and CARD–FISH analysis results suggested the presence of native microbiota on the roots of in vitro banana plants, but not on those of tomato plants. © 2018 Elsevier GmbHeng
dc.identifierhttps://eafit.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=8198
dc.identifier.doi10.1016/j.micres.2018.08.017spa
dc.identifier.issn09445013spa
dc.identifier.issn16180623spa
dc.identifier.otherWOS;000505657700017
dc.identifier.otherSCOPUS;2-s2.0-85076745823
dc.identifier.urihttp://hdl.handle.net/10784/26831
dc.language.isoengeng
dc.publisherElsevier GmbH
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85053471614&doi=10.1016%2fj.micres.2018.08.017&partnerID=40&md5=1188ac8fc133d0eed23a60c1c807ddd0
dc.rightshttps://v2.sherpa.ac.uk/id/publication/issn/0944-5013
dc.sourceMICROBIOLOGICAL RESEARCH
dc.subject.keywordActivation analysiseng
dc.subject.keywordBacteriologyeng
dc.subject.keywordCatalysiseng
dc.subject.keywordDepositioneng
dc.subject.keywordFisheng
dc.subject.keywordFluorescenceeng
dc.subject.keywordFluorescence microscopyeng
dc.subject.keywordFruitseng
dc.subject.keywordPlants (botany)eng
dc.subject.keywordBacillus speng
dc.subject.keywordFluorescence in situ hybridizationeng
dc.subject.keywordFluorescent in situ hybridizationeng
dc.subject.keywordMolecular visualizationeng
dc.subject.keywordMurashige and Skoog mediumseng
dc.subject.keywordPlant growth promoting bacteriumeng
dc.subject.keywordPlant growth promotioneng
dc.subject.keywordRoot colonizationeng
dc.subject.keywordBacteriaeng
dc.subject.keywordbacteriumeng
dc.subject.keywordcelleng
dc.subject.keywordcolonyeng
dc.subject.keywordgenetic analysiseng
dc.subject.keywordgrowtheng
dc.subject.keywordherbeng
dc.subject.keywordmicrobial activityeng
dc.subject.keywordmolecular analysiseng
dc.subject.keywordplanteng
dc.subject.keywordrhizosphereeng
dc.subject.keywordroot colonizationeng
dc.subject.keywordroot systemeng
dc.subject.keywordBacillus sp.eng
dc.subject.keywordBacillus subtiliseng
dc.subject.keywordBacteria (microorganisms)eng
dc.subject.keywordLycopersicon esculentumeng
dc.subject.keywordagricultural inoculationeng
dc.subject.keywordBacillus subtiliseng
dc.subject.keywordfluorescence in situ hybridizationeng
dc.subject.keywordgrowtheng
dc.subject.keyworddevelopment and agingeng
dc.subject.keywordmicrobiologyeng
dc.subject.keywordmicrofloraeng
dc.subject.keywordMusaeng
dc.subject.keywordphysiologyeng
dc.subject.keywordplant developmenteng
dc.subject.keywordplant rooteng
dc.subject.keywordplant seedeng
dc.subject.keywordrhizosphereeng
dc.subject.keywordscanning electron microscopyeng
dc.subject.keywordsoileng
dc.subject.keywordtomatoeng
dc.subject.keywordAgricultural Inoculantseng
dc.subject.keywordBacillus subtiliseng
dc.subject.keywordIn Situ Hybridizationeng
dc.subject.keywordFluorescenceeng
dc.subject.keywordLycopersicon esculentumeng
dc.subject.keywordMicrobiotaeng
dc.subject.keywordMicroscopyeng
dc.subject.keywordElectroneng
dc.subject.keywordScanningeng
dc.subject.keywordMusaeng
dc.subject.keywordPeng
dc.titleEnhanced molecular visualization of root colonization and growth promotion by Bacillus subtilis EA-CB0575 in different growth systemseng
dc.typearticleeng
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.typepublishedVersioneng
dc.type.localArtículo

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