Scale-up from shake flasks to pilot-scale production of the plant growth-promoting bacterium Azospirillum brasilense for preparing a liquid inoculant formulation

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dc.citation.journalTitleAPPLIED MICROBIOLOGY AND BIOTECHNOLOGYspa
dc.contributor.authorTrujillo-Roldan, Mauricio A.
dc.contributor.authorValdez-Cruz, Norma A.
dc.contributor.authorGonzalez-Monterrubio, Cesar F.
dc.contributor.authorAcevedo-Sanchez, Eduardo V.
dc.contributor.authorMartinez-Salinas, Carlos
dc.contributor.authorGarcia-Cabrera, Ramses I.
dc.contributor.authorGamboa-Suasnavart, Ramses A.
dc.contributor.authorMarin-Palacio, Luz D.
dc.contributor.authorVillegas, Jesus
dc.contributor.authorBlancas-Cabrera, Abel
dc.contributor.departmentUniversidad EAFIT. Departamento de Cienciasspa
dc.contributor.researchgroupCiencias Biológicas y Bioprocesos (CIBIOP)spa
dc.date.accessioned2021-03-23T20:08:54Z
dc.date.available2021-03-23T20:08:54Z
dc.date.issued2013-11-01
dc.description.abstractAzospirillum brasilense has industrial significance as a growth promoter in plants of commercial interest. However, there is no report in the literature disclosing a liquid product produced in pilot-scale bioreactors and is able to be stored at room temperature for more than 2 years. The aim of this work was to scale up a process from a shake flask to a 10-L lab-scale and 1,000-L pilot-scale bioreactor for the production of plant growth-promoting bacterium A. brasilense for a liquid inoculant formulation. Furthermore, this work aimed to determine the shelf life of the liquid formulation stored at room temperature and to increase maize crops yield in greenhouses. Under a constant oxygen mass transfer coefficient (K L a), a fermentation process was successfully scaled up from shake flasks to 10- and 1,000-L bioreactors. A concentration ranging from 3.5 to 7.5 × 108 CFU/mL was obtained in shake flasks and bioreactors, and after 2 years stored at room temperature, the liquid formulation showed one order of magnitude decrease. Applications of the cultured bacteria in maize yields resulted in increases of up to 95 % in corncobs and 70 % in aboveground biomass. © 2013 Springer-Verlag Berlin Heidelberg.eng
dc.identifierhttps://eafit.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=1291
dc.identifier.doi10.1007/s00253-013-5199-9spa
dc.identifier.issn01757598spa
dc.identifier.issn14320614spa
dc.identifier.otherWOS;000351609200004
dc.identifier.otherSCOPUS;2-s2.0-84925331497
dc.identifier.urihttp://hdl.handle.net/10784/26789
dc.language.isoengeng
dc.publisherSPRINGER
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84888435786&doi=10.1007%2fs00253-013-5199-9&partnerID=40&md5=b83617d3b2eb42cb5ccd64bfbf7a0acd
dc.rightshttps://v2.sherpa.ac.uk/id/publication/issn/0175-7598
dc.sourceAPPLIED MICROBIOLOGY AND BIOTECHNOLOGY
dc.subject.keywordAzospirillum brasilenseeng
dc.subject.keywordCorneng
dc.subject.keywordOxygen mass transfereng
dc.subject.keywordRhizosphereeng
dc.subject.keywordScale-upeng
dc.subject.keywordBacteriaeng
dc.subject.keywordBioconversioneng
dc.subject.keywordBioreactorseng
dc.subject.keywordBottleseng
dc.subject.keywordFermentationeng
dc.subject.keywordMass transfereng
dc.subject.keywordOxygeneng
dc.subject.keywordPlant life extensioneng
dc.subject.keywordSoilseng
dc.subject.keywordLiquidseng
dc.subject.keywordoxygeneng
dc.subject.keywordaboveground biomasseng
dc.subject.keywordbacteriumeng
dc.subject.keywordbioreactoreng
dc.subject.keywordcrop improvementeng
dc.subject.keywordcrop yieldeng
dc.subject.keywordgrowth regulatoreng
dc.subject.keywordinoculationeng
dc.subject.keywordmaizeeng
dc.subject.keywordmass transfereng
dc.subject.keywordmicrobial activityeng
dc.subject.keywordoxygeneng
dc.subject.keywordrhizosphereeng
dc.subject.keywordarticleeng
dc.subject.keywordAzospirillum brasilenseeng
dc.subject.keywordbiomasseng
dc.subject.keywordbioreactoreng
dc.subject.keywordfermentationeng
dc.subject.keywordgreenhouseeng
dc.subject.keywordmaizeeng
dc.subject.keywordoxygen transporteng
dc.subject.keywordplant growtheng
dc.subject.keywordplant-growth promoting bacteriumeng
dc.subject.keywordroom temperatureeng
dc.subject.keywordscale upeng
dc.subject.keywordshelf lifeeng
dc.subject.keywordsoil inoculationeng
dc.subject.keywordAzospirillum brasilenseeng
dc.subject.keywordBacteria (microorganisms)eng
dc.subject.keywordZea mayseng
dc.subject.keywordAzospirillum brasilenseeng
dc.subject.keywordBacterial Loadeng
dc.subject.keywordColony Counteng
dc.subject.keywordMicrobialeng
dc.subject.keywordIndustrial Microbiologyeng
dc.subject.keywordPreservationeng
dc.subject.keywordBiologicaleng
dc.subject.keywordTime Factorseng
dc.subject.keywordZea mayseng
dc.titleScale-up from shake flasks to pilot-scale production of the plant growth-promoting bacterium Azospirillum brasilense for preparing a liquid inoculant formulationeng
dc.typearticleeng
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.typepublishedVersioneng
dc.type.localArtículo

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