Scale-up from shake flasks to pilot-scale production of the plant growth-promoting bacterium Azospirillum brasilense for preparing a liquid inoculant formulation

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dc.citation.journalTitleAPPLIED MICROBIOLOGY AND BIOTECHNOLOGY
dc.contributor.authorTrujillo-Roldan, Mauricio A.
dc.contributor.authorValdez-Cruz, Norma A.
dc.contributor.authorGonzalez-Monterrubio, Cesar F.
dc.contributor.authorAcevedo-Sanchez, Eduardo V.
dc.contributor.authorMartinez-Salinas, Carlos
dc.contributor.authorGarcia-Cabrera, Ramses I.
dc.contributor.authorGamboa-Suasnavart, Ramses A.
dc.contributor.authorMarin-Palacio, Luz D.
dc.contributor.authorVillegas, Jesus
dc.contributor.authorBlancas-Cabrera, Abel
dc.contributor.departmentUniversidad EAFIT. Departamento de Ingeniería de Procesosspa
dc.contributor.researchgroupProcesos Ambientales (GIPAB)spa
dc.creatorTrujillo-Roldan, Mauricio A.
dc.creatorValdez-Cruz, Norma A.
dc.creatorGonzalez-Monterrubio, Cesar F.
dc.creatorAcevedo-Sanchez, Eduardo V.
dc.creatorMartinez-Salinas, Carlos
dc.creatorGarcia-Cabrera, Ramses I.
dc.creatorGamboa-Suasnavart, Ramses A.
dc.creatorMarin-Palacio, Luz D.
dc.creatorVillegas, Jesus
dc.creatorBlancas-Cabrera, Abel
dc.date.accessioned2021-04-16T20:27:35Z
dc.date.available2021-04-16T20:27:35Z
dc.date.issued2013-11-01
dc.description.abstractAzospirillum brasilense has industrial significance as a growth promoter in plants of commercial interest. However, there is no report in the literature disclosing a liquid product produced in pilot-scale bioreactors and is able to be stored at room temperature for more than 2 years. The aim of this work was to scale up a process from a shake flask to a 10-L lab-scale and 1,000-L pilot-scale bioreactor for the production of plant growth-promoting bacterium A. brasilense for a liquid inoculant formulation. Furthermore, this work aimed to determine the shelf life of the liquid formulation stored at room temperature and to increase maize crops yield in greenhouses. Under a constant oxygen mass transfer coefficient (K L a), a fermentation process was successfully scaled up from shake flasks to 10- and 1,000-L bioreactors. A concentration ranging from 3.5 to 7.5 × 108 CFU/mL was obtained in shake flasks and bioreactors, and after 2 years stored at room temperature, the liquid formulation showed one order of magnitude decrease. Applications of the cultured bacteria in maize yields resulted in increases of up to 95 % in corncobs and 70 % in aboveground biomass. © 2013 Springer-Verlag Berlin Heidelberg.eng
dc.identifierhttps://eafit.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=1291
dc.identifier.doi10.1007/s00253-013-5199-9
dc.identifier.issn01757598
dc.identifier.issn14320614
dc.identifier.otherWOS;000326394000008
dc.identifier.otherPUBMED;24061414
dc.identifier.otherSCOPUS;2-s2.0-84888435786
dc.identifier.urihttp://hdl.handle.net/10784/29363
dc.language.isoeng
dc.publisherSPRINGER
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84888435786&doi=10.1007%2fs00253-013-5199-9&partnerID=40&md5=b83617d3b2eb42cb5ccd64bfbf7a0acd
dc.rightshttps://v2.sherpa.ac.uk/id/publication/issn/0175-7598
dc.sourceAPPLIED MICROBIOLOGY AND BIOTECHNOLOGY
dc.subjectAzospirillum brasilenseeng
dc.subjectCorneng
dc.subjectOxygen mass transfereng
dc.subjectRhizosphereeng
dc.subjectScale-upeng
dc.subjectBacteriaeng
dc.subjectBioconversioneng
dc.subjectBioreactorseng
dc.subjectBottleseng
dc.subjectFermentationeng
dc.subjectMass transfereng
dc.subjectOxygeneng
dc.subjectPlant life extensioneng
dc.subjectSoilseng
dc.subjectLiquidseng
dc.subjectoxygeneng
dc.subjectaboveground biomasseng
dc.subjectbacteriumeng
dc.subjectbioreactoreng
dc.subjectcrop improvementeng
dc.subjectcrop yieldeng
dc.subjectgrowth regulatoreng
dc.subjectinoculationeng
dc.subjectmaizeeng
dc.subjectmass transfereng
dc.subjectmicrobial activityeng
dc.subjectoxygeneng
dc.subjectrhizosphereeng
dc.subjectarticleeng
dc.subjectAzospirillum brasilenseeng
dc.subjectbiomasseng
dc.subjectbioreactoreng
dc.subjectfermentationeng
dc.subjectgreenhouseeng
dc.subjectmaizeeng
dc.subjectoxygen transporteng
dc.subjectplant growtheng
dc.subjectplant-growth promoting bacteriumeng
dc.subjectroom temperatureeng
dc.subjectscale upeng
dc.subjectshelf lifeeng
dc.subjectsoil inoculationeng
dc.subjectAzospirillum brasilenseeng
dc.subjectBacteria (microorganisms)eng
dc.subjectZea mayseng
dc.subjectAzospirillum brasilenseeng
dc.subjectBacterial Loadeng
dc.subjectColony Counteng
dc.subjectMicrobialeng
dc.subjectIndustrial Microbiologyeng
dc.subjectPreservationeng
dc.subjectBiologicaleng
dc.subjectTime Factorseng
dc.subjectZea mayseng
dc.titleScale-up from shake flasks to pilot-scale production of the plant growth-promoting bacterium Azospirillum brasilense for preparing a liquid inoculant formulationeng
dc.typeinfo:eu-repo/semantics/articleeng
dc.typearticleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.typepublishedVersioneng
dc.type.localArtículospa

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