Scale-up from shake flasks to pilot-scale production of the plant growth-promoting bacterium Azospirillum brasilense for preparing a liquid inoculant formulation
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2013-11-01
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SPRINGER
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Azospirillum brasilense has industrial significance as a growth promoter in plants of commercial interest. However, there is no report in the literature disclosing a liquid product produced in pilot-scale bioreactors and is able to be stored at room temperature for more than 2 years. The aim of this work was to scale up a process from a shake flask to a 10-L lab-scale and 1,000-L pilot-scale bioreactor for the production of plant growth-promoting bacterium A. brasilense for a liquid inoculant formulation. Furthermore, this work aimed to determine the shelf life of the liquid formulation stored at room temperature and to increase maize crops yield in greenhouses. Under a constant oxygen mass transfer coefficient (K L a), a fermentation process was successfully scaled up from shake flasks to 10- and 1,000-L bioreactors. A concentration ranging from 3.5 to 7.5 × 108 CFU/mL was obtained in shake flasks and bioreactors, and after 2 years stored at room temperature, the liquid formulation showed one order of magnitude decrease. Applications of the cultured bacteria in maize yields resulted in increases of up to 95 % in corncobs and 70 % in aboveground biomass. © 2013 Springer-Verlag Berlin Heidelberg.
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Azospirillum brasilense, Corn, Oxygen mass transfer, Rhizosphere, Scale-up, Bacteria, Bioconversion, Bioreactors, Bottles, Fermentation, Mass transfer, Oxygen, Plant life extension, Soils, Liquids, oxygen, aboveground biomass, bacterium, bioreactor, crop improvement, crop yield, growth regulator, inoculation, maize, mass transfer, microbial activity, oxygen, rhizosphere, article, Azospirillum brasilense, biomass, bioreactor, fermentation, greenhouse, maize, oxygen transport, plant growth, plant-growth promoting bacterium, room temperature, scale up, shelf life, soil inoculation, Azospirillum brasilense, Bacteria (microorganisms), Zea mays, Azospirillum brasilense, Bacterial Load, Colony Count, Microbial, Industrial Microbiology, Preservation, Biological, Time Factors, Zea mays