Scale-up from shake flasks to pilot-scale production of the plant growth-promoting bacterium Azospirillum brasilense for preparing a liquid inoculant formulation
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Fecha
2013-11-01
Autores
Trujillo-Roldan, Mauricio A.
Valdez-Cruz, Norma A.
Gonzalez-Monterrubio, Cesar F.
Acevedo-Sanchez, Eduardo V.
Martinez-Salinas, Carlos
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SPRINGER
Resumen
Azospirillum brasilense has industrial significance as a growth promoter in plants of commercial interest. However, there is no report in the literature disclosing a liquid product produced in pilot-scale bioreactors and is able to be stored at room temperature for more than 2 years. The aim of this work was to scale up a process from a shake flask to a 10-L lab-scale and 1,000-L pilot-scale bioreactor for the production of plant growth-promoting bacterium A. brasilense for a liquid inoculant formulation. Furthermore, this work aimed to determine the shelf life of the liquid formulation stored at room temperature and to increase maize crops yield in greenhouses. Under a constant oxygen mass transfer coefficient (K L a), a fermentation process was successfully scaled up from shake flasks to 10- and 1,000-L bioreactors. A concentration ranging from 3.5 to 7.5 × 108 CFU/mL was obtained in shake flasks and bioreactors, and after 2 years stored at room temperature, the liquid formulation showed one order of magnitude decrease. Applications of the cultured bacteria in maize yields resulted in increases of up to 95 % in corncobs and 70 % in aboveground biomass. © 2013 Springer-Verlag Berlin Heidelberg.
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Azospirillum brasilense , Corn , Oxygen mass transfer , Rhizosphere , Scale-up , Bacteria , Bioconversion , Bioreactors , Bottles , Fermentation , Mass transfer , Oxygen , Plant life extension , Soils , Liquids , oxygen , aboveground biomass , bacterium , bioreactor , crop improvement , crop yield , growth regulator , inoculation , maize , mass transfer , microbial activity , oxygen , rhizosphere , article , Azospirillum brasilense , biomass , bioreactor , fermentation , greenhouse , maize , oxygen transport , plant growth , plant-growth promoting bacterium , room temperature , scale up , shelf life , soil inoculation , Azospirillum brasilense , Bacteria (microorganisms) , Zea mays , Azospirillum brasilense , Bacterial Load , Colony Count , Microbial , Industrial Microbiology , Preservation , Biological , Time Factors , Zea mays