Multiple response optimization of Bacillus subtilis EA-CB0015 culture and identification of antifungal metabolites

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dc.citation.epage385spa
dc.citation.issue4spa
dc.citation.journalAbbreviatedTitleISBABspa
dc.citation.journalTitleInternational Society of Biocatalysis and Agricultural Biotechnologyspa
dc.citation.spage378spa
dc.citation.volume3spa
dc.contributor.authorOrdúz, Sergio
dc.contributor.authorMosquera, Sandra
dc.contributor.authorGonzález Jaramillo, Lina María
dc.contributor.authorVillegas Escobar, Valeska
dc.contributor.departmentEscuela de Biociencias
dc.contributor.departmentCentro de Investigaciones del Banano
dc.contributor.departmentUniversidad EAFIT. Departamento de Cienciasspa
dc.contributor.eafitauthorValeska Villegas-Escobar (vvilleg2@eafit.edu.co)spa
dc.contributor.researchgroupCiencias Biológicas y Bioprocesos (CIBIOP)spa
dc.date.accessioned2015-08-13T14:09:15Z
dc.date.available2015-08-13T14:09:15Z
dc.date.issued2014
dc.description.abstractThe low yields of biomass and antimicrobial metabolites obtained in fermentation processes are limiting factors for implementing biological control agents in the field -- In this context, optimization of the culture medium for the biological control agent Bacillus subtilis EA-CB0015 was conducted in submerged culture to maximize the biomass production and antifungal activity -- Additionally, the active metabolites against the phytopathogen Mycosphaerella fijiensis produced under optimized conditions were identified -- Carbon and nitrogen constituents of the culture medium were optimized using a 22 factorial design with central point followed by a multiple response optimization coupled to the desirability function approach -- The optimized medium (33.4 g/L of glucose and 32.5 g/L of yeast extract), showed significant increases in both, cell density by 3.6 folds (13.271.0 g/L) and antifungal activity by 1.2 folds (77.770.3% inhibition) -- To gain insight into the type of active compounds, they were purified and identified by mass spectrometry (MS) -- The MS analysis revealed the presence of three families of lipopeptides: surfactin, fengycin and iturin -- The high antifungal activity was associated with the novel fengycin C and with iturin -- A which were partially growth-associated with a maximum concentration of 781.4 and 355.4 mg/L at 36 h of growth, respectively -- These results indicate the potential of B. subtilis EA-CB0015 to produce high concentrations of biomass and lipopeptides that can be exploited for biotechnological applicationeng
dc.identifier.doi10.1016/j.bcab.2014.09.004spa
dc.identifier.otherWOS;000326394000008
dc.identifier.otherPUBMED;24061414
dc.identifier.otherSCOPUS;2-s2.0-84888435786
dc.identifier.urihttp://hdl.handle.net/10784/7254
dc.language.isoengeng
dc.publisherBiocatalysis and Agricultural Biotechnologyspa
dc.relation.ispartofInternational Society of Biocatalysis and Agricultural Biotechnology(ISBAB), Volume 2, Issue 2, pp 378-385spa
dc.relation.urihttp://www.sciencedirect.com/science/article/pii/S1878818114001157
dc.rights.accessrightsinfo:eu-repo/semantics/closedAccessspa
dc.rights.localAcceso restringidospa
dc.subject.keywordFungal metaboliteseng
dc.subject.keywordBiomasseng
dc.subject.keywordBacillus subtiliseng
dc.subject.keywordFermentationeng
dc.subject.keywordAntifungal agentseng
dc.subject.keywordBiological controleng
dc.subject.keywordFungi as biological pest control agentseng
dc.subject.keywordLipopéptidosspa
dc.subject.keywordEspectometríaspa
dc.subject.keywordSurfactinasspa
dc.subject.keywordBacterias como agentes biológicosspa
dc.subject.lembBACILLUS SUBTILISspa
dc.subject.lembMETABOLITOS FUNGOSOSspa
dc.subject.lembBIOMASAspa
dc.subject.lembFERMENTACIÓNspa
dc.subject.lembAGENTES ANTIFUNGOSOSspa
dc.subject.lembCONTROL BIOLÓGICOspa
dc.subject.lembAGENTES BIOLÓGICOS PARA EL CONTROL DE PLAGASspa
dc.titleMultiple response optimization of Bacillus subtilis EA-CB0015 culture and identification of antifungal metaboliteseng
dc.typearticleeng
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.typepublishedVersioneng
dc.type.localArtículo

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