Evaluación del crecimiento de Spirulina platensis UTEX 1926 cultivada en medios salinos, utilizando CO2 como fuente de carbono
Fecha
2017
Autores
Rojas Osorio, Daniela
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Editor
Universidad EAFIT
Resumen
The cell growth of Spirulina platensis and the production their metabolites, are affected by factors such as the supply of nutrients and physical-chemical parameters such as salinity, pH, temperature and light availability -- The reduction of the use of fresh water and the reduction of production costs of Spirulina platensis culture; the use of sea water is implemented, due to its content of several inorganic constituents that are available to the primaries producers such as microalgae and cyanobacteria -- In this study the cell growth of the Spirulina platensis UTEX 1926 and the production of metabolites under various concentrations of salinity, sodium nitrate and dipotassium phosphate were evaluated in order to obtain the most suitable condition and the understanding of physiological that intervene in the metabolism of cyanobacteria -- A Central Composite Design (CCD) was applied and each treatment was performed thrice -- The strain was cultivated in 1000mL flasks, with an air stream enriched with 7% CO2, photoperiod 12:12 under artificial light (LED) with an intensity of 55±4μmol/m2s and temperature on average 27°C±3 -- The results showed that Spirulina platensis UTEX 1926 reached a cellular concentration of 2197mg/L when concentrations of 38,546g/L, 0,727g/L, 0,855g/L salinity, sodium nitrate and dipotassium phosphate respectively were used -- In addition, under these concentrations of nutrients, 203mg/L of proteins, 4mg/L of phycocyanin and 3mg/L of exopolysaccharides were obtained -- Finally the cost per unit of mass is 0,19US for the chosen condition, being a means of low cost compared to the Zarrouk medium (0,74US per unit mass)