Examinando por Materia "Genetic engineering"

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    La asincronía entre la ciencia y la ética : un reto para la supervivencia
    (Universidad EAFIT, 2023) Escobar Restrepo, Gustavo Adolfo; Vélez López, Germán Darío
    This paper aims to find the state of the matter regarding the solvency of an useful and valid ethical framework to face the unintentional challenges of science, impassive, in particular genetic engineering, impacting humanity’s concept of good and virtue. Assuming different perspectives, some catastrophic, some moderate and some hopeful, the concepts and recommendations of scientists and international entities are reviewed. Furthermore, the perceptions of three contemporary thinkers on the issues generated by the science of genetic engineering and human genome modifications are also reviewed, which open the doors to a potential and new eugenics, both liberal and totalitarian, with the effects on an individual’s self-understanding as well as the ethical understanding and validity of the species. The paper’s hypothesis indicates that there is currently an important asynchrony between science and ethics, stating that there is no ethical capacity leading humanity through time and evolution, seeking the survival of the species or accompanying it on its journey to a new era and a new humanity.
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    Clonación del gen crtI, procedente de Rhodotorula mucilaginosa EA-BIO010 en cepas de Escherichia coli TOP10, mediante técnicas de ingeniería genética
    (Universidad EAFIT, 2023) Ospina Mejía, Manuel; Sierra Zapata, Laura; Villanueva Mejía, Diego Fernando
    Reactive oxygen species are unstable molecules that contain oxygen and react easily with other molecules in the cell where they cause damage to lipid molecules, proteins and DNA. To combat them, antioxidants such as beta-carotene have been identified, produced by organisms such as Blakeslea trispora, Dunalliela salina, Rhodotorula glutinis, Rhodotorula mucilaginosa, among others. However, beta-carotene is produced in low amounts compared to other antioxidants by yeast strains such as R. mucilaginosa. It should be noted that many of the methodologies used today for the extraction of antioxidants from plants or microorganisms are inefficient, expensive and environmentally unsustainable. Yeasts of the genus Rhodotorula then become promising candidates for beta-carotene production, since it has been shown that their beta-carotene producing cluster can be expressed heterologously in other organisms to optimize its production. Therefore, this project aimed to establish the first stage of the methodology for the heterologous production of the biosynthetic cluster of Rhodotorula mucilaginosa EA-BIO010 in E. coli TOP10 strains, starting with the cloning of the crtl gene. This new recombinant micro-organism could be used in the future for the sustainable and efficient production of beta-carotenes and contribute to the creation of biological processes for the production of antioxidants for industrial use.