Examinando por Autor "Trujillo-Roldan, Mauricio A."

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    Ítem
    The role of volumetric power input in the growth, morphology, and production of a recombinant glycoprotein by Streptomyces lividans in shake flasks
    (ELSEVIER SCIENCE BV, 2014-09-15) Marin-Palacio, Luz D.; Gamboa-Suasnavart, Ramses A.; Valdez-Cruz, Norma A.; Servin-Gonzalez, Luis; Soledad Cordova-Aguilar, Ma.; Soto, Enrique; Kloeckner, Wolf; Buechs, Jochen; Trujillo-Roldan, Mauricio A.; Marin-Palacio, Luz D.; Gamboa-Suasnavart, Ramses A.; Valdez-Cruz, Norma A.; Servin-Gonzalez, Luis; Soledad Cordova-Aguilar, Ma.; Soto, Enrique; Kloeckner, Wolf; Buechs, Jochen; Trujillo-Roldan, Mauricio A.; Universidad EAFIT. Departamento de Ingeniería de Procesos; Procesos Ambientales (GIPAB)
    The impact of flask geometry on Streptomyces lividans growth and morphology, production and O-mannosylation of a recombinant O-glycoprotein (APA from Mycobacterium tuberculosis) was described and associated to the evolution of the volumetric power input (P/V) in three shake flask geometries. During the exponential growth, the highest P/V was found in baffled flasks (BF) with 0.51kW/m3, followed by coiled flasks (CF) with 0.44kW/m3 and normal Erlenmeyer flasks (NF) with 0.20kW/m3 (flasks volume of 250mL, filling with 50mL and agitated at 150rpm). During the stationary phase, P/V decreased 20% in BF and CF, but increased two times in NF, surely due to changes in mycelial morphology and its effects on rheology. Also, NF cultures were carried out at a filling volume and agitation of 15mL, 150rpm (15mL-NF), and 25mL, 168rpm (25mL-NF), in order to raise P/V closely to the values obtained in CF. However, different growth, morphology and recombinant protein productivity were obtained. These data indicate that P/V is not a definitive parameter that can determine bacteria growth and morphology, not even glycoprotein production. But it can be proposed that the oxygen transfer in the center of the pellets and hydromechanical stress might be the more relevant parameters than P/V. © 2014 Elsevier B.V.
  • No hay miniatura disponible
    Ítem
    The role of volumetric power input in the growth, morphology, and production of a recombinant glycoprotein by Streptomyces lividans in shake flasks
    (ELSEVIER SCIENCE BV, 2014-09-15) Marin-Palacio, Luz D.; Gamboa-Suasnavart, Ramses A.; Valdez-Cruz, Norma A.; Servin-Gonzalez, Luis; Soledad Cordova-Aguilar, Ma.; Soto, Enrique; Kloeckner, Wolf; Buechs, Jochen; Trujillo-Roldan, Mauricio A.; Universidad EAFIT. Departamento de Ciencias; Ciencias Biológicas y Bioprocesos (CIBIOP)
    The impact of flask geometry on Streptomyces lividans growth and morphology, production and O-mannosylation of a recombinant O-glycoprotein (APA from Mycobacterium tuberculosis) was described and associated to the evolution of the volumetric power input (P/V) in three shake flask geometries. During the exponential growth, the highest P/V was found in baffled flasks (BF) with 0.51kW/m3, followed by coiled flasks (CF) with 0.44kW/m3 and normal Erlenmeyer flasks (NF) with 0.20kW/m3 (flasks volume of 250mL, filling with 50mL and agitated at 150rpm). During the stationary phase, P/V decreased 20% in BF and CF, but increased two times in NF, surely due to changes in mycelial morphology and its effects on rheology. Also, NF cultures were carried out at a filling volume and agitation of 15mL, 150rpm (15mL-NF), and 25mL, 168rpm (25mL-NF), in order to raise P/V closely to the values obtained in CF. However, different growth, morphology and recombinant protein productivity were obtained. These data indicate that P/V is not a definitive parameter that can determine bacteria growth and morphology, not even glycoprotein production. But it can be proposed that the oxygen transfer in the center of the pellets and hydromechanical stress might be the more relevant parameters than P/V. © 2014 Elsevier B.V.
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    Ítem
    Scale-up from shake flasks to pilot-scale production of the plant growth-promoting bacterium Azospirillum brasilense for preparing a liquid inoculant formulation
    (SPRINGER, 2013-11-01) Trujillo-Roldan, Mauricio A.; Valdez-Cruz, Norma A.; Gonzalez-Monterrubio, Cesar F.; Acevedo-Sanchez, Eduardo V.; Martinez-Salinas, Carlos; Garcia-Cabrera, Ramses I.; Gamboa-Suasnavart, Ramses A.; Marin-Palacio, Luz D.; Villegas, Jesus; Blancas-Cabrera, Abel; Trujillo-Roldan, Mauricio A.; Valdez-Cruz, Norma A.; Gonzalez-Monterrubio, Cesar F.; Acevedo-Sanchez, Eduardo V.; Martinez-Salinas, Carlos; Garcia-Cabrera, Ramses I.; Gamboa-Suasnavart, Ramses A.; Marin-Palacio, Luz D.; Villegas, Jesus; Blancas-Cabrera, Abel; Universidad EAFIT. Departamento de Ingeniería de Procesos; Procesos Ambientales (GIPAB)
    Azospirillum brasilense has industrial significance as a growth promoter in plants of commercial interest. However, there is no report in the literature disclosing a liquid product produced in pilot-scale bioreactors and is able to be stored at room temperature for more than 2 years. The aim of this work was to scale up a process from a shake flask to a 10-L lab-scale and 1,000-L pilot-scale bioreactor for the production of plant growth-promoting bacterium A. brasilense for a liquid inoculant formulation. Furthermore, this work aimed to determine the shelf life of the liquid formulation stored at room temperature and to increase maize crops yield in greenhouses. Under a constant oxygen mass transfer coefficient (K L a), a fermentation process was successfully scaled up from shake flasks to 10- and 1,000-L bioreactors. A concentration ranging from 3.5 to 7.5 × 108 CFU/mL was obtained in shake flasks and bioreactors, and after 2 years stored at room temperature, the liquid formulation showed one order of magnitude decrease. Applications of the cultured bacteria in maize yields resulted in increases of up to 95 % in corncobs and 70 % in aboveground biomass. © 2013 Springer-Verlag Berlin Heidelberg.
  • No hay miniatura disponible
    Ítem
    Scale-up from shake flasks to pilot-scale production of the plant growth-promoting bacterium Azospirillum brasilense for preparing a liquid inoculant formulation
    (SPRINGER, 2013-11-01) Trujillo-Roldan, Mauricio A.; Valdez-Cruz, Norma A.; Gonzalez-Monterrubio, Cesar F.; Acevedo-Sanchez, Eduardo V.; Martinez-Salinas, Carlos; Garcia-Cabrera, Ramses I.; Gamboa-Suasnavart, Ramses A.; Marin-Palacio, Luz D.; Villegas, Jesus; Blancas-Cabrera, Abel; Universidad EAFIT. Departamento de Ciencias; Ciencias Biológicas y Bioprocesos (CIBIOP)
    Azospirillum brasilense has industrial significance as a growth promoter in plants of commercial interest. However, there is no report in the literature disclosing a liquid product produced in pilot-scale bioreactors and is able to be stored at room temperature for more than 2 years. The aim of this work was to scale up a process from a shake flask to a 10-L lab-scale and 1,000-L pilot-scale bioreactor for the production of plant growth-promoting bacterium A. brasilense for a liquid inoculant formulation. Furthermore, this work aimed to determine the shelf life of the liquid formulation stored at room temperature and to increase maize crops yield in greenhouses. Under a constant oxygen mass transfer coefficient (K L a), a fermentation process was successfully scaled up from shake flasks to 10- and 1,000-L bioreactors. A concentration ranging from 3.5 to 7.5 × 108 CFU/mL was obtained in shake flasks and bioreactors, and after 2 years stored at room temperature, the liquid formulation showed one order of magnitude decrease. Applications of the cultured bacteria in maize yields resulted in increases of up to 95 % in corncobs and 70 % in aboveground biomass. © 2013 Springer-Verlag Berlin Heidelberg.