Examinando por Autor "Saldamando-Benjumea, C.I."
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Ítem Microsatellite Analysis Reveals Population Structure and Population Expansion of Tecia solanivora in Solanum tuberosum in Colombia(Southwestern Entomological Society, 2015-03-01) Villanueva-Mejía, D.F.; Ramírez-Ríos, V.; Arango-Isaza, R.E.; Saldamando-Benjumea, C.I.; Universidad EAFIT. Departamento de Ciencias; Ciencias Biológicas y Bioprocesos (CIBIOP)Tecia solanivora (Lepidoptera: Gelechiidae) (Povolny 1973), is the most important insect pest of potato (Solanum tuberosum L.) in Central and South America, and Spain. The species was recently reported in Mexico, demonstrating the power of invasion by this insect. In Peru, the insect is quarantined, and could become a damaging pest in America. Larvae attack potato tubers, causing economic loss of 50 to 100%. We used eight microsatellites in 152 individuals from Antioquia (North), Boyaca (Center), Narino (South), and Norte de Santander (East), Colombia, and found T. solanivora genetically different based on an AMOVA test (F-ST = 0.094, P < 0.01, R-ST = 0.175, P < 0.01). The differentiation was supported by Bayesian analysis where we estimated K = 2. The outcome suggests populations from Antioquia and Boyaca are genetically similar whereas populations from Narino and Norte de Santander differ because of geographical separation from other regions. We found T. solanivora had not undergone a recent bottleneck effect suggested by other authors. On the contrary we found that this species has undergone recent population expansion in the country. We suggest that movement of potatoes caused genetic homogenization. Movement occurs from Boyaca where most potatoes are produced, to the rest of Colombia.Ítem Mitochondrial genome characterization of Tecia solanivora (Lepidoptera: Gelechiidae) and its phylogenetic relationship with other lepidopteran insects(Elsevier, 2016-05-01) Ramírez-Ríos, V.; Franco-Sierra, N.D.; Alvarez, J.C.; Saldamando-Benjumea, C.I.; Villanueva, Diego Fernando; Universidad EAFIT. Departamento de Ciencias; Ciencias Biológicas y Bioprocesos (CIBIOP)The complete mitogenome of the potato tuber moth Tecia solanivora (Lepidoptera: Gelechiidae) was sequenced, annotated, characterized and compared with 140 species of the order Lepidoptera. The circular genome is 15,251 bp, containing 37 genes (13 protein-coding genes (PCGs), two rRNA genes, 22 tRNA genes and an A + T-rich region). The gene arrangement was identical to other lepidopteran mitogenomes but different from the ancestral arrangement found in most insects for the tRNA-Met gene (A + T-region, tRNA-I, tRNA-Q, tRNA-M). The mitogenome of T. solanivora is highly A + T-biased (78.2%) and exhibits negative AT- and GC-skews. All PCGs are initiated by canonical ATN start codons, except for Cytochrome Oxidase subunit 1 (COI), which is initiated by CGA. Most PCGs have a complete typical stop codon (TAA). Only NAD1 has a TAG stop codon and the COII and NAD5 genes have an incomplete stop codon consisting of just a T. The A + T-rich region is 332 bp long and contains common features found in lepidopteran mitogenomes, including the `ATAGA' motif, a 17 bp poly (T) stretch and a (AT)(8) element preceded by the `ATTTA' motif. Other tandem repeats like (TAA)(4) and (TAT), were found, as well as (T)(6) and (A)(10) mononucleotide repeat elements. Finally, this mitogenome has 20 intergenic spacer regions. The phylogenetic relationship of T. solanivora with 28 other lepidopteran families (12 superfamilies) showed that taxonomic classification by morphological features coincides with the inferred phylogeny. Thus, the Gelechiidae family represents a monophyletic group, suggesting that T. solanivora and Pectinophora gossypiella have a recent common ancestor. (C) 2016 Elsevier B.V. All rights reserved.Ítem Mitochondrial genome characterization of Tecia solanivora (Lepidoptera: Gelechiidae) and its phylogenetic relationship with other lepidopteran insects(Elsevier, 2016-05-01) Ramírez-Ríos, V.; Franco-Sierra, N.D.; Alvarez, J.C.; Saldamando-Benjumea, C.I.; Villanueva, Diego Fernando; Universidad EAFIT. Departamento de Ciencias; Biodiversidad, Evolución y ConservaciónThe complete mitogenome of the potato tuber moth Tecia solanivora (Lepidoptera: Gelechiidae) was sequenced, annotated, characterized and compared with 140 species of the order Lepidoptera. The circular genome is 15,251 bp, containing 37 genes (13 protein-coding genes (PCGs), two rRNA genes, 22 tRNA genes and an A + T-rich region). The gene arrangement was identical to other lepidopteran mitogenomes but different from the ancestral arrangement found in most insects for the tRNA-Met gene (A + T-region, tRNA-I, tRNA-Q, tRNA-M). The mitogenome of T. solanivora is highly A + T-biased (78.2%) and exhibits negative AT- and GC-skews. All PCGs are initiated by canonical ATN start codons, except for Cytochrome Oxidase subunit 1 (COI), which is initiated by CGA. Most PCGs have a complete typical stop codon (TAA). Only NAD1 has a TAG stop codon and the COII and NAD5 genes have an incomplete stop codon consisting of just a T. The A + T-rich region is 332 bp long and contains common features found in lepidopteran mitogenomes, including the `ATAGA' motif, a 17 bp poly (T) stretch and a (AT)(8) element preceded by the `ATTTA' motif. Other tandem repeats like (TAA)(4) and (TAT), were found, as well as (T)(6) and (A)(10) mononucleotide repeat elements. Finally, this mitogenome has 20 intergenic spacer regions. The phylogenetic relationship of T. solanivora with 28 other lepidopteran families (12 superfamilies) showed that taxonomic classification by morphological features coincides with the inferred phylogeny. Thus, the Gelechiidae family represents a monophyletic group, suggesting that T. solanivora and Pectinophora gossypiella have a recent common ancestor. (C) 2016 Elsevier B.V. All rights reserved.Ítem Phylogeography of Tecia solanivora from Colombia Based on Cytochrome Oxydase I and Cytochrome b Mitochondrial Genes(Southwestern Entomological Society, 2015-12-01) Villanueva-Mejía, D.F.; Ramírez-Ríos, V.; Arango-Lsaza, R.E.; Saldamando-Benjumea, C.I.; Universidad EAFIT. Departamento de Ciencias; Ciencias Biológicas y Bioprocesos (CIBIOP)The Guatemalan potato moth, Tecia solanivora (Povolny) (Lepidoptera: Gelechiidae), is the most important insect pest of potato, Solanum tuberosum L., worldwide. Larvae attack tubers and are difficult to control. The insect has been characterized by microsatellites and the cytochrome b mitochondrial gene. However, the studies used populations from S. tuberosum and no data are available for populations on other species of potatoes. We used mitochondrial gene markers cytochrome oxydase I and cytochrome b to analyze phylogeography of T. solanivora from S. tuberosum and S. phureja from Antioquia, Boyaca, Narino, and Norte de Santander regions of Colombia, demonstrating the species was genetically structured and no genetic differentiation was found between the two hosts. The Tajima-Nei test showed the population from Boyaca where most potatoes are produced in Colombia was the only one with recent expansion. The divergence (expansion) time of T. solanivora populations from Boyaca occurred 180,000 years ago according to mismatch distribution analysis. Sequences of cytochrome b from Genbank from Canary Islands, Colombia, Costa Rica, Ecuador, Guatemala, and Venezuela showed T. solanivora genetically structured as reported elsewhere. Populations under recent expansion are from Colombia, Ecuador, and Venezuela according to the Tajima-Nei test. Mismatch distribution analysis showed divergence in Guatemala occurred 1.5 million years ago, followed by Costa Rica at 1.38 million years, and Venezuela, Ecuador, and Colombia 388,000 years ago. Results coincided with reports of invasion patterns of the species from Central to South America after the species originated in Guatemala.