Examinando por Autor "Franco Leyva, Ana Maria de los Angeles"

Mostrando 1 - 1 de 1
  • No hay miniatura disponible
    Ítem
    Caracterización química y evaluación de la actividad biológica de extractos de Gliricidia sepium con variación latitudinal
    (2018) Franco Leyva, Ana Maria de los Angeles; Marín Palacio, Luz Deisy
    Recently, Bioactive compounds of plants have recently been studied because of their multiple benefits. In this sense, the biological activity of Gliricidia sepium taken from of three different altitudes (0, 800 y 1500 m.a.s.l) of Colombian tropic has been tested. In the present study three solvents (Ethanol, Ethanol-Water and Water) were evaluated for the solid-liquid conventional extraction with two solids charges (1:4 and 1:8) and supercritical CO2 extraction. The highest yields of extraction were obtained with the hydroethanolic extraction treatment and 800 m.a.s.l (273.07± 6.52 mg/g on average). Additionally, phenol, flavonoids and terpenoids were quantified in which the Ethanolic extraction with solid charge of 1:8 and 0 m.a.s.l presented the highest concentrations (79.9±2.5 ppm, 112.1±4.8 ppm y 640.9 ±15.9 ppm respectively). Additionally, the treatment with the highest antioxidant activity (ORAC) corresponded to the one performed with a solid charge of 1:8 in ethanol and 800 m.a.s.l (1919.4 µM de TE/g). At the same time the antioxidant activity results of extraction with supercritical CO2 reached concentration of 677.9; 864.9; 939.6 µM de TE/g 0, 800 Y 1500 m.a.s.l respectively. Finally, the antimicrobial activity of extracts was tested in different microorganisms, the highest inhibition was the one presented against Ralstonia solanacearum EAP09 with inhibition percentage of 90.11±11.20% after 48 hours of exposure. This results permit lead us to the conclusion that ethanolic extract with solids charge 1:8 of G. sepium collected to 800 m.a.s.l present the highest biologic activity, proposing the extracts of the plant as possible source of antioxidant and antimicrobial compounds. Key