Examinando por Autor "Álvarez, J.C."

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    Bacillus subtilis EA-CB0575 genome reveals clues for plant growth promotion and potential for sustainable agriculture
    (Springer, 2020-01-01) Franco-Sierra, N.D.; Posada, L.F.; Santa-María, G.; Romero-Tabarez, M.; Villegas-Escobar, V.; Álvarez, J.C.; Universidad EAFIT. Departamento de Ciencias; Biodiversidad, Evolución y Conservación
    Bacillus subtilis is a remarkably diverse bacterial species that displays many ecological functions. Given its genomic diversity, the strain Bacillus subtilis EA-CB0575, isolated from the rhizosphere of a banana plant, was sequenced and assembled to determine the genomic potential associated with its plant growth promotion potential. The genome was sequenced by Illumina technology and assembled using Velvet 1.2.10, resulting in a whole genome of 4.09 Mb with 4332 genes. Genes involved in the production of indoles, siderophores, lipopeptides, volatile compounds, phytase, bacilibactin, and nitrogenase were predicted by gene annotation or by metabolic pathway prediction by RAST. These potential traits were determined using in vitro biochemical tests, finding that B. subtilis EA-CB0575 produces two families of lipopeptides (surfactin and fengycin), solubilizes phosphate, fixes nitrogen, and produces indole and siderophores compounds. Finally, strain EA-CB0575 increased 34.60% the total dry weight (TDW) of tomato plants with respect to non-inoculated plants at greenhouse level. These results suggest that the identification of strain-specific genes and predicted metabolic pathways might explain the strain potential to promote plant growth by several mechanisms of action, accelerating the development of plant biostimulants for sustainable agricultural. © 2020, Springer-Verlag GmbH Germany, part of Springer Nature.
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    Bacillus subtilis EA-CB0575 genome reveals clues for plant growth promotion and potential for sustainable agriculture
    (Springer, 2020-01-01) Franco-Sierra, N.D.; Posada, L.F.; Santa-María, G.; Romero-Tabarez, M.; Villegas-Escobar, V.; Álvarez, J.C.; Universidad EAFIT. Departamento de Ciencias; Ciencias Biológicas y Bioprocesos (CIBIOP)
    Bacillus subtilis is a remarkably diverse bacterial species that displays many ecological functions. Given its genomic diversity, the strain Bacillus subtilis EA-CB0575, isolated from the rhizosphere of a banana plant, was sequenced and assembled to determine the genomic potential associated with its plant growth promotion potential. The genome was sequenced by Illumina technology and assembled using Velvet 1.2.10, resulting in a whole genome of 4.09 Mb with 4332 genes. Genes involved in the production of indoles, siderophores, lipopeptides, volatile compounds, phytase, bacilibactin, and nitrogenase were predicted by gene annotation or by metabolic pathway prediction by RAST. These potential traits were determined using in vitro biochemical tests, finding that B. subtilis EA-CB0575 produces two families of lipopeptides (surfactin and fengycin), solubilizes phosphate, fixes nitrogen, and produces indole and siderophores compounds. Finally, strain EA-CB0575 increased 34.60% the total dry weight (TDW) of tomato plants with respect to non-inoculated plants at greenhouse level. These results suggest that the identification of strain-specific genes and predicted metabolic pathways might explain the strain potential to promote plant growth by several mechanisms of action, accelerating the development of plant biostimulants for sustainable agricultural. © 2020, Springer-Verlag GmbH Germany, part of Springer Nature.
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    Ceratocystis wilt pathogens: History and biology-highlighting C. Cacaofunesta, the causal agent of wilt disease of cacao
    (Springer International Publishing, 2016-01-01) Cabrera, O.G.; Molano, E.P.L.; José, J.; Álvarez, J.C.; Pereira, G.A.G.
    Ceratocystis is a genus of ascomycete fungi that includes aggressive pathogens of economically important plants worldwide. This fungus is the causal agent of Ceratocystis wilt disease and canker disease, which often kills the plant causing major losses in agricultural production. In the last two decades, emerging diseases related to Ceratocystis infections have been greatly increased. Ceratocystis wilt of cacao is caused by C. cacaofunesta, one of the three well-established host-specific pathogens in the genus. Ceratocystis wilt of cacao has caused sporadic epidemics in the Americas, but its importance is often underestimated. Furthermore, the disease represents a serious threat to the world’s cacao production due to the risk of pathogen spread. Silvicultural practices in cacao agroforests, the marketing of seeds, and cacao grafting in association with a minimal knowledge of the biology of the pathogen effectively contributed to this threat. This chapter explores the controversial taxonomic and evolutionary history of the genus Ceratocystis as well as the biology of C. cacaofunesta. © Springer International Publishing Switzerland 2016.
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    Ceratocystis Wilt Pathogens: History and Biology—Highlighting C. cacaofunesta, the Causal Agent of Wilt Disease of Cacao
    (Springer International Publishing, 2016-01-01) García, Oladys; López, Eddy Patricia; José, Juliana; Álvarez, J.C.
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    DIRECT in vitro REGENERATION OF CASTOR BEAN PLANTS (Ricinus communis) USING EPICOTYLS
    (Univ Feder Uberlandia, 2019-03-15) D.F. Villanueva-Mejía; Álvarez, J.C.; Gil, Alejandro; Restrepo, Martha Catalina; Universidad EAFIT. Departamento de Ciencias; Biodiversidad, Evolución y Conservación
    regeneration protocol for castor bean plant (Ricinus communis) was successfully developed using epicotyl sections obtained from in vitro seedlings.
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    DIRECT in vitro REGENERATION OF CASTOR BEAN PLANTS (Ricinus communis) USING EPICOTYLS
    (Univ Feder Uberlandia, 2019-03-15) D.F. Villanueva-Mejía; Álvarez, J.C.; Gil, Alejandro; Restrepo, Martha Catalina; Universidad EAFIT. Departamento de Ciencias; Ciencias Biológicas y Bioprocesos (CIBIOP)
    regeneration protocol for castor bean plant (Ricinus communis) was successfully developed using epicotyl sections obtained from in vitro seedlings.
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    Enhanced molecular visualization of root colonization and growth promotion by Bacillus subtilis EA-CB0575 in different growth systems
    (Elsevier GmbH, 2018-01-01) Posada, L.F.; Álvarez, J.C.; Romero-Tabarez, M.; de-Bashan, L.; Villegas-Escobar, V.; Universidad EAFIT. Departamento de Ciencias; Biodiversidad, Evolución y Conservación
    Bacillus subtilis EA-CB0575 is a plant growth-promoting bacterium (PGPB) associated with banana and tomato crops. Root colonization is an important trait for PGPB microorganisms and potentiates the bacterial effect related to the mechanisms of plant growth promotion. Therefore, detection of bacterial colonization of roots in different culture systems is important in the study of plant–microorganism interactions. In this study, fluorescent in situ hybridization (FISH) and catalyzed reporter deposition–FISH (CARD–FISH) were evaluated to determine the colonization ability of B. subtilis EA-CB0575 on banana and tomato roots planted on solid and liquid Murashige and Skoog medium (MS(S) and MS(L), respectively) and in soil for tomato plants. Results showed B. subtilis colonization 0–30 days post inoculation for banana and tomato plants in different culture systems with differential distribution of bacterial cells along tomato and banana roots. FISH and CARD–FISH methodologies were both successful in detecting B. subtilis colonies, but CARD–FISH proved to be superior due to its enhanced fluorescence signal. The presence of bacteria correlated with the promotion of plant growth in both plant species, providing clues to relate rhizospheric colonization with improvement in plant growth. FISH and CARD–FISH analysis results suggested the presence of native microbiota on the roots of in vitro banana plants, but not on those of tomato plants. © 2018 Elsevier GmbH
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    Enhanced molecular visualization of root colonization and growth promotion by Bacillus subtilis EA-CB0575 in different growth systems
    (Elsevier GmbH, 2018-01-01) Posada, L.F.; Álvarez, J.C.; Romero-Tabarez, M.; de-Bashan, L.; Villegas-Escobar, V.; Universidad EAFIT. Departamento de Ciencias; Ciencias Biológicas y Bioprocesos (CIBIOP)
    Bacillus subtilis EA-CB0575 is a plant growth-promoting bacterium (PGPB) associated with banana and tomato crops. Root colonization is an important trait for PGPB microorganisms and potentiates the bacterial effect related to the mechanisms of plant growth promotion. Therefore, detection of bacterial colonization of roots in different culture systems is important in the study of plant–microorganism interactions. In this study, fluorescent in situ hybridization (FISH) and catalyzed reporter deposition–FISH (CARD–FISH) were evaluated to determine the colonization ability of B. subtilis EA-CB0575 on banana and tomato roots planted on solid and liquid Murashige and Skoog medium (MS(S) and MS(L), respectively) and in soil for tomato plants. Results showed B. subtilis colonization 0–30 days post inoculation for banana and tomato plants in different culture systems with differential distribution of bacterial cells along tomato and banana roots. FISH and CARD–FISH methodologies were both successful in detecting B. subtilis colonies, but CARD–FISH proved to be superior due to its enhanced fluorescence signal. The presence of bacteria correlated with the promotion of plant growth in both plant species, providing clues to relate rhizospheric colonization with improvement in plant growth. FISH and CARD–FISH analysis results suggested the presence of native microbiota on the roots of in vitro banana plants, but not on those of tomato plants. © 2018 Elsevier GmbH
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    Estado de la moniliasis del cacao causada por Moniliophthora roreri en Colombia
    (Universidad Nacional de Colombia, 2014-01-01) Álvarez, J.C.; Martínez, S.C.; Coy, J.; Universidad EAFIT. Departamento de Ciencias; Ciencias Biológicas y Bioprocesos (CIBIOP)
    Frosty pod rot disease of cocoa plants is caused by the basiodiomycete Moniliophthora roreri. Nowadays, this disease is present in almost all Latin American countries producers of cocoa beans, exhibiting high adaptation to diverse environments. In Colombia, it is the most important disease attacking cocoa crops and the traditional strategies for control have generated side results as strengthening in genetic resistance of strains in some regions. In this review, we collected the most relevant biological and agricultural aspects of this disease such as origin and distribution of the disease, life cycle, forms of disease control and research projects oriented to understand the genetic and evolution of this pathogen. Finally, we suggest increasing the number of basic researches, aiming to understand, how this pathogen has evolved its genome in different habitats, favoring its genetic variation. Thus, with all this knowledge, we could advance in biotechnology programs for control and prevention of the Frosty rod pot.
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    Prospecting for lytic bacteriophages against diverse strains of Ralstonia solanacearum from Musa spp. in Colombia.
    (Asociación Colombiana de Fitopatología y Ciencias Afines ASCOLFI, 2016-01-01) Villegas, V.; Álvarez, J.C.; Universidad EAFIT. Departamento de Ciencias; Ciencias Biológicas y Bioprocesos (CIBIOP)
    Bacterial wilt caused by the soilborne pathogen Ralstonia solanacearum is one of the most devastating plant diseases worldwide. In Colombia, this disease causes high economic losses in plantations of banana