The role of volumetric power input in the growth, morphology, and production of a recombinant glycoprotein by Streptomyces lividans in shake flasks

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dc.citation.epage233spa
dc.citation.journalTitleBiochemical Engineering Journalspa
dc.citation.spage224spa
dc.citation.volume90spa
dc.contributor.authorGamboa Suasnavart, Ramsés A
dc.contributor.authorServín González, Luis
dc.contributor.authorSoto, Enrique
dc.contributor.authorKlöckner, Wolf
dc.contributor.authorBüchs, Jochen
dc.contributor.authorTrujillo Roldán, Mauricio A
dc.contributor.authorMarín Palacio, Luz Deisy
dc.contributor.authorValdez Cruz, Norma Adriana
dc.contributor.authorCórdova Aguilar, María Soledad
dc.contributor.departmentUnidad de Bioprocesos, Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México
dc.contributor.departmentCentro de Ciencias Aplicadas y Desarrollo Tecnológico, Universidad Nacional Autónoma de México
dc.contributor.departmentAVT–Biochemical Engineering, RWTH Aachen University
dc.contributor.departmentDepartamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México
dc.contributor.departmentUniversidad EAFIT. Departamento de Cienciasspa
dc.contributor.eafitauthorMauricio A TrujilloRoldán (maurotru@gmail.com)spa
dc.contributor.researchgroupCiencias Biológicas y Bioprocesos (CIBIOP)spa
dc.date.accessioned2015-08-13T14:21:16Z
dc.date.available2015-08-13T14:21:16Z
dc.date.issued2014
dc.description.abstractThe impact of flask geometry on Streptomyces lividans growth and morphology, production and Omannosylation of a recombinant O-glycoprotein (APA from Mycobacterium tuberculosis) was described and associated to the evolution of the volumetric power input (P/V) in three shake flask geometries -- During the exponential growth, the highest P/V was found in baffled flasks (BF) with 0.51 kW/m3, followed by coiled flasks (CF) with 0.44 kW/m3 and normal Erlenmeyer flasks (NF) with 0.20 kW/m3 (flasks volume of 250 mL, filling with 50 mL and agitated at 150 rpm) -- During the stationary phase, P/V decreased 20% in BF and CF, but increased two times in NF, surely due to changes in mycelial morphology and its effects on rheology -- Also, NF cultures were carried out at a filling volume and agitation of 15 mL, 150 rpm (15 mL-NF), and 25 mL, 168 rpm (25 mL-NF), in order to raise P/V closely to the values obtained in CF -- However, different growth, morphology and recombinant protein productivity were obtained -- These data indicate that P/V is not a definitive parameter that can determine bacteria growth and morphology, not even glycoprotein production -- But it can be proposed that the oxygen transfer in the center of the pellets and hydromechanical stress might be the more relevant parameters than P/Veng
dc.identifier.doi10.1016/j.bej.2014.06.010spa
dc.identifier.issn1369-703Xspa
dc.identifier.otherWOS;000370585700033
dc.identifier.otherPUBMED;26760242
dc.identifier.otherSCOPUS;2-s2.0-84952837330
dc.identifier.urihttp://hdl.handle.net/10784/7257
dc.language.isoengeng
dc.publisherElsevierspa
dc.relation.ispartofBiochemical Engineering Journal , Volume 90spa
dc.relation.urihttp://dx.doi.org/10.1016/j.bej.2014.06.010
dc.rightsUnleashing the power of academic sharing - Sharing Policy - Sharing and Hosting Policy FAQ - Green open access - Journal Embargo Period List (pdf) - Journal Embargo List for UK Authors, Attaching a User License (pdf) - Funding Body Agreementsspa
dc.rights.accessrightsinfo:eu-repo/semantics/restrictedAccessspa
dc.rights.localAcceso restringidospa
dc.subject.keywordGlycoproteinseng
dc.subject.keywordRheology (Biology)eng
dc.subject.keywordBioreactorseng
dc.subject.keywordMetaboliteseng
dc.subject.keywordRecombinant proteinseng
dc.subject.keywordGeometría del Matrazspa
dc.subject.keywordMorfología micelialspa
dc.subject.lembGLUCOPROTEÍNASspa
dc.subject.lembREOLOGÍA (BIOLOGÍA)spa
dc.subject.lembBIOREACTORESspa
dc.subject.lembMETABOLITOSspa
dc.subject.lembPROTEÍNAS RECOMBINANTESspa
dc.subject.lembINOCULACIÓNspa
dc.titleThe role of volumetric power input in the growth, morphology, and production of a recombinant glycoprotein by Streptomyces lividans in shake flaskseng
dc.typearticleeng
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.typepublishedVersioneng
dc.type.localArtículo

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