Enhanced molecular visualization of root colonization and growth promotion by Bacillus subtilis EA-CB0575 in different growth systems

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dc.citation.journalTitleMICROBIOLOGICAL RESEARCH
dc.contributor.authorPosada, L.F.
dc.contributor.authorÁlvarez, J.C.
dc.contributor.authorRomero-Tabarez, M.
dc.contributor.authorde-Bashan, L.
dc.contributor.authorVillegas-Escobar, V.
dc.contributor.departmentUniversidad EAFIT. Departamento de Cienciasspa
dc.contributor.researchgroupBiodiversidad, Evolución y Conservaciónspa
dc.date.accessioned2021-03-23T19:52:08Z
dc.date.available2021-03-23T19:52:08Z
dc.date.issued2018-01-01
dc.description.abstractBacillus subtilis EA-CB0575 is a plant growth-promoting bacterium (PGPB) associated with banana and tomato crops. Root colonization is an important trait for PGPB microorganisms and potentiates the bacterial effect related to the mechanisms of plant growth promotion. Therefore, detection of bacterial colonization of roots in different culture systems is important in the study of plant–microorganism interactions. In this study, fluorescent in situ hybridization (FISH) and catalyzed reporter deposition–FISH (CARD–FISH) were evaluated to determine the colonization ability of B. subtilis EA-CB0575 on banana and tomato roots planted on solid and liquid Murashige and Skoog medium (MS(S) and MS(L), respectively) and in soil for tomato plants. Results showed B. subtilis colonization 0–30 days post inoculation for banana and tomato plants in different culture systems with differential distribution of bacterial cells along tomato and banana roots. FISH and CARD–FISH methodologies were both successful in detecting B. subtilis colonies, but CARD–FISH proved to be superior due to its enhanced fluorescence signal. The presence of bacteria correlated with the promotion of plant growth in both plant species, providing clues to relate rhizospheric colonization with improvement in plant growth. FISH and CARD–FISH analysis results suggested the presence of native microbiota on the roots of in vitro banana plants, but not on those of tomato plants. © 2018 Elsevier GmbHeng
dc.formatapplication/pdf
dc.identifierhttps://eafit.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=8198
dc.identifier.doi10.1016/j.micres.2018.08.017
dc.identifier.issn09445013
dc.identifier.issn16180623
dc.identifier.otherWOS;000451654900007
dc.identifier.otherPUBMED;30384910
dc.identifier.otherSCOPUS;2-s2.0-85053471614
dc.identifier.urihttp://hdl.handle.net/10784/26739
dc.language.isoengeng
dc.publisherElsevier GmbH
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85053471614&doi=10.1016%2fj.micres.2018.08.017&partnerID=40&md5=1188ac8fc133d0eed23a60c1c807ddd0
dc.rightshttps://v2.sherpa.ac.uk/id/publication/issn/0944-5013
dc.rights.accessrightsinfo:eu-repo/semantics/openAccesseng
dc.rights.localAcceso abiertospa
dc.sourceMICROBIOLOGICAL RESEARCH
dc.subjectActivationeng
dc.subjectanalysiseng
dc.subjectBacteriologyeng
dc.subjectCatalysiseng
dc.subjectDepositioneng
dc.subjectFisheng
dc.subjectFluorescenceeng
dc.subjectFluorescenceeng
dc.subjectmicroscopyeng
dc.subjectFruitseng
dc.subjectPlantseng
dc.subject(botany)eng
dc.subjectBacilluseng
dc.subjectspeng
dc.subjectFluorescenceeng
dc.subjectineng
dc.subjectsitueng
dc.subjecthybridizationeng
dc.subjectFluorescenteng
dc.subjectineng
dc.subjectsitueng
dc.subjecthybridizationeng
dc.subjectMoleculareng
dc.subjectvisualizationeng
dc.subjectMurashigeeng
dc.subjectandeng
dc.subjectSkoogeng
dc.subjectmediumseng
dc.subjectPlanteng
dc.subjectgrowtheng
dc.subjectpromotingeng
dc.subjectbacteriumeng
dc.subjectPlanteng
dc.subjectgrowtheng
dc.subjectpromotioneng
dc.subjectRooteng
dc.subjectcolonizationeng
dc.subjectBacteriaeng
dc.subjectbacteriumeng
dc.subjectcelleng
dc.subjectcolonyeng
dc.subjectgeneticeng
dc.subjectanalysiseng
dc.subjectgrowtheng
dc.subjectherbeng
dc.subjectmicrobialeng
dc.subjectactivityeng
dc.subjectmoleculareng
dc.subjectanalysiseng
dc.subjectplanteng
dc.subjectrhizosphereeng
dc.subjectrooteng
dc.subjectcolonizationeng
dc.subjectrooteng
dc.subjectsystemeng
dc.subjectBacilluseng
dc.subjectsp.eng
dc.subjectBacilluseng
dc.subjectsubtiliseng
dc.subjectBacteriaeng
dc.subject(microorganisms)eng
dc.subjectLycopersiconeng
dc.subjectesculentumeng
dc.subjectagriculturaleng
dc.subjectinoculationeng
dc.subjectBacilluseng
dc.subjectsubtiliseng
dc.subjectfluorescenceeng
dc.subjectineng
dc.subjectsitueng
dc.subjecthybridizationeng
dc.subjectgrowth,eng
dc.subjectdevelopmenteng
dc.subjectandeng
dc.subjectagingeng
dc.subjectmicrobiologyeng
dc.subjectmicrofloraeng
dc.subjectMusaeng
dc.subjectphysiologyeng
dc.subjectplanteng
dc.subjectdevelopmenteng
dc.subjectplanteng
dc.subjectrooteng
dc.subjectplanteng
dc.subjectseedeng
dc.subjectrhizosphereeng
dc.subjectscanningeng
dc.subjectelectroneng
dc.subjectmicroscopyeng
dc.subjectsoileng
dc.subjecttomatoeng
dc.subjectAgriculturaleng
dc.subjectInoculantseng
dc.subjectBacilluseng
dc.subjectsubtiliseng
dc.subjectIneng
dc.subjectSitueng
dc.subjectHybridization,eng
dc.subjectFluorescenceeng
dc.subjectLycopersiconeng
dc.subjectesculentumeng
dc.subjectMicrobiotaeng
dc.subjectMicroscopy,eng
dc.subjectElectron,eng
dc.subjectScanningeng
dc.subjectMusaeng
dc.subjectPeng
dc.titleEnhanced molecular visualization of root colonization and growth promotion by Bacillus subtilis EA-CB0575 in different growth systemseng
dc.typepublishedVersioneng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.typearticleeng
dc.typeinfo:eu-repo/semantics/articleeng
dc.type.localArtículospa

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