Digital lensless holographic microscopy: numerical simulation and reconstruction with ImageJ

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dc.citation.journalTitleApplied Opticseng
dc.contributor.authorTrujillo, Carlos
dc.contributor.authorPiedrahita-Quintero, Pablo
dc.contributor.authorGarcia-Sucerquia, Jorge
dc.contributor.departmentUniversidad EAFIT. Departamento de Ciencias Básicasspa
dc.contributor.researchgroupÓptica Aplicadaspa
dc.date.accessioned2021-04-12T14:18:45Z
dc.date.available2021-04-12T14:18:45Z
dc.date.issued2020-07-01
dc.description.abstractThe description and validation of an ImageJ open-source plugin to numerically simulate and reconstruct digital lensless holographic microscopy (DLHM) holograms are presented. Two modules compose the presented plugin: the simulation module implements a discrete version of the Rayleigh-Somerfield diffraction formula, which allows the user to directly build a simulated hologram from a known phase and/or amplitude object by just introducing the geometry parameters of the simulated setup; the plugin's reconstruction module implements a discrete version of the Kirchhoff-Helmholtz diffraction integral, thus allowing the user to reconstruct DLHM holograms by introducing the parameters of the acquisition setup and the desired reconstruction distance. The plugin offers the two said modules within the robust environment provided by a complete set of built-in tools for image processing available in ImageJ. While the simulation module has been validated through the evaluation of the forecasted lateral resolution of a DLHM setup in terms of the numerical aperture, the reconstruction module is tested by means of reconstructing experimental DLHM holograms of biological samples. (C) 2020 Optical Society of Americaeng
dc.identifierhttps://eafit.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=11904
dc.identifier.doi10.1364/AO.395672
dc.identifier.issn1559128X
dc.identifier.issn21553165
dc.identifier.otherWOS;000547520400022
dc.identifier.otherPUBMED;32609706
dc.identifier.otherSCOPUS;2-s2.0-85087528166
dc.identifier.urihttp://hdl.handle.net/10784/28007
dc.languageeng
dc.language.isoengeng
dc.publisherOPTICAL SOC AMER
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85087528166&doi=10.1364%2fAO.395672&partnerID=40&md5=0b7b0ee00a62a4543097f0d434218110
dc.rightshttps://v2.sherpa.ac.uk/id/publication/issn/1559-128X
dc.sourceApplied Optics
dc.subject.keywordQuantitative Phaseeng
dc.subject.keywordResolutioneng
dc.titleDigital lensless holographic microscopy: numerical simulation and reconstruction with ImageJeng
dc.typeinfo:eu-repo/semantics/articleeng
dc.typearticleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.typepublishedVersioneng
dc.type.localArtículospa

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