Efficient direct shoot organogenesis and genetic stability in micropropagated sacha inchi (Plukenetia volubilis L.)

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dc.citation.journalTitleBMC Research Notes
dc.contributor.authorRestrepo-Osorio C.
dc.contributor.authorGil-Correal A.
dc.contributor.authorChamorro-Gutiérrez L.
dc.contributor.authorRamírez-Ríos V.
dc.contributor.authorÁlvarez J.C.
dc.contributor.authorVillanueva-Mejía D.
dc.contributor.departmentUniversidad EAFIT. Departamento de Cienciasspa
dc.contributor.researchgroupBiodiversidad, Evolución y Conservaciónspa
dc.date.accessioned2021-03-23T19:52:10Z
dc.date.available2021-03-23T19:52:10Z
dc.date.issued2020-09-03
dc.description.abstractObjective: It is necessary to improve biotech platforms based on in vitro cell tissue culture to support sacha inchi (Plukenetia volubilis L.) research programs and draw on the nutritional value of the high polyunsaturated fatty acid content of its oilseed. Here, we developed a rapid and efficient method for induction and direct in vitro shoot development for this species. Results: Shoots were generated from hypocotyl explants. The highest organogenic response was obtained in woody plant medium supplemented with 1 mg/L thidiazuron and 0.5 mg/L zeatin supplemented with L-glutamine, adenine hemisulfate, and L-arginine. Shoots obtained using this medium were transferred and subcultivated with different concentrations of indole-3-butyric acid and 1-naphthylacetic acid for rooting. For the first time, a histological analysis was performed supporting direct organogenic development in this species. The plantlets obtained were transferred ex vitro with a survival percentage of 80%. The genetic stability of the plants recovered was confirmed by randomly amplified polymorphic DNA analysis. All results indicate that it would be possible to stimulate direct shoot formation from hypocotyls to support the sustainable use of this species. © 2020 The Author(s).eng
dc.formatapplication/pdf
dc.identifierhttps://eafit.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=12169
dc.identifier.doi10.1186/s13104-020-05257-1
dc.identifier.issn17560500
dc.identifier.otherPUBMED;32883361
dc.identifier.otherSCOPUS;2-s2.0-85090319012
dc.identifier.urihttp://hdl.handle.net/10784/26758
dc.language.isoengeng
dc.publisherBioMed Central Ltd.
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85090319012&doi=10.1186%2fs13104-020-05257-1&partnerID=40&md5=17338532a6b5413b5901617efe0d4479
dc.rightshttps://v2.sherpa.ac.uk/id/publication/issn/1756-0500
dc.rights.accessrightsinfo:eu-repo/semantics/openAccesseng
dc.rights.localAcceso abiertospa
dc.sourceBMC Research Notes
dc.subjectAcclimatizationeng
dc.subjectAdventitiouseng
dc.subjectshootseng
dc.subjectOilseedeng
dc.subjectRootingeng
dc.subjectTissueeng
dc.subjectcultureeng
dc.titleEfficient direct shoot organogenesis and genetic stability in micropropagated sacha inchi (Plukenetia volubilis L.)eng
dc.typepublishedVersioneng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.typearticleeng
dc.typeinfo:eu-repo/semantics/articleeng
dc.type.localArtículospa

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