Scale-up from shake flasks to bioreactor, based on power input and Streptomyces lividans morphology, for the production of recombinant APA (45/47 kDa protein) from Mycobacterium tuberculosis.

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dc.citation.journalTitleWORLD J MICROB BIOT
dc.contributor.authorGamboa-Suasnavart RA
dc.contributor.authorMarín-Palacio LD
dc.contributor.authorMartínez-Sotelo JA
dc.contributor.authorEspitia C
dc.contributor.authorServín-González L
dc.contributor.authorValdez-Cruz NA
dc.contributor.authorTrujillo-Roldán MA
dc.contributor.departmentUniversidad EAFIT. Departamento de Ingeniería de Procesosspa
dc.contributor.researchgroupProcesos Ambientales (GIPAB)spa
dc.creatorGamboa-Suasnavart RA
dc.creatorMarín-Palacio LD
dc.creatorMartínez-Sotelo JA
dc.creatorEspitia C
dc.creatorServín-González L
dc.creatorValdez-Cruz NA
dc.creatorTrujillo-Roldán MA
dc.date.accessioned2021-04-16T20:27:38Z
dc.date.available2021-04-16T20:27:38Z
dc.date.issued2013-08-01
dc.description.abstractCulture conditions in shake flasks affect filamentous Streptomyces lividans morphology, as well the productivity and O-mannosylation of recombinant Ala-Pro-rich O-glycoprotein (known as the 45/47 kDa or APA antigen) from Mycobacterium tuberculosis. In order to scale up from previous reported shake flasks to bioreactor, data from the literature on the effect of agitation on morphology of Streptomyces strains were used to obtain gassed volumetric power input values that can be used to obtain a morphology of S. lividans in bioreactor similar to the morphology previously reported in coiled/baffled shake flasks by our group. Morphology of S. lividans was successfully scaled-up, obtaining similar mycelial sizes in both scales with diameters of 0.21 ± 0.09 mm in baffled and coiled shake flasks, and 0.15 ± 0.01 mm in the bioreactor. Moreover, the specific growth rate was successfully scaled up (0.09 ± 0.02 and 0.12 ± 0.01 h(-1), for bioreactors and flasks, respectively), and the recombinant protein productivity measured by densitometry, as well. More interestingly, the quality of the recombinant glycoprotein measured as the amount of mannoses attached to the C-terminal of APA was also scaled- up; with up to five mannose residues in cultures carried out in shake flasks; and six in the bioreactor. However, final biomass concentration was not similar, indicating that although the process can be scaled-up using the power input, others factors like oxygen transfer rate, tip speed or energy dissipation/circulation function can be an influence on bacterial metabolism.eng
dc.identifierhttps://eafit.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=5617
dc.identifier.doi10.1007/s11274-013-1305-5
dc.identifier.issn09593993
dc.identifier.issn15730972
dc.identifier.otherWOS;000321977300009
dc.identifier.otherPUBMED;23475258
dc.identifier.urihttp://hdl.handle.net/10784/29396
dc.language.isoeng
dc.publisherKluwer Academic Publishers
dc.rightshttps://v2.sherpa.ac.uk/id/publication/issn/0959-3993
dc.sourceWORLD J MICROB BIOT
dc.titleScale-up from shake flasks to bioreactor, based on power input and Streptomyces lividans morphology, for the production of recombinant APA (45/47 kDa protein) from Mycobacterium tuberculosis.eng
dc.typeinfo:eu-repo/semantics/articleeng
dc.typearticleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.typepublishedVersioneng
dc.type.localArtículospa

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