Ceratocystis cacaofunesta genome analysis reveals a large expansion of extracellular phosphatidylinositol-specific phospholipase-C genes (PI-PLC)

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dc.citation.journalTitleBmc Genomics
dc.contributor.authorMolano, E.P.L.
dc.contributor.authorCabrera, O.G.
dc.contributor.authorJose, J.
dc.contributor.authordo Nascimento, L.C.
dc.contributor.authorCarazzolle, M.F.
dc.contributor.authorTeixeira, P.J.P.L.
dc.contributor.authorAlvarez, J.C.
dc.contributor.authorTiburcio, R.A.
dc.contributor.authorTokimatu Filho, P.M.
dc.contributor.authorde Lima, G.M.A.
dc.contributor.authorGuido, R.V.C.
dc.contributor.authorCorrêa, T.L.R.
dc.contributor.authorLeme, A.F.P.
dc.contributor.authorMieczkowski, P.
dc.contributor.authorPereira, G.A.G.
dc.contributor.departmentUniversidad EAFIT. Departamento de Cienciasspa
dc.contributor.researchgroupBiodiversidad, Evolución y Conservaciónspa
dc.date.accessioned2021-03-23T19:52:08Z
dc.date.available2021-03-23T19:52:08Z
dc.date.issued2018-01-17
dc.description.abstractBackground: The Ceratocystis genus harbors a large number of phytopathogenic fungi that cause xylem parenchyma degradation and vascular destruction on a broad range of economically important plants. Ceratocystis cacaofunesta is a necrotrophic fungus responsible for lethal wilt disease in cacao. The aim of this work is to analyze the genome of C. cacaofunesta through a comparative approach with genomes of other Sordariomycetes in order to better understand the molecular basis of pathogenicity in the Ceratocystis genus. Results: We present an analysis of the C. cacaofunesta genome focusing on secreted proteins that might constitute pathogenicity factors. Comparative genome analyses among five Ceratocystidaceae species and 23 other Sordariomycetes fungi showed a strong reduction in gene content of the Ceratocystis genus. However, some gene families displayed a remarkable expansion, in particular, the Phosphatidylinositol specific phospholipases-C (PI-PLC) family. Also, evolutionary rate calculations suggest that the evolution process of this family was guided by positive selection. Interestingly, among the 82 PI-PLCs genes identified in the C. cacaofunesta genome, 70 genes encoding extracellular PI-PLCs are grouped in eight small scaffolds surrounded by transposon fragments and scars that could be involved in the rapid evolution of the PI-PLC family. Experimental secretome using LC-MS/MS validated 24% (86 proteins) of the total predicted secretome (342 proteins), including four PI-PLCs and other important pathogenicity factors. Conclusion: Analysis of the Ceratocystis cacaofunesta genome provides evidence that PI-PLCs may play a role in pathogenicity. Subsequent functional studies will be aimed at evaluating this hypothesis. The observed genetic arsenals, together with the analysis of the PI-PLC family shown in this work, reveal significant differences in the Ceratocystis genome compared to the classical vascular fungi, Verticillium and Fusarium. Altogether, our analyses provide new insights into the evolution and the molecular basis of plant pathogenicity. © 2018 The Author(s).eng
dc.formatapplication/pdf
dc.identifierhttps://eafit.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=7940
dc.identifier.doi10.1186/s12864-018-4440-4
dc.identifier.issn14712164
dc.identifier.otherWOS;000422884000002
dc.identifier.otherPUBMED;29343217
dc.identifier.otherSCOPUS;2-s2.0-85040746089
dc.identifier.urihttp://hdl.handle.net/10784/26737
dc.language.isoengeng
dc.publisherBioMed Central Ltd.
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85040746089&doi=10.1186%2fs12864-018-4440-4&partnerID=40&md5=6058b6aedd5783c9f58d4dd67875f57a
dc.rightshttps://v2.sherpa.ac.uk/id/publication/issn/1471-2164
dc.rights.accessrightsinfo:eu-repo/semantics/openAccesseng
dc.rights.localAcceso abiertospa
dc.sourceBmc Genomics
dc.subjectphosphatidylinositoleng
dc.subject4,5eng
dc.subjectbisphosphateeng
dc.subjectphosphodiesteraseeng
dc.subjectvirulenceeng
dc.subjectfactoreng
dc.subjectfungaleng
dc.subjectproteineng
dc.subjectphosphatidylinositoleng
dc.subjectphosphatidylinositoleng
dc.subject4,5eng
dc.subjectbisphosphateeng
dc.subjectphosphodiesteraseeng
dc.subjectArticleeng
dc.subjectCeratocystiseng
dc.subjectCeratocystiseng
dc.subjectcacaofunestaeng
dc.subjectevolutionaryeng
dc.subjectrateeng
dc.subjectfungaleng
dc.subjectgeneeng
dc.subjectfungaleng
dc.subjectgenomeeng
dc.subjectfungaleng
dc.subjectvirulenceeng
dc.subjectFusariumeng
dc.subjectliquideng
dc.subjectchromatography-masseng
dc.subjectspectrometryeng
dc.subjectnaturaleng
dc.subjectselectioneng
dc.subjectnonhumaneng
dc.subjectplanteng
dc.subjectfunguseng
dc.subjectinteractioneng
dc.subjectpositiveeng
dc.subjectselectioneng
dc.subjectproteineng
dc.subjecthydrolysiseng
dc.subjectproteineng
dc.subjectsecretioneng
dc.subjectSordariomyceteseng
dc.subjecttransposoneng
dc.subjectVerticilliumeng
dc.subjectAscomyceteseng
dc.subjectcacaoeng
dc.subjectchemistryeng
dc.subjectgeneeng
dc.subjectexpressioneng
dc.subjectregulationeng
dc.subjectgeneticseng
dc.subjectgenomicseng
dc.subjectmetabolismeng
dc.subjectmicrobiologyeng
dc.subjectmoleculareng
dc.subjectevolutioneng
dc.subjectphylogenyeng
dc.subjectprocedureseng
dc.subjectproteineng
dc.subjectconformationeng
dc.subjectAscomycotaeng
dc.subjectCacaoeng
dc.subjectEvolution,eng
dc.subjectMoleculareng
dc.subjectFungaleng
dc.subjectProteinseng
dc.subjectGeneeng
dc.subjectExpressioneng
dc.subjectRegulation,eng
dc.subjectPlanteng
dc.subjectGenome,eng
dc.subjectFungaleng
dc.subjectGenomicseng
dc.subjectPhosphatidylinositolseng
dc.subjectPhosphoinositideeng
dc.subjectPhospholipaseeng
dc.subjectCeng
dc.subjectPhylogenyeng
dc.subjectProteineng
dc.subjectConformationeng
dc.titleCeratocystis cacaofunesta genome analysis reveals a large expansion of extracellular phosphatidylinositol-specific phospholipase-C genes (PI-PLC)eng
dc.typepublishedVersioneng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.typearticleeng
dc.typeinfo:eu-repo/semantics/articleeng
dc.type.localArtículospa

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