Volumetric power input as a reliable parameter for scale-up from shake flask to stirred-tank bioreactor: Production of a recombinant glycoprotein by Streptomyces lividans

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dc.citation.journalTitleREV MEX ING QUIM
dc.contributor.authorGamboa-Suasnavart R.A.
dc.contributor.authorMarín-Palacio L.D.
dc.contributor.authorLópez-Griego L.
dc.contributor.authorCórdova-Aguilar M.S.
dc.contributor.authorValdez-Cruz N.A.
dc.contributor.authorTrujillo-Roldán M.A.
dc.contributor.departmentUniversidad EAFIT. Departamento de Ingeniería de Procesosspa
dc.contributor.researchgroupProcesos Ambientales (GIPAB)spa
dc.creatorGamboa-Suasnavart R.A.
dc.creatorMarín-Palacio L.D.
dc.creatorLópez-Griego L.
dc.creatorCórdova-Aguilar M.S.
dc.creatorValdez-Cruz N.A.
dc.creatorTrujillo-Roldán M.A.
dc.date.accessioned2021-04-16T20:27:39Z
dc.date.available2021-04-16T20:27:39Z
dc.date.issued2019-01-01
dc.description.abstractThe filamentous morphology of Streptomyces lividans depends on the culture conditions, affecting the production, secretion and post-translational modifications of recombinant glycoproteins. In this work, the previously reported volumetric power input (P/V) in conventional (NF) and coiled (CF) shake flasks were scaled-up to a stirred bioreactor. The effects on the growth and morphology of S. lividans were analyzed, as well as, the production and O-mannosylation of the recombinant APA glycoprotein from Mycobacterium tuberculosis. Specific growth rates of 5. lividans and similar recombinant glycoprotein (rAPA) yields were observed between NF and bioreactor cultures. In addition, we have found up to seven mannose residues attached to the C-terminal of the rAPA in bioreactor cultures, one more than in NF and CF. However, at similar P/V values, morphological and kinetic differences were found. Our data indicate that P/V as scale-up criteria in the production of recombinant glycoproteins in S. lividans can be successful in some, but not all the kinetic and stoichiometric parameters, suggesting that the metabolic cell responses can be affected by aeration/hydrodynamics between bioreactor and shake flasks. © 2019, Universidad Autonoma Metropolitana Iztapalapa. All rights reserved.eng
dc.identifierhttps://eafit.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=9855
dc.identifier.doi10.24275/uam/izt/dcbi/revmexingquim/2019v18n3/Gamboa
dc.identifier.issn16652738
dc.identifier.otherSCOPUS;2-s2.0-85071226208
dc.identifier.urihttp://hdl.handle.net/10784/29413
dc.language.isospa
dc.publisherUniversidad Autonoma Metropolitana-Iztapalapa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85071226208&doi=10.24275%2fuam%2fizt%2fdcbi%2frevmexingquim%2f2019v18n3%2fGamboa&partnerID=40&md5=f549f85ce514fb1e51ba22e5fe5a5f89
dc.rightsUniversidad Autonoma Metropolitana-Iztapalapa
dc.sourceREV MEX ING QUIM
dc.subjectMorphologyeng
dc.subjectPower inputeng
dc.subjectRheologyeng
dc.subjectS. lividanseng
dc.subjectScale-upeng
dc.subjectShake flaskseng
dc.titleVolumetric power input as a reliable parameter for scale-up from shake flask to stirred-tank bioreactor: Production of a recombinant glycoprotein by Streptomyces lividanseng
dc.typeinfo:eu-repo/semantics/articleeng
dc.typearticleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.typepublishedVersioneng
dc.type.localArtículospa

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