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dc.date.available2015-08-13T14:21:16Z
dc.date.issued2014
dc.identifier.issn1369-703Xspa
dc.identifier.urihttp://hdl.handle.net/10784/7257
dc.descriptionThe impact of flask geometry on Streptomyces lividans growth and morphology, production and Omannosylation of a recombinant O-glycoprotein (APA from Mycobacterium tuberculosis) was described and associated to the evolution of the volumetric power input (P/V) in three shake flask geometries -- During the exponential growth, the highest P/V was found in baffled flasks (BF) with 0.51 kW/m3, followed by coiled flasks (CF) with 0.44 kW/m3 and normal Erlenmeyer flasks (NF) with 0.20 kW/m3 (flasks volume of 250 mL, filling with 50 mL and agitated at 150 rpm) -- During the stationary phase, P/V decreased 20% in BF and CF, but increased two times in NF, surely due to changes in mycelial morphology and its effects on rheology -- Also, NF cultures were carried out at a filling volume and agitation of 15 mL, 150 rpm (15 mL-NF), and 25 mL, 168 rpm (25 mL-NF), in order to raise P/V closely to the values obtained in CF -- However, different growth, morphology and recombinant protein productivity were obtained -- These data indicate that P/V is not a definitive parameter that can determine bacteria growth and morphology, not even glycoprotein production -- But it can be proposed that the oxygen transfer in the center of the pellets and hydromechanical stress might be the more relevant parameters than P/Vspa
dc.language.isoengeng
dc.publisherElsevierspa
dc.relation.ispartofBiochemical Engineering Journal , Volume 90spa
dc.relation.isversionofhttp://dx.doi.org/10.1016/j.bej.2014.06.010spa
dc.rightsUnleashing the power of academic sharing - Sharing Policy - Sharing and Hosting Policy FAQ - Green open access - Journal Embargo Period List (pdf) - Journal Embargo List for UK Authors, Attaching a User License (pdf) - Funding Body Agreementsspa
dc.subjectGeometría del Matrazspa
dc.subjectMorfología micelialspa
dc.titleThe role of volumetric power input in the growth, morphology, and production of a recombinant glycoprotein by Streptomyces lividans in shake flasksspa
dc.typeinfo:eu-repo/semantics/article
dc.typearticleeng
dc.rights.accessrightsinfo:eu-repo/semantics/restrictedAccessspa
dc.publisher.programGrupo de Investigación en Ciencias Biológicas y Bioprocesosspa
dc.subject.lembGLUCOPROTEÍNASspa
dc.subject.lembREOLOGÍA (BIOLOGÍA)spa
dc.subject.lembBIOREACTORESspa
dc.subject.lembMETABOLITOSspa
dc.subject.lembPROTEÍNAS RECOMBINANTESspa
dc.subject.lembINOCULACIÓNspa
dc.publisher.departmentEscuela de Cienciasspa
dc.type.localArtículo
dc.subject.keywordGlycoproteinsspa
dc.subject.keywordRheology (Biology)spa
dc.subject.keywordBioreactorsspa
dc.subject.keywordMetabolitesspa
dc.subject.keywordRecombinant proteinsspa
dc.rights.localAcceso restringidospa
dc.date.accessioned2015-08-13T14:21:16Z
dc.type.hasVersionpublishedVersionspa
dc.type.hasVersionObra publicadaspa
dc.contributor.departmentUniversidad EAFIT. Escuela de Ciencias. Grupo de Investigación en Ciencias Biológicas y Bioprocesos
dc.contributor.departmentUnidad de Bioprocesos, Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México
dc.contributor.departmentCentro de Ciencias Aplicadas y Desarrollo Tecnológico, Universidad Nacional Autónoma de México
dc.contributor.departmentAVT–Biochemical Engineering, RWTH Aachen University
dc.contributor.departmentDepartamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México
dc.contributor.authorGamboa Suasnavart, Ramsés A
dc.contributor.authorServín González, Luis
dc.contributor.authorSoto, Enrique
dc.contributor.authorKlöckner, Wolf
dc.contributor.authorBüchs, Jochen
dc.contributor.authorTrujillo Roldán, Mauricio A
dc.contributor.authorMarín Palacio, Luz Deisy
dc.contributor.authorValdez Cruz, Norma Adriana
dc.contributor.authorCórdova Aguilar, María Soledad
dc.citation.journalTitleBiochemical Engineering Journalspa
dc.citation.volume90spa
dc.citation.spage224spa
dc.citation.epage233spa
dc.identifier.doi10.1016/j.bej.2014.06.010spa


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