Examinando por Materia "protein synthesis"

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    The role of volumetric power input in the growth, morphology, and production of a recombinant glycoprotein by Streptomyces lividans in shake flasks
    (ELSEVIER SCIENCE BV, 2014-09-15) Marin-Palacio, Luz D.; Gamboa-Suasnavart, Ramses A.; Valdez-Cruz, Norma A.; Servin-Gonzalez, Luis; Soledad Cordova-Aguilar, Ma.; Soto, Enrique; Kloeckner, Wolf; Buechs, Jochen; Trujillo-Roldan, Mauricio A.; Marin-Palacio, Luz D.; Gamboa-Suasnavart, Ramses A.; Valdez-Cruz, Norma A.; Servin-Gonzalez, Luis; Soledad Cordova-Aguilar, Ma.; Soto, Enrique; Kloeckner, Wolf; Buechs, Jochen; Trujillo-Roldan, Mauricio A.; Universidad EAFIT. Departamento de Ingeniería de Procesos; Procesos Ambientales (GIPAB)
    The impact of flask geometry on Streptomyces lividans growth and morphology, production and O-mannosylation of a recombinant O-glycoprotein (APA from Mycobacterium tuberculosis) was described and associated to the evolution of the volumetric power input (P/V) in three shake flask geometries. During the exponential growth, the highest P/V was found in baffled flasks (BF) with 0.51kW/m3, followed by coiled flasks (CF) with 0.44kW/m3 and normal Erlenmeyer flasks (NF) with 0.20kW/m3 (flasks volume of 250mL, filling with 50mL and agitated at 150rpm). During the stationary phase, P/V decreased 20% in BF and CF, but increased two times in NF, surely due to changes in mycelial morphology and its effects on rheology. Also, NF cultures were carried out at a filling volume and agitation of 15mL, 150rpm (15mL-NF), and 25mL, 168rpm (25mL-NF), in order to raise P/V closely to the values obtained in CF. However, different growth, morphology and recombinant protein productivity were obtained. These data indicate that P/V is not a definitive parameter that can determine bacteria growth and morphology, not even glycoprotein production. But it can be proposed that the oxygen transfer in the center of the pellets and hydromechanical stress might be the more relevant parameters than P/V. © 2014 Elsevier B.V.
  • No hay miniatura disponible
    Ítem
    The role of volumetric power input in the growth, morphology, and production of a recombinant glycoprotein by Streptomyces lividans in shake flasks
    (ELSEVIER SCIENCE BV, 2014-09-15) Marin-Palacio, Luz D.; Gamboa-Suasnavart, Ramses A.; Valdez-Cruz, Norma A.; Servin-Gonzalez, Luis; Soledad Cordova-Aguilar, Ma.; Soto, Enrique; Kloeckner, Wolf; Buechs, Jochen; Trujillo-Roldan, Mauricio A.; Universidad EAFIT. Departamento de Ciencias; Ciencias Biológicas y Bioprocesos (CIBIOP)
    The impact of flask geometry on Streptomyces lividans growth and morphology, production and O-mannosylation of a recombinant O-glycoprotein (APA from Mycobacterium tuberculosis) was described and associated to the evolution of the volumetric power input (P/V) in three shake flask geometries. During the exponential growth, the highest P/V was found in baffled flasks (BF) with 0.51kW/m3, followed by coiled flasks (CF) with 0.44kW/m3 and normal Erlenmeyer flasks (NF) with 0.20kW/m3 (flasks volume of 250mL, filling with 50mL and agitated at 150rpm). During the stationary phase, P/V decreased 20% in BF and CF, but increased two times in NF, surely due to changes in mycelial morphology and its effects on rheology. Also, NF cultures were carried out at a filling volume and agitation of 15mL, 150rpm (15mL-NF), and 25mL, 168rpm (25mL-NF), in order to raise P/V closely to the values obtained in CF. However, different growth, morphology and recombinant protein productivity were obtained. These data indicate that P/V is not a definitive parameter that can determine bacteria growth and morphology, not even glycoprotein production. But it can be proposed that the oxygen transfer in the center of the pellets and hydromechanical stress might be the more relevant parameters than P/V. © 2014 Elsevier B.V.