Examinando por Materia "greenhouse"

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    Bacillus subtilis EA-CB0575 genome reveals clues for plant growth promotion and potential for sustainable agriculture
    (Springer, 2020-01-01) Franco-Sierra, N.D.; Posada, L.F.; Santa-María, G.; Romero-Tabarez, M.; Villegas-Escobar, V.; Álvarez, J.C.; Universidad EAFIT. Departamento de Ciencias; Biodiversidad, Evolución y Conservación
    Bacillus subtilis is a remarkably diverse bacterial species that displays many ecological functions. Given its genomic diversity, the strain Bacillus subtilis EA-CB0575, isolated from the rhizosphere of a banana plant, was sequenced and assembled to determine the genomic potential associated with its plant growth promotion potential. The genome was sequenced by Illumina technology and assembled using Velvet 1.2.10, resulting in a whole genome of 4.09 Mb with 4332 genes. Genes involved in the production of indoles, siderophores, lipopeptides, volatile compounds, phytase, bacilibactin, and nitrogenase were predicted by gene annotation or by metabolic pathway prediction by RAST. These potential traits were determined using in vitro biochemical tests, finding that B. subtilis EA-CB0575 produces two families of lipopeptides (surfactin and fengycin), solubilizes phosphate, fixes nitrogen, and produces indole and siderophores compounds. Finally, strain EA-CB0575 increased 34.60% the total dry weight (TDW) of tomato plants with respect to non-inoculated plants at greenhouse level. These results suggest that the identification of strain-specific genes and predicted metabolic pathways might explain the strain potential to promote plant growth by several mechanisms of action, accelerating the development of plant biostimulants for sustainable agricultural. © 2020, Springer-Verlag GmbH Germany, part of Springer Nature.
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    Bacillus subtilis EA-CB0575 genome reveals clues for plant growth promotion and potential for sustainable agriculture
    (Springer, 2020-01-01) Franco-Sierra, N.D.; Posada, L.F.; Santa-María, G.; Romero-Tabarez, M.; Villegas-Escobar, V.; Álvarez, J.C.; Universidad EAFIT. Departamento de Ciencias; Ciencias Biológicas y Bioprocesos (CIBIOP)
    Bacillus subtilis is a remarkably diverse bacterial species that displays many ecological functions. Given its genomic diversity, the strain Bacillus subtilis EA-CB0575, isolated from the rhizosphere of a banana plant, was sequenced and assembled to determine the genomic potential associated with its plant growth promotion potential. The genome was sequenced by Illumina technology and assembled using Velvet 1.2.10, resulting in a whole genome of 4.09 Mb with 4332 genes. Genes involved in the production of indoles, siderophores, lipopeptides, volatile compounds, phytase, bacilibactin, and nitrogenase were predicted by gene annotation or by metabolic pathway prediction by RAST. These potential traits were determined using in vitro biochemical tests, finding that B. subtilis EA-CB0575 produces two families of lipopeptides (surfactin and fengycin), solubilizes phosphate, fixes nitrogen, and produces indole and siderophores compounds. Finally, strain EA-CB0575 increased 34.60% the total dry weight (TDW) of tomato plants with respect to non-inoculated plants at greenhouse level. These results suggest that the identification of strain-specific genes and predicted metabolic pathways might explain the strain potential to promote plant growth by several mechanisms of action, accelerating the development of plant biostimulants for sustainable agricultural. © 2020, Springer-Verlag GmbH Germany, part of Springer Nature.
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    Implementation of system operation modes for health management and failure prognosis in cyber-physical systems
    (MDPI AG, 2020-01-01) Ruiz-Arenas, S.; Rusák, Z.; Mejía-Gutierrez, R.; Horváth, I.; Universidad EAFIT. Departamento de Ingeniería de Diseño; Ingeniería de Diseño (GRID)
    Cyber-physical systems (CPSs) have sophisticated control mechanisms that help achieve optimal system operations and services. These mechanisms, imply considering multiple signal inputs in parallel, to timely respond to varying working conditions. Despite the advantages that control mechanisms convey, they bring new challenges in terms of failure prevention. The compensatory action the control exerts cause a fault masking effect, hampering fault diagnosis. Likewise, the multiple information inputs CPSs have to process can affect the timely system response to faults. This article proposes a failure prognosis method, which combines time series-based forecasting methods with statistically based classification techniques in order to investigate system degradation and failure forming on system levels. This method utilizes a new approach based on the concept of the system operation mode (SOM) that offers a novel perspective for health management that allows monitoring the system behavior, through the frequency and duration of SOMs. Validation of this method was conducted by systematically injecting faults in a cyber-physical greenhouse testbed. The obtained results demonstrate that the degradation and fault forming process can be monitored by analyzing the changes of the frequency and duration of SOMs. These indicators made possible to estimate the time to failure caused by various failures in the conducted experiments. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
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    Scale-up from shake flasks to pilot-scale production of the plant growth-promoting bacterium Azospirillum brasilense for preparing a liquid inoculant formulation
    (SPRINGER, 2013-11-01) Trujillo-Roldan, Mauricio A.; Valdez-Cruz, Norma A.; Gonzalez-Monterrubio, Cesar F.; Acevedo-Sanchez, Eduardo V.; Martinez-Salinas, Carlos; Garcia-Cabrera, Ramses I.; Gamboa-Suasnavart, Ramses A.; Marin-Palacio, Luz D.; Villegas, Jesus; Blancas-Cabrera, Abel; Universidad EAFIT. Departamento de Ciencias; Ciencias Biológicas y Bioprocesos (CIBIOP)
    Azospirillum brasilense has industrial significance as a growth promoter in plants of commercial interest. However, there is no report in the literature disclosing a liquid product produced in pilot-scale bioreactors and is able to be stored at room temperature for more than 2 years. The aim of this work was to scale up a process from a shake flask to a 10-L lab-scale and 1,000-L pilot-scale bioreactor for the production of plant growth-promoting bacterium A. brasilense for a liquid inoculant formulation. Furthermore, this work aimed to determine the shelf life of the liquid formulation stored at room temperature and to increase maize crops yield in greenhouses. Under a constant oxygen mass transfer coefficient (K L a), a fermentation process was successfully scaled up from shake flasks to 10- and 1,000-L bioreactors. A concentration ranging from 3.5 to 7.5 × 108 CFU/mL was obtained in shake flasks and bioreactors, and after 2 years stored at room temperature, the liquid formulation showed one order of magnitude decrease. Applications of the cultured bacteria in maize yields resulted in increases of up to 95 % in corncobs and 70 % in aboveground biomass. © 2013 Springer-Verlag Berlin Heidelberg.
  • No hay miniatura disponible
    Ítem
    Scale-up from shake flasks to pilot-scale production of the plant growth-promoting bacterium Azospirillum brasilense for preparing a liquid inoculant formulation
    (SPRINGER, 2013-11-01) Trujillo-Roldan, Mauricio A.; Valdez-Cruz, Norma A.; Gonzalez-Monterrubio, Cesar F.; Acevedo-Sanchez, Eduardo V.; Martinez-Salinas, Carlos; Garcia-Cabrera, Ramses I.; Gamboa-Suasnavart, Ramses A.; Marin-Palacio, Luz D.; Villegas, Jesus; Blancas-Cabrera, Abel; Trujillo-Roldan, Mauricio A.; Valdez-Cruz, Norma A.; Gonzalez-Monterrubio, Cesar F.; Acevedo-Sanchez, Eduardo V.; Martinez-Salinas, Carlos; Garcia-Cabrera, Ramses I.; Gamboa-Suasnavart, Ramses A.; Marin-Palacio, Luz D.; Villegas, Jesus; Blancas-Cabrera, Abel; Universidad EAFIT. Departamento de Ingeniería de Procesos; Procesos Ambientales (GIPAB)
    Azospirillum brasilense has industrial significance as a growth promoter in plants of commercial interest. However, there is no report in the literature disclosing a liquid product produced in pilot-scale bioreactors and is able to be stored at room temperature for more than 2 years. The aim of this work was to scale up a process from a shake flask to a 10-L lab-scale and 1,000-L pilot-scale bioreactor for the production of plant growth-promoting bacterium A. brasilense for a liquid inoculant formulation. Furthermore, this work aimed to determine the shelf life of the liquid formulation stored at room temperature and to increase maize crops yield in greenhouses. Under a constant oxygen mass transfer coefficient (K L a), a fermentation process was successfully scaled up from shake flasks to 10- and 1,000-L bioreactors. A concentration ranging from 3.5 to 7.5 × 108 CFU/mL was obtained in shake flasks and bioreactors, and after 2 years stored at room temperature, the liquid formulation showed one order of magnitude decrease. Applications of the cultured bacteria in maize yields resulted in increases of up to 95 % in corncobs and 70 % in aboveground biomass. © 2013 Springer-Verlag Berlin Heidelberg.