Examinando por Materia "fluorescence imaging"

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    The limiting layer of fish scales: Structure and properties
    (ELSEVIER SCI LTD, 2018-02-01) Arola D.; Murcia S.; Stossel M.; Pahuja R.; Linley T.; Devaraj A.; Ramulu M.; Ossa E.A.; Wang J.; Arola D.; Murcia S.; Stossel M.; Pahuja R.; Linley T.; Devaraj A.; Ramulu M.; Ossa E.A.; Wang J.; Universidad EAFIT. Departamento de Ingeniería de Producción; Materiales de Ingeniería
    Fish scales serve as a flexible natural armor that have received increasing attention across the materials community. Most efforts in this area have focused on the composite structure of the predominately organic elasmodine, and limited work addresses the highly mineralized external portion known as the Limiting Layer (LL). This coating serves as the first barrier to external threats and plays an important role in resisting puncture. In this investigation the structure, composition and mechanical behavior of the LL were explored for three different fish, including the arapaima (Arapaima gigas), the tarpon (Megalops atlanticus) and the carp (Cyprinus carpio). The scales of these three fish have received the most attention within the materials community. Features of the LL were evaluated with respect to anatomical position to distinguish site-specific functional differences. Results show that there are significant differences in the surface morphology of the LL from posterior and anterior regions in the scales, and between the three fish species. The calcium to phosphorus ratio and the mineral to collagen ratios of the LL are not equivalent among the three fish. Results from nanoindentation showed that the LL of tarpon scales is the hardest, followed by the carp and the arapaima and the differences in hardness are related to the apatite structure, possibly induced by the growth rate and environment of each fish.STATEMENT OF SIGNIFICANCE: The natural armor of fish, turtles and other animals, has become a topic of substantial scientific interest. The majority of investigations have focused on the more highly organic layer known as the elasmodine. The present study addresses the highly mineralized external portion known as the Limiting Layer (LL). Specifically, the structure, composition and mechanical behavior of the LL were explored for three different fish, including the arapaima (Arapaima gigas), the tarpon (Megalops atlanticus) and the carp (Cyprinus carpio). Results show that there are significant differences in the surface morphology of the LL from posterior and anterior regions in the scales, and between the three species. In addition, the composition of the LL is also unique among the three fish. Results from nanoindentation showed that the LL of tarpon scales is the hardest, followed by the carp and the arapaima and the differences in hardness are related to the apatite structure, possibly induced by the growth rate and environment of each fish. In addition, a new feature was indentified in the LL, which has not been discussed before. As such, we feel this work is unique and makes a significant contribution to the field. Copyright © 2017 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
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    Vortex-enhanced coherent-illumination phase diversity for phase retrieval in coherent imaging systems
    (OSA - The Optical Society, 2016-04-15) Echeverri-Chacón, S.; Restrepo, R.; Cuartas-Vélez, C.; Uribe-Patarroyo, N.; Universidad EAFIT. Departamento de Ciencias Básicas; Óptica Aplicada
    We propose a phase-retrieval method based on the numerical optimization of a new objective function using coherent phase-diversity images as inputs for the characterization of aberrations in coherent imaging systems. By employing a spatial light modulator to generate multiple-order spiral phase masks as diversities, we obtain an increase in the accuracy of the retrieved phase compared with similar state-of-the-art phase-retrieval techniques that use the same number of input images. We present simulations that show a consistent advantage of our technique, and experimental validation where our implementation is used to characterize a highly aberrated 4F optical system. (C) 2016 Optical Society of America