Examinando por Materia "bacterial protein"

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    Evolutionary and sequence-based relationships in bacterial AdoMet-dependent non-coding RNA methyltransferases
    (BioMed Central Ltd., 2014-01-01) Mosquera-Rendón, J.; Cárdenas-Brito, S.; Pineda, J.D.; Corredor, M.; Benítez-Páez, A.; Mosquera-Rendón, J.; Cárdenas-Brito, S.; Pineda, J.D.; Corredor, M.; Benítez-Páez, A.; Universidad EAFIT. Departamento de Ingeniería de Sistemas; I+D+I en Tecnologías de la Información y las Comunicaciones
    Background: RNA post-transcriptional modification is an exciting field of research that has evidenced this editing process as a sophisticated epigenetic mechanism to fine tune the ribosome function and to control gene expression. Although tRNA modifications seem to be more relevant for the ribosome function and cell physiology as a whole, some rRNA modifications have also been seen to play pivotal roles, essentially those located in central ribosome regions. RNA methylation at nucleobases and ribose moieties of nucleotides appear to frequently modulate its chemistry and structure. RNA methyltransferases comprise a superfamily of highly specialized enzymes that accomplish a wide variety of modifications. These enzymes exhibit a poor degree of sequence similarity in spite of using a common reaction cofactor and modifying the same substrate type. Results: Relationships and lineages of RNA methyltransferases have been extensively discussed, but no consensus has been reached. To shed light on this topic, we performed amino acid and codon-based sequence analyses to determine phylogenetic relationships and molecular evolution. We found that most Class I RNA MTases are evolutionarily related to protein and cofactor/vitamin biosynthesis methyltransferases. Additionally, we found that at least nine lineages explain the diversity of RNA MTases. We evidenced that RNA methyltransferases have high content of polar and positively charged amino acid, which coincides with the electrochemistry of their substrates. Conclusions: After studying almost 12,000 bacterial genomes and 2,000 patho-pangenomes, we revealed that molecular evolution of Class I methyltransferases matches the different rates of synonymous and non-synonymous substitutions along the coding region. Consequently, evolution on Class I methyltransferases selects against amino acid changes affecting the structure conformation. © 2014 Mosquera-Rendón et al.; licensee BioMed Central Ltd.
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    The role of volumetric power input in the growth, morphology, and production of a recombinant glycoprotein by Streptomyces lividans in shake flasks
    (ELSEVIER SCIENCE BV, 2014-09-15) Marin-Palacio, Luz D.; Gamboa-Suasnavart, Ramses A.; Valdez-Cruz, Norma A.; Servin-Gonzalez, Luis; Soledad Cordova-Aguilar, Ma.; Soto, Enrique; Kloeckner, Wolf; Buechs, Jochen; Trujillo-Roldan, Mauricio A.; Universidad EAFIT. Departamento de Ciencias; Ciencias Biológicas y Bioprocesos (CIBIOP)
    The impact of flask geometry on Streptomyces lividans growth and morphology, production and O-mannosylation of a recombinant O-glycoprotein (APA from Mycobacterium tuberculosis) was described and associated to the evolution of the volumetric power input (P/V) in three shake flask geometries. During the exponential growth, the highest P/V was found in baffled flasks (BF) with 0.51kW/m3, followed by coiled flasks (CF) with 0.44kW/m3 and normal Erlenmeyer flasks (NF) with 0.20kW/m3 (flasks volume of 250mL, filling with 50mL and agitated at 150rpm). During the stationary phase, P/V decreased 20% in BF and CF, but increased two times in NF, surely due to changes in mycelial morphology and its effects on rheology. Also, NF cultures were carried out at a filling volume and agitation of 15mL, 150rpm (15mL-NF), and 25mL, 168rpm (25mL-NF), in order to raise P/V closely to the values obtained in CF. However, different growth, morphology and recombinant protein productivity were obtained. These data indicate that P/V is not a definitive parameter that can determine bacteria growth and morphology, not even glycoprotein production. But it can be proposed that the oxygen transfer in the center of the pellets and hydromechanical stress might be the more relevant parameters than P/V. © 2014 Elsevier B.V.
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    Ítem
    The role of volumetric power input in the growth, morphology, and production of a recombinant glycoprotein by Streptomyces lividans in shake flasks
    (ELSEVIER SCIENCE BV, 2014-09-15) Marin-Palacio, Luz D.; Gamboa-Suasnavart, Ramses A.; Valdez-Cruz, Norma A.; Servin-Gonzalez, Luis; Soledad Cordova-Aguilar, Ma.; Soto, Enrique; Kloeckner, Wolf; Buechs, Jochen; Trujillo-Roldan, Mauricio A.; Marin-Palacio, Luz D.; Gamboa-Suasnavart, Ramses A.; Valdez-Cruz, Norma A.; Servin-Gonzalez, Luis; Soledad Cordova-Aguilar, Ma.; Soto, Enrique; Kloeckner, Wolf; Buechs, Jochen; Trujillo-Roldan, Mauricio A.; Universidad EAFIT. Departamento de Ingeniería de Procesos; Procesos Ambientales (GIPAB)
    The impact of flask geometry on Streptomyces lividans growth and morphology, production and O-mannosylation of a recombinant O-glycoprotein (APA from Mycobacterium tuberculosis) was described and associated to the evolution of the volumetric power input (P/V) in three shake flask geometries. During the exponential growth, the highest P/V was found in baffled flasks (BF) with 0.51kW/m3, followed by coiled flasks (CF) with 0.44kW/m3 and normal Erlenmeyer flasks (NF) with 0.20kW/m3 (flasks volume of 250mL, filling with 50mL and agitated at 150rpm). During the stationary phase, P/V decreased 20% in BF and CF, but increased two times in NF, surely due to changes in mycelial morphology and its effects on rheology. Also, NF cultures were carried out at a filling volume and agitation of 15mL, 150rpm (15mL-NF), and 25mL, 168rpm (25mL-NF), in order to raise P/V closely to the values obtained in CF. However, different growth, morphology and recombinant protein productivity were obtained. These data indicate that P/V is not a definitive parameter that can determine bacteria growth and morphology, not even glycoprotein production. But it can be proposed that the oxygen transfer in the center of the pellets and hydromechanical stress might be the more relevant parameters than P/V. © 2014 Elsevier B.V.