Examinando por Materia "Microscopy"

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    Cristalinidad de mezclas binarias de Polibutileno Tereftalato (PBT) y Polieterimida (PEI) analizadas mediante microscopia de luz polarizada
    (Universidad EAFIT, 2016) Jiménez Zapata, Andrés Steven; Álvarez Láinez, Mónica Lucía
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    Development of a linear diattenuation sensitive semi-autonomous and portable digital lensless holographic microscope
    (Universidad EAFIT, 2022) Lopera Acosta, Maria Josef; Trujillo Anaya, Carlos Alejandro
    This document presents the development of a linear diattenuation sensitive, semi-autonomous and portable digital lensless holographic microscope (DLHM). With the development of this project, we aim to improve the performance of this microscopy technique and to broaden the possibilities for a portable DLHM device. We believe our microscope can potentially be used in the diagnosis of diseases. In order to address this aim, the following aspects were investigated within the scope of this project. i) The design of specialized illumination sources for DLHM that are efficient, mechanically stable, and easy to align. ii) The implementation of a DLHM sensitive to the linear diattenuation response of samples. iii) The study of machine-learning based autofocusing methods to be applied to DLHM holograms. iv) The design of specialized software to acquire and reconstruct DLHM holograms and iiv) the design and manufacture of proper mechanical couplings that integrate the DLHM setup with the cellphone’s digital sensor. Five papers and five proceedings are the resultant products of this thesis project. They constitute part of the core of this thesis and are attached by the end of the document.
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    Enhanced molecular visualization of root colonization and growth promotion by Bacillus subtilis EA-CB0575 in different growth systems
    (Elsevier GmbH, 2018-01-01) Posada, L.F.; Álvarez, J.C.; Romero-Tabarez, M.; de-Bashan, L.; Villegas-Escobar, V.; Universidad EAFIT. Departamento de Ciencias; Ciencias Biológicas y Bioprocesos (CIBIOP)
    Bacillus subtilis EA-CB0575 is a plant growth-promoting bacterium (PGPB) associated with banana and tomato crops. Root colonization is an important trait for PGPB microorganisms and potentiates the bacterial effect related to the mechanisms of plant growth promotion. Therefore, detection of bacterial colonization of roots in different culture systems is important in the study of plant–microorganism interactions. In this study, fluorescent in situ hybridization (FISH) and catalyzed reporter deposition–FISH (CARD–FISH) were evaluated to determine the colonization ability of B. subtilis EA-CB0575 on banana and tomato roots planted on solid and liquid Murashige and Skoog medium (MS(S) and MS(L), respectively) and in soil for tomato plants. Results showed B. subtilis colonization 0–30 days post inoculation for banana and tomato plants in different culture systems with differential distribution of bacterial cells along tomato and banana roots. FISH and CARD–FISH methodologies were both successful in detecting B. subtilis colonies, but CARD–FISH proved to be superior due to its enhanced fluorescence signal. The presence of bacteria correlated with the promotion of plant growth in both plant species, providing clues to relate rhizospheric colonization with improvement in plant growth. FISH and CARD–FISH analysis results suggested the presence of native microbiota on the roots of in vitro banana plants, but not on those of tomato plants. © 2018 Elsevier GmbH