Examinando por Materia "Metabolites"
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Ítem Caracterización de dos metabolitos secundarios aislados de las hojas de Pouteria Caimito(Universidad EAFIT, 2012) Aristizábal Cardona, Juan David; Palacios Mosquera, Carlos Mario; Palacio González, Guillermo LeónÍtem Evaluación del crecimiento de Spirulina platensis UTEX 1926 cultivada en medios salinos, utilizando CO2 como fuente de carbono(Universidad EAFIT, 2017) Rojas Osorio, Daniela; Vargas Betancur, Gabriel Jaime; Sáez Vega, Alex ArmandoThe cell growth of Spirulina platensis and the production their metabolites, are affected by factors such as the supply of nutrients and physical-chemical parameters such as salinity, pH, temperature and light availability -- The reduction of the use of fresh water and the reduction of production costs of Spirulina platensis culture; the use of sea water is implemented, due to its content of several inorganic constituents that are available to the primaries producers such as microalgae and cyanobacteria -- In this study the cell growth of the Spirulina platensis UTEX 1926 and the production of metabolites under various concentrations of salinity, sodium nitrate and dipotassium phosphate were evaluated in order to obtain the most suitable condition and the understanding of physiological that intervene in the metabolism of cyanobacteria -- A Central Composite Design (CCD) was applied and each treatment was performed thrice -- The strain was cultivated in 1000mL flasks, with an air stream enriched with 7% CO2, photoperiod 12:12 under artificial light (LED) with an intensity of 55±4μmol/m2s and temperature on average 27°C±3 -- The results showed that Spirulina platensis UTEX 1926 reached a cellular concentration of 2197mg/L when concentrations of 38,546g/L, 0,727g/L, 0,855g/L salinity, sodium nitrate and dipotassium phosphate respectively were used -- In addition, under these concentrations of nutrients, 203mg/L of proteins, 4mg/L of phycocyanin and 3mg/L of exopolysaccharides were obtained -- Finally the cost per unit of mass is 0,19US for the chosen condition, being a means of low cost compared to the Zarrouk medium (0,74US per unit mass)Ítem Inducible Antibacterial Activity in the Bacillales by Triphenyl Tetrazolium Chloride(Nature Publishing Group, 2020-01-01) Sierra-Zapata L.; Álvarez J.C.; Romero-Tabarez M.; Silby M.W.; Traxler M.F.; Behie S.W.; Pessotti R.C.; Villegas-Escobar V.; Universidad EAFIT. Departamento de Ciencias; Biodiversidad, Evolución y ConservaciónThe world is in the midst of an antimicrobial resistance crisis, driving a need to discover novel antibiotic substances. Using chemical cues as inducers to unveil a microorganism’s full metabolic potential is considered a successful strategy. To this end, we investigated an inducible antagonistic behavior in multiple isolates of the order Bacillales, where large inhibition zones were produced against Ralstonia solanacearum only when grown in the presence of the indicator triphenyl tetrazolium chloride (TTC). This bioactivity was produced in a TTC-dose dependent manner. Escherichia coli and Staphylococcus sp. isolates were also inhibited by Bacillus sp. strains in TTC presence, to a lesser extent. Knockout mutants and transcriptomic analysis of B. subtilis NCIB 3610 cells revealed that genes from the L-histidine biosynthetic pathway, the purine, pyrimidine de novo synthesis and salvage and interconversion routes, were significantly upregulated. Chemical space studied through metabolomic analysis, showed increased presence of nitrogenous compounds in extracts from induced bacteria. The metabolites orotic acid and L-phenylalaninamide were tested against R. solanacearum, E. coli, Staphylococcus sp. and B. subtilis, and exhibited activity against pathogens only in the presence of TTC, suggesting a biotransformation of nitrogenous compounds in Bacillus sp. cells as the plausible cause of the inducible antagonistic behavior. © 2020, The Author(s).Ítem The role of volumetric power input in the growth, morphology, and production of a recombinant glycoprotein by Streptomyces lividans in shake flasks(Elsevier, 2014) Gamboa Suasnavart, Ramsés A; Servín González, Luis; Soto, Enrique; Klöckner, Wolf; Büchs, Jochen; Trujillo Roldán, Mauricio A; Marín Palacio, Luz Deisy; Valdez Cruz, Norma Adriana; Córdova Aguilar, María Soledad; Unidad de Bioprocesos, Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México; Centro de Ciencias Aplicadas y Desarrollo Tecnológico, Universidad Nacional Autónoma de México; AVT–Biochemical Engineering, RWTH Aachen University; Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México; Universidad EAFIT. Departamento de Ciencias; Mauricio A TrujilloRoldán (maurotru@gmail.com); Ciencias Biológicas y Bioprocesos (CIBIOP)The impact of flask geometry on Streptomyces lividans growth and morphology, production and Omannosylation of a recombinant O-glycoprotein (APA from Mycobacterium tuberculosis) was described and associated to the evolution of the volumetric power input (P/V) in three shake flask geometries -- During the exponential growth, the highest P/V was found in baffled flasks (BF) with 0.51 kW/m3, followed by coiled flasks (CF) with 0.44 kW/m3 and normal Erlenmeyer flasks (NF) with 0.20 kW/m3 (flasks volume of 250 mL, filling with 50 mL and agitated at 150 rpm) -- During the stationary phase, P/V decreased 20% in BF and CF, but increased two times in NF, surely due to changes in mycelial morphology and its effects on rheology -- Also, NF cultures were carried out at a filling volume and agitation of 15 mL, 150 rpm (15 mL-NF), and 25 mL, 168 rpm (25 mL-NF), in order to raise P/V closely to the values obtained in CF -- However, different growth, morphology and recombinant protein productivity were obtained -- These data indicate that P/V is not a definitive parameter that can determine bacteria growth and morphology, not even glycoprotein production -- But it can be proposed that the oxygen transfer in the center of the pellets and hydromechanical stress might be the more relevant parameters than P/V