Examinando por Materia "Colony Count"

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    Ítem
    Scale-up from shake flasks to pilot-scale production of the plant growth-promoting bacterium Azospirillum brasilense for preparing a liquid inoculant formulation
    (SPRINGER, 2013-11-01) Trujillo-Roldan, Mauricio A.; Valdez-Cruz, Norma A.; Gonzalez-Monterrubio, Cesar F.; Acevedo-Sanchez, Eduardo V.; Martinez-Salinas, Carlos; Garcia-Cabrera, Ramses I.; Gamboa-Suasnavart, Ramses A.; Marin-Palacio, Luz D.; Villegas, Jesus; Blancas-Cabrera, Abel; Universidad EAFIT. Departamento de Ciencias; Ciencias Biológicas y Bioprocesos (CIBIOP)
    Azospirillum brasilense has industrial significance as a growth promoter in plants of commercial interest. However, there is no report in the literature disclosing a liquid product produced in pilot-scale bioreactors and is able to be stored at room temperature for more than 2 years. The aim of this work was to scale up a process from a shake flask to a 10-L lab-scale and 1,000-L pilot-scale bioreactor for the production of plant growth-promoting bacterium A. brasilense for a liquid inoculant formulation. Furthermore, this work aimed to determine the shelf life of the liquid formulation stored at room temperature and to increase maize crops yield in greenhouses. Under a constant oxygen mass transfer coefficient (K L a), a fermentation process was successfully scaled up from shake flasks to 10- and 1,000-L bioreactors. A concentration ranging from 3.5 to 7.5 × 108 CFU/mL was obtained in shake flasks and bioreactors, and after 2 years stored at room temperature, the liquid formulation showed one order of magnitude decrease. Applications of the cultured bacteria in maize yields resulted in increases of up to 95 % in corncobs and 70 % in aboveground biomass. © 2013 Springer-Verlag Berlin Heidelberg.
  • No hay miniatura disponible
    Ítem
    Scale-up from shake flasks to pilot-scale production of the plant growth-promoting bacterium Azospirillum brasilense for preparing a liquid inoculant formulation
    (SPRINGER, 2013-11-01) Trujillo-Roldan, Mauricio A.; Valdez-Cruz, Norma A.; Gonzalez-Monterrubio, Cesar F.; Acevedo-Sanchez, Eduardo V.; Martinez-Salinas, Carlos; Garcia-Cabrera, Ramses I.; Gamboa-Suasnavart, Ramses A.; Marin-Palacio, Luz D.; Villegas, Jesus; Blancas-Cabrera, Abel; Trujillo-Roldan, Mauricio A.; Valdez-Cruz, Norma A.; Gonzalez-Monterrubio, Cesar F.; Acevedo-Sanchez, Eduardo V.; Martinez-Salinas, Carlos; Garcia-Cabrera, Ramses I.; Gamboa-Suasnavart, Ramses A.; Marin-Palacio, Luz D.; Villegas, Jesus; Blancas-Cabrera, Abel; Universidad EAFIT. Departamento de Ingeniería de Procesos; Procesos Ambientales (GIPAB)
    Azospirillum brasilense has industrial significance as a growth promoter in plants of commercial interest. However, there is no report in the literature disclosing a liquid product produced in pilot-scale bioreactors and is able to be stored at room temperature for more than 2 years. The aim of this work was to scale up a process from a shake flask to a 10-L lab-scale and 1,000-L pilot-scale bioreactor for the production of plant growth-promoting bacterium A. brasilense for a liquid inoculant formulation. Furthermore, this work aimed to determine the shelf life of the liquid formulation stored at room temperature and to increase maize crops yield in greenhouses. Under a constant oxygen mass transfer coefficient (K L a), a fermentation process was successfully scaled up from shake flasks to 10- and 1,000-L bioreactors. A concentration ranging from 3.5 to 7.5 × 108 CFU/mL was obtained in shake flasks and bioreactors, and after 2 years stored at room temperature, the liquid formulation showed one order of magnitude decrease. Applications of the cultured bacteria in maize yields resulted in increases of up to 95 % in corncobs and 70 % in aboveground biomass. © 2013 Springer-Verlag Berlin Heidelberg.