Examinando por Materia "C"

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    Ceratocystis cacaofunesta genome analysis reveals a large expansion of extracellular phosphatidylinositol-specific phospholipase-C genes (PI-PLC)
    (BioMed Central Ltd., 2018-01-17) Molano, E.P.L.; Cabrera, O.G.; Jose, J.; do Nascimento, L.C.; Carazzolle, M.F.; Teixeira, P.J.P.L.; Alvarez, J.C.; Tiburcio, R.A.; Tokimatu Filho, P.M.; de Lima, G.M.A.; Guido, R.V.C.; Corrêa, T.L.R.; Leme, A.F.P.; Mieczkowski, P.; Pereira, G.A.G.; Universidad EAFIT. Departamento de Ciencias; Biodiversidad, Evolución y Conservación
    Background: The Ceratocystis genus harbors a large number of phytopathogenic fungi that cause xylem parenchyma degradation and vascular destruction on a broad range of economically important plants. Ceratocystis cacaofunesta is a necrotrophic fungus responsible for lethal wilt disease in cacao. The aim of this work is to analyze the genome of C. cacaofunesta through a comparative approach with genomes of other Sordariomycetes in order to better understand the molecular basis of pathogenicity in the Ceratocystis genus. Results: We present an analysis of the C. cacaofunesta genome focusing on secreted proteins that might constitute pathogenicity factors. Comparative genome analyses among five Ceratocystidaceae species and 23 other Sordariomycetes fungi showed a strong reduction in gene content of the Ceratocystis genus. However, some gene families displayed a remarkable expansion, in particular, the Phosphatidylinositol specific phospholipases-C (PI-PLC) family. Also, evolutionary rate calculations suggest that the evolution process of this family was guided by positive selection. Interestingly, among the 82 PI-PLCs genes identified in the C. cacaofunesta genome, 70 genes encoding extracellular PI-PLCs are grouped in eight small scaffolds surrounded by transposon fragments and scars that could be involved in the rapid evolution of the PI-PLC family. Experimental secretome using LC-MS/MS validated 24% (86 proteins) of the total predicted secretome (342 proteins), including four PI-PLCs and other important pathogenicity factors. Conclusion: Analysis of the Ceratocystis cacaofunesta genome provides evidence that PI-PLCs may play a role in pathogenicity. Subsequent functional studies will be aimed at evaluating this hypothesis. The observed genetic arsenals, together with the analysis of the PI-PLC family shown in this work, reveal significant differences in the Ceratocystis genome compared to the classical vascular fungi, Verticillium and Fusarium. Altogether, our analyses provide new insights into the evolution and the molecular basis of plant pathogenicity. © 2018 The Author(s).
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    ReWeb3D - Enabling desktop 3D applications to run in the web
    (2013-01-01) Glander, T.; Moreno, A.; Aristizabal, M.; Congote, J.; Posada, J.; Garcia-Alonso, A.; Ruiz, O.; Universidad EAFIT. Departamento de Ingeniería Mecánica; Laboratorio CAD/CAM/CAE
    Currently, 3D rendering is accessible within Web browsers through open standards such as WebGL, X3D, and X3DOM. At the same time, there is wealth of mature desktop software which comprises algorithms, data structures, user interfaces, databases, etc. It is a challenge to reuse such desktop software using the Web visualization resources. In response to this challenge, this article presents a novel framework, called ReWeb3D, which minimizes the redevelopment for migration of existing 3D applications to the Web. The redeployed application runs on a Web server. ReWeb3D captures low-level graphic calls including geometry, texture, and shader programs. The captured content is then served as a WebGL-enabled web page that conveys full interactivity to the client. By splitting the graphics pipeline between client and server, the workload can be balanced, and high-level implementation details and 3D content are hidden. The feasibility of ReWeb3D has been tested with applications which use OpenSceneGraph as rendering platform. The approach shows good results for applications with large data sets (e.g. geodata), but is less suited for applications intensive in animations (e.g. games). Copyright © ACM 978-1-4503-2133-4/13/06 $15.00.