Examinando por Autor "Valdez Cruz, Norma Adriana"

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    Ítem
    The role of volumetric power input in the growth, morphology, and production of a recombinant glycoprotein by Streptomyces lividans in shake flasks
    (Elsevier, 2014) Gamboa Suasnavart, Ramsés A; Servín González, Luis; Soto, Enrique; Klöckner, Wolf; Büchs, Jochen; Trujillo Roldán, Mauricio A; Marín Palacio, Luz Deisy; Valdez Cruz, Norma Adriana; Córdova Aguilar, María Soledad; Unidad de Bioprocesos, Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México; Centro de Ciencias Aplicadas y Desarrollo Tecnológico, Universidad Nacional Autónoma de México; AVT–Biochemical Engineering, RWTH Aachen University; Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México; Universidad EAFIT. Departamento de Ciencias; Mauricio A TrujilloRoldán (maurotru@gmail.com); Ciencias Biológicas y Bioprocesos (CIBIOP)
    The impact of flask geometry on Streptomyces lividans growth and morphology, production and Omannosylation of a recombinant O-glycoprotein (APA from Mycobacterium tuberculosis) was described and associated to the evolution of the volumetric power input (P/V) in three shake flask geometries -- During the exponential growth, the highest P/V was found in baffled flasks (BF) with 0.51 kW/m3, followed by coiled flasks (CF) with 0.44 kW/m3 and normal Erlenmeyer flasks (NF) with 0.20 kW/m3 (flasks volume of 250 mL, filling with 50 mL and agitated at 150 rpm) -- During the stationary phase, P/V decreased 20% in BF and CF, but increased two times in NF, surely due to changes in mycelial morphology and its effects on rheology -- Also, NF cultures were carried out at a filling volume and agitation of 15 mL, 150 rpm (15 mL-NF), and 25 mL, 168 rpm (25 mL-NF), in order to raise P/V closely to the values obtained in CF -- However, different growth, morphology and recombinant protein productivity were obtained -- These data indicate that P/V is not a definitive parameter that can determine bacteria growth and morphology, not even glycoprotein production -- But it can be proposed that the oxygen transfer in the center of the pellets and hydromechanical stress might be the more relevant parameters than P/V
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    Ítem
    Scale-up from shake flasks to pilot-scale production of the plant growth-promoting bacterium Azospirillum brasilense for preparing a liquid inoculant formulation
    (Springer Verlag, 2013) Villegas, Jesús; Trujillo Roldán, Mauricio Alberto; Blancas Cabrera, Abel; Gamboa Suasnavart, Ramsés Adolfo; García Cabrera, Ramsés I.; Acevedo Sánchez, Eduardo V.; Valdez Cruz, Norma Adriana; González Monterrubio, César Fernando; Martínez Salinas, Carlos; Marín Palacio, Luz Deisy; Unidad de Bioprocesos, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México; Biofábrica Siglo XXI S.A. de C.V., Carretera México-Oaxaca Km 106, Col; Universidad EAFIT. Departamento de Ciencias; Mauricio A. Trujillo Roldán (maurotru@biomedicas.unam.mx); Ciencias Biológicas y Bioprocesos (CIBIOP)
    Azospirillum brasilense has industrial significance as a growth promoter in plants of commercial interest -- However, there is no report in the literature disclosing a liquid product produced in pilot-scale bioreactors and is able to be stored at room temperature for more than 2 years -- The aim of this work was to scale up a process from a shake flask to a 10- L lab-scale and 1,000-L pilot-scale bioreactor for the production of plant growth-promoting bacterium A -- brasilense for a liquid inoculant formulation -- Furthermore, this work aimed to determine the shelf life of the liquid formulation stored at room temperature and to increase maize crops yield in greenhouses -- Under a constant oxygen mass transfer coefficient (KLa), a fermentation process was successfully scaled up from shake flasks to 10- and 1,000-L bioreactors -- A concentration ranging from 3.5 to 7.5×108 CFU/mL was obtained in shake flasks and bioreactors, and after 2 years stored at room temperature, the liquid formulation showed one order of magnitude decrease -- Applications of the cultured bacteria in maize yields resulted in increases of up to 95 % in corncobs and 70 % in aboveground biomass