Escuela de Ciencias Aplicadas e Ingeniería

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https://hdl.handle.net/10784/5070

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Examinando Escuela de Ciencias Aplicadas e Ingeniería por Autor "Acevedo Sánchez, Eduardo V."

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    Scale-up from shake flasks to pilot-scale production of the plant growth-promoting bacterium Azospirillum brasilense for preparing a liquid inoculant formulation
    (Springer Verlag, 2013) Villegas, Jesús; Trujillo Roldán, Mauricio Alberto; Blancas Cabrera, Abel; Gamboa Suasnavart, Ramsés Adolfo; García Cabrera, Ramsés I.; Acevedo Sánchez, Eduardo V.; Valdez Cruz, Norma Adriana; González Monterrubio, César Fernando; Martínez Salinas, Carlos; Marín Palacio, Luz Deisy; Unidad de Bioprocesos, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México; Biofábrica Siglo XXI S.A. de C.V., Carretera México-Oaxaca Km 106, Col; Universidad EAFIT. Departamento de Ciencias; Mauricio A. Trujillo Roldán (maurotru@biomedicas.unam.mx); Ciencias Biológicas y Bioprocesos (CIBIOP)
    Azospirillum brasilense has industrial significance as a growth promoter in plants of commercial interest -- However, there is no report in the literature disclosing a liquid product produced in pilot-scale bioreactors and is able to be stored at room temperature for more than 2 years -- The aim of this work was to scale up a process from a shake flask to a 10- L lab-scale and 1,000-L pilot-scale bioreactor for the production of plant growth-promoting bacterium A -- brasilense for a liquid inoculant formulation -- Furthermore, this work aimed to determine the shelf life of the liquid formulation stored at room temperature and to increase maize crops yield in greenhouses -- Under a constant oxygen mass transfer coefficient (KLa), a fermentation process was successfully scaled up from shake flasks to 10- and 1,000-L bioreactors -- A concentration ranging from 3.5 to 7.5×108 CFU/mL was obtained in shake flasks and bioreactors, and after 2 years stored at room temperature, the liquid formulation showed one order of magnitude decrease -- Applications of the cultured bacteria in maize yields resulted in increases of up to 95 % in corncobs and 70 % in aboveground biomass